Identification of MHC class IIβ resistance/susceptibility alleles to Aeromonas salmonicida in brook charr (Salvelinus fontinalis)

Croisetière, Sébastien; Tarte, Philippe D'Autray; Bernatchez, Louis; Belhumeur, Pierre

doi:10.1016/j.molimm.2008.03.007

Cited by 56 publications

(43 citation statements)

References 60 publications

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“…MHC IIA and IIB were also highly polymorphic as showed by the large number of alleles in spotted halibut. Similar polymorphic features have been reported in various fish species either in the ORF or exon 2 (Hardee et al 1995;Fish Physiol Biochem (2011) 37:767-780 777 Chen 2006;Croisetière et al 2008;Xu et al 2009Xu et al , 2010. Concerning the number of genomic MHC IIA and IIB genes, the present study revealed the presence of up to three different class IIA cDNA sequences in a single individual and five different class IIB cDNA sequences in a single individual of spotted halibut.…”

Section: Discussionsupporting

confidence: 87%

“…Changes to amino acids of the PBR may result in altered binding capability and subsequent pathogen recognition abilities of MHC molecules (Brown et al 1993). Accumulated evidence suggests that polymorphism in MHC genes was strongly associated with resistance/susceptibility to diseases in mammals and non-mammalian species (Bacon 1987;Palti et al 2001;Grimholt et al 2004;Wynne et al 2007;Croisetière et al 2008;Rakus et al 2009;Xu et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Major histocompatibility complex class IIA and IIB genes of the spotted halibut Verasper variegatus: genomic structure, molecular polymorphism, and expression analysis

Jiang

Han

et al. 2011

Fish Physiol Biochem

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The major histocompatibility complex (MHC) is a large genomic region characterized by extremely high polymorphism and its association with resistance/susceptibility to disease in vertebrates. In this study, the full lengths of MHC IIA and IIB cDNA were obtained from spotted halibut (Verasper variegates) by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) approaches. The genomic structure, molecular polymorphism, and expression patterns were examined to study MHC II gene functions in fish. As in other teleosts, the genomic structure of the spotted halibut MHC IIA contained 4 exons and 3 introns. The deduced amino acid sequence of the class IIA molecule shared 28-79% similarity with those of teleosts and mammals. Nine class IIA alleles were identified from five individuals. Three alleles originating from a single individual suggested the existence of at least two class IIA loci in the genome. Six exons and 5 introns were identified from spotted halibut MHC IIB, and the deduced amino acid sequence shared 33-79% similarity with those of teleosts and mammals. Twelve alleles were identified, among which five were observed in a single individual, which suggested at least three class IIB loci. Quantitative real-time PCR analysis revealed the presence of class IIA and IIB transcripts in nine normal tissues with high expression level in kidney and gill. Furthermore, MHC IIA and IIB are probably two candidates of immune molecules involved in the acute-phase response in spotted halibut, because their transcriptional levels were significantly up-regulated in blood and liver after bacterial challenge.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Major histocompatibility complex class IIA and IIB genes of the spotted halibut Verasper variegatus: genomic structure, molecular polymorphism, and expression analysis

Jiang

Han

et al. 2011

Fish Physiol Biochem

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“…MHC is a large genomic region consisting of closely linked and highly polymorphic loci that play a central role in the immune system of vertebrates (Croisetiere et al 2008). There is a high degree of allelic polymorphism and a significant association with disease resistance and susceptibility (Zhang et al 2006b;Wynne et al 2007;Rakus et al 2009;Du et al 2011;Banat et al 2012;Tang et al 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Major histocompatibility complex (MHC) loci encode cellular glycoproteins responsible for presenting self or non-self antigens to T cell receptors, and thereby initiate immune responses in invertebrates (Srisapoome et al 2004;Croisetiere et al 2008). MHC molecules, in general, are divided into two main subgroups according to their chemical structures and molecular functions (Rothbard & Gefter 1991).…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning, genomic structure, polymorphism and expression analysis of major histocompatibility complex class IIA gene of swamp eel Monopterus albus

Sun

Meng

et al. 2013

Biologia

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Major histocompatibility complex (MHC) class II molecules play an important role in the immune response of vertebrates. In this paper, full-length MHC IIA cDNA was isolated from swamp eel (Monopterus albus) by rapid amplification of cDNA ends PCR. The genomic structure, molecular polymorphism, tissue distribution, and immune response of the MHC IIA gene to bacterial challenge were investigated. The full-length cDNA (GenBank accession No.: KC616308) is 1,509 bp in length including an 83 bp-long 5' untranslated region (UTR) and a 709 bp-long 3' UTR, which encoded a 238 amino acids protein. In the 2,339 bp-long MHC IIA genomic DNA, four exons and three introns were identified. Sequence comparison exhibited that the deduced amino acid sequence shared 27.1-66.3% identity with those of other species. Seven alleles were identified from five healthy individuals. Number of alleles per individual diversified from two to five. Five different 5' UTR sequences and two different 3' UTR sequences from one individual may infer the existence of five loci at least. Real-time quantitative PCR demonstrated that swamp eel MHC IIA transcripts were ubiquitously expressed in ten tissues, but the expression level was distinctly different. Significant changes were observed in liver, spleen, kidney and intestine after challenged with pathogenic bacteria Aeromonas hydrophilia.

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“…Previous studies have focused on *215 bp region of this exon that includes part of the peptide binding region. An additional PBR region of high polymorphism at the 5 0 end of exon 2 (Croisetiere et al 2008) makes primer development difficult for the entire exon 2, especially for a single set of primers that amplify a broad spectrum of taxa.…”

mentioning

confidence: 99%

Full length MHC IIβ exon 2 primers for salmonids: a new resource for next generation sequencing

Pavey

Lamaze

Garant

et al. 2011

Conservation Genet Resour

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Major histocompatibility complex (MHC) genes are often implicated in disease resistance, sexual selection and local adaptation in salmonids, a highly studied and socio-economically important taxa. However, genotyping highly polymorphic genes is difficult, expensive, and prone to PCR and cloning artifacts that can result in false alleles. With the advent of next generation sequencing, it is possible to effectively ''clone'' PCR products in a massively parallel fashion with the use of individually-tagged fusion primers. Primers that amplify a gene of interest across a variety of taxa, a single set of 50-150 primers can facilitate cost efficient genotyping and become a common lab resource. Here we developed MHC IIb exon 2 primers for full-length amplification, including an additional PBR region, and demonstrate effectiveness with representatives from five genera of salmonids. These primers may facilitate parallel next generation sequencing for efficient, cost effective, and accurate genotyping of this complex locus.

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Identification of MHC class IIβ resistance/susceptibility alleles to Aeromonas salmonicida in brook charr (Salvelinus fontinalis)

Cited by 56 publications

References 60 publications

Major histocompatibility complex class IIA and IIB genes of the spotted halibut Verasper variegatus: genomic structure, molecular polymorphism, and expression analysis

Major histocompatibility complex class IIA and IIB genes of the spotted halibut Verasper variegatus: genomic structure, molecular polymorphism, and expression analysis

Molecular cloning, genomic structure, polymorphism and expression analysis of major histocompatibility complex class IIA gene of swamp eel Monopterus albus

Full length MHC IIβ exon 2 primers for salmonids: a new resource for next generation sequencing

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