Identification of microorganisms involved in reductive dehalogenation of chlorinated ethenes in an anaerobic microbial community

Yang, Young-Soo; Pesaro, Manuel; Sigler, William V.; Zeyer, Josef

doi:10.1016/j.watres.2005.07.010

Cited by 61 publications

(63 citation statements)

References 51 publications

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“…The detection of some initial mass imbalance with PCE to TCE conversion in some wells is not unusual as this has been observed in other similar studies (Daprato et al 2007;Yang et al 2005). However, in this study, the system was stabilized after 20 days of incubation.…”

Section: Reductive Dechlorination Of Chlorinated Compoundssupporting

confidence: 82%

Site-specific pre-evaluation of bioremediation technologies for chloroethene degradation

Patil

Adetutu²,

Sheppard³

et al. 2013

Int. J. Environ. Sci. Technol.

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Groundwater systems are important sources of water for drinking and irrigation purposes. Unfortunately, human activities have led to widespread groundwater contamination by chlorinated compounds such as tetrachloroethene (PCE). Chloroethenes are extremely harmful to humans and the environment due to their carcinogenic properties. Therefore, this study investigated the potential for bioremediating PCE-contaminated groundwater using laboratory-based biostimulation (BS) and biostimulationbioaugmentation (BS-BA) assays. This was carried out on groundwater samples obtained from a PCE-contaminated site which had been unsuccessfully treated using chemical oxidation. BS resulted in complete dechlorination by week 21 compared to controls which had only 30 % PCE degradation. BS also led to a approximately threefold increase in 16S rRNA gene copies compared to the controls. However, the major bacterial dechlorinating group, Dehalococcoides (Dhc), was undetectable in PCE-contaminated groundwater. This suggested that dechlorination in BS samples was due to indigenous non-Dhc dechlorinators. Application of the BS-BA strategy with Dhc as the augmenting organism resulted in complete dechlorination by week 17 with approximately twofold to threefold increase in 16S rRNA and Dhc gene abundance. Live/dead cell counts (LDCC) showed 70-80 % viability in both treatments indicating active growth of potential dechlorinators. The LDCC was strongly correlated with cell copy numbers (r [ 0.95) suggesting its potential use for low-cost monitoring of bioremediation. This study also shows the dechlorinating potential of indigenous non-Dhc groups can be successfully exploited for PCE decontamination while demonstrating the applicability of microbiological and chemical methodologies for preliminary site assessments prior to field-based studies.

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Section: Reductive Dechlorination Of Chlorinated Compoundssupporting

confidence: 82%

Site-specific pre-evaluation of bioremediation technologies for chloroethene degradation

Patil

Adetutu²,

Sheppard³

et al. 2013

Int. J. Environ. Sci. Technol.

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“…Sporomusa ovata has been shown to dechlorinate PCE to TCE concomitant with homoacetogenesis from methanol and carbon dioxide (46). Sporomusa species phylotypes have also previously been found in chlorinated-ethene-dechlorinating mixed cultures (5,47).…”

Section: Discussionmentioning

confidence: 96%

Monitoring Abundance and Expression of “ Dehalococcoides ” Species Chloroethene-Reductive Dehalogenases in a Tetrachloroethene-Dechlorinating Flow Column

