Identification of Melanoma Stem Cells in Long-term Cultures and of SOX6 as a Specific Biomarker for these Stem Cells

Nascimento, Telma

doi:10.24218/jcet.2015.07

Cited by 2 publications

(3 citation statements)

References 39 publications

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“…Here, we demonstrate that the intermediate state, corresponding to the transitory subset described by Tsoi et al 20 , represents a stable and distinct cell state with underlying activity of SOX6, NFATC2, EGR3, ELF1 and ETV4 GRNs (although the latter two are not specific). These TFs have previously been linked to a more aggressive/dedifferentiated phenotype in cancer and/or in melanoma specifically 32,[57][58][59] , which confirms that these cultures are in-between the melanocytic and the more deleterious mesenchymal-like state. We also determine that Hoek's cohort B cell lines and A375 display enriched activity of SOX11 and TFAP2B, and represent Tsoi's neural crest-like subgroup.…”

Section: Discussionsupporting

confidence: 62%

Robust gene expression programs underlie recurrent cell states and phenotype switching in melanoma

et al. 2020

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Melanoma cells can switch between a melanocytic and mesenchymal-like state. Scattered evidence indicates that additional, intermediate state(s) may exist. To search for such states and decipher their underlying gene regulatory network (GRN), we studied ten melanoma cultures by single-cell RNA-seq, and 26 additional cultures by bulk RNA-seq. Although each culture exhibited a unique transcriptome, we identified shared GRNs that underlie the extreme melanocytic and mesenchymal states, and the intermediate state. This intermediate state is corroborated by a distinct chromatin landscape and governed by the transcription factors SOX6, NFATC2, EGR3, ELF1 and ETV4. Single-cell migration assays confirmed its intermediate migratory phenotype. By time-series sampling of single cells after knockdown of SOX10, we unravelled the sequential and recurrent arrangement of GRNs during phenotype switching. Jointly, these analyses indicate that an intermediate state exists and is driven by a distinct and stable "mixed" GRN rather than being a symbiotic, heterogeneous mix of cells.

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Section: Discussionsupporting

confidence: 62%

Robust gene expression programs underlie recurrent cell states and phenotype switching in melanoma

et al. 2020

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“…In a cancer cell line expression study, SOX6 was found to be highly expressed in melanoma cells but was not detectable in eight other cancers [86]. Additionally, SOX6 was identified as a candidate melanoma driver gene [72] in a screen and SOX6 may be a melanoma stem cell marker [51].…”

Section: Figure Andmentioning

confidence: 99%

“…Law MH 1* , Bishop DT 2* , Lee JE 3# , Brossard M 4,5# , Martin NG 6 , Moses EK 7 , Song F 8 , Barrett JH 2 , Kumar R 9 , Easton DF 10 , Pharoah PD 11 , Swerdlow AJ 12,13 , Kypreou KP 14 , Taylor JC 2 , Harland M 2 , Randerson-Moor J 2 , Akslen LA 15,16 , Andresen PA 17 , Avril MF 18 , Azizi E 19,20 , Scarrà GB 21,22 , Brown KM 23 , Dȩbniak T 24 , Duffy DL 6 , Elder DE 25 , Fang S 3 , Friedman E 20 , Galan P 26 , Ghiorzo P 21,22 , Gillanders EM 27 , Goldstein AM 23 , Gruis NA 28 35,37 , Mann GJ 38 , Molven A 16,39 , Montgomery GW 40 , Novaković S 41 , Olsson H 42,43 , Puig S 44,45 , Puig-Butille JA 44,45 , Wu W 46,47 , , Qureshi AA 48 , Radford-Smith GL 49,50,51 , van der Stoep N 52 , van Doorn R 28 , Whiteman DC 53 , Craig JE 54 , Schadendorf D 55,56 , Simms LA 47 , Burdon KP 57 , Nyholt DR 40,58 , Pooley KA 10 , Orr N 59 , Stratigos AJ 14 , Cust AE 60 , Ward SV 7 , Hayward NK 61 , Han J 46,47 , Schulze HJ…”

Section: List Of Members Of the Melanoma Meta-analysis Consortiummentioning

confidence: 99%

Overlapping genetic architecture between Parkinson disease and melanoma

et al. 2019

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Epidemiologic studies have reported inconsistent results regarding an association between Parkinson disease (PD) and cutaneous melanoma (melanoma). Identifying shared genetic architecture between these diseases can support epidemiologic findings and identify common risk genes and biological pathways. Here we apply polygenic, linkage disequilibrium-informed methods to the largest available case-control, genome-wide association study summary statistic data for melanoma and PD. We identify positive and significant genetic correlation (correlation: 0.17, 95% CI 0.10 to 0.24; P = 4.09 × 10-06) between melanoma and PD. We further demonstrate melanoma and PD-inferred gene expression to overlap across tissues (correlation: 0.14, 95% CI 0.06 to 0.22; P = 7.87 × 10-04), and highlight seven genes including PIEZO1, TRAPPC2L, and SOX6 as potential mediators of the genetic correlation between melanoma and PD. These findings demonstrate specific, shared genetic architecture between PD and melanoma that manifests at the level of gene expression.

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Identification of Melanoma Stem Cells in Long-term Cultures and of SOX6 as a Specific Biomarker for these Stem Cells

Cited by 2 publications

References 39 publications

Robust gene expression programs underlie recurrent cell states and phenotype switching in melanoma

Robust gene expression programs underlie recurrent cell states and phenotype switching in melanoma

Overlapping genetic architecture between Parkinson disease and melanoma

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