Behrens

Azizian

McMurdie

et al. 2008

Appl Environ Microbiol

127

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We investigated the distribution and activity of chloroethene-degrading microorganisms and associated functional genes during reductive dehalogenation of tetrachloroethene to ethene in a laboratory continuousflow column. Using real-time PCR, we quantified "Dehalococcoides" species 16S rRNA and chloroethenereductive dehalogenase (RDase) genes (pceA, tceA, vcrA, and bvcA) in nucleic acid extracts from different sections of the column. Dehalococcoides 16S rRNA gene copies were highest at the inflow port [(3.6 ؎ 0.6) ؋ 10 6 (mean ؎ standard deviation) per gram soil] where the electron donor and acceptor were introduced into the column. The highest transcript numbers for tceA, vcrA, and bvcA were detected 5 to 10 cm from the column inflow. bvcA was the most highly expressed of all RDase genes and the only vinyl chloride reductase-encoding transcript detectable close to the column outflow. Interestingly, no expression of pceA was detected in the column, despite the presence of the genes in the microbial community throughout the column. By comparing the 16S rRNA gene copy numbers to the sum of all four RDase genes, we found that 50% of the Dehalococcoides population in the first part of the column did not contain either one of the known chloroethene RDase genes. Analysis of 16S rRNA gene clone libraries from both ends of the flow column revealed a microbial community dominated by members of Firmicutes and Actinobacteria. Higher clone sequence diversity was observed near the column outflow. The results presented have implications for our understanding of the ecophysiology of reductively dehalogenating Dehalococcoides spp. and their role in bioremediation of chloroethenes.Tetrachloroethene (PCE) and trichloroethene (TCE) are the most-abundant groundwater contaminants in the United States (32). In situ bioremediation is a promising technology for the removal of these chlorinated solvents from contaminated aquifers (6,23,29). Of particular interest for bioremediation are microorganisms of the genus "Dehalococcoides" (1,7,10,11,13,15,31). In addition to other recalcitrant chloroorganic pollutants, Dehalococcoides spp. reductively dechlorinate PCE, TCE, cis-dichloroethene (cDCE), and vinyl chloride (VC) to ethene. While some microbial species other than Dehalococcoides spp. degrade chlorinated solvents, reductive dechlorination of PCE past cDCE has been linked exclusively to members of the genus Dehalococcoides (11,31,36,45).The reduction of chloroethenes by Dehalococcoides spp. is mediated by reductive dehalogenase (RDase) enzymes. While many RDase genes have been identified, only a few have been characterized for their function. Known RDase genes involved in chloroethene reduction are pceA, encoding PCE reductases from Dehalococcoides ethenogenes strain 195 (DET0318; GenBank accession no. NC_002936) (28) and Dehalococcoides sp. strain CBDB1 (cbdB_A1588; GenBank accession no. NC_007356) (8); tceA, encoding TCE reductases from D. ethenogenes strain 195 (DET0079; GenBank accession no. NC_002936) (27) and Dehalococcoides sp. s...

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“…The dechlorination of chlorinated ethenes is facilitated by certain microorganisms through dehalorespiration under anaerobic conditions (Yang et al 2005). Thus, since this process has the potential of detoxifying PCE and TCE, it has been recognized as a promising approach for the cleanup of contaminated sites and extensively studied for in situ bioremediation.…”

Section: Reductive Dechlorination Of Pcementioning

confidence: 99%

Complete reductive dechlorination of tetrachloroethene to ethene by anaerobic microbial enrichment culture developed from sediment

et al. 2010

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A mixed, anaerobic microbial enrichment culture, AMEC-4P, was developed that uses lactate as the electron donor for the reductive dechlorination of tetrachloroethene (PCE) to ethene. AMEC-4P consistently and completely converted 2 mM PCE to cis-1,2-dichloroethene (cis-DCE) within 13 days, and the intermediate, cis-DCE, was then completely dechlorinated to ethene after 130 days. Dechlorination rates for PCE to cis-DCE, cis-DCE to VC, and VC to ethene were 243, 27, and 41 μmol/l/day, respectively. Geobacter lovleyi and a Dehalococcoides sp. were identified from their 16S rRNA sequences to be the dominant phylotypes in AMEC-4P.

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Identification of microorganisms involved in reductive dehalogenation of chlorinated ethenes in an anaerobic microbial community

Cited by 61 publications

References 51 publications

Site-specific pre-evaluation of bioremediation technologies for chloroethene degradation

Site-specific pre-evaluation of bioremediation technologies for chloroethene degradation

Monitoring Abundance and Expression of “ Dehalococcoides ” Species Chloroethene-Reductive Dehalogenases in a Tetrachloroethene-Dechlorinating Flow Column

Complete reductive dechlorination of tetrachloroethene to ethene by anaerobic microbial enrichment culture developed from sediment

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