Identification of microRNAs associated with the exogenous spermidine-mediated improvement of high-temperature tolerance in cucumber seedlings (Cucumis sativus L.)

Wang, Ying; Guo, Shirong; Wang, Lei; Wang, Liwei; He, Xue‐Ying; Shu, Sheng; Sun, Jian; Lu, Na

doi:10.1186/s12864-018-4678-x

Cited by 18 publications

(25 citation statements)

References 68 publications

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“…Genes respond to high‐temperature stress via changes in expression levels. Wang et al () identified the first set of miRNAs associated with the exogenous Spd‐mediated improvement of high‐temperature tolerance in cucumber seedlings. For example, 34 specific lncRNAs were identified under heat stress and 192 target genes were regulated by lncRNAs, most of which were thermo‐responsive genes (Song et al ).…”

Section: Introductionmentioning

confidence: 99%

Systematic identification and analysis of heat‐stress‐responsive lncRNAs, circRNAs and miRNAs with associated co‐expression and ceRNA networks in cucumber (Cucumis sativus L.)

Guo

Wang

et al. 2019

Physiologia Plantarum

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108

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Researchers have shown that long non‐coding RNAs (lncRNAs) and circular RNAs (circRNAs) act as competitive endogenous RNAs (ceRNAs) and are mutually regulated by competition for binding to common microRNA response elements (MREs). However, a comprehensive identification and analysis of lncRNAs and circRNAs as ceRNAs have not yet been completed in cucumber (Cucumis sativus L.) exposed to high‐temperature stress. In our study, 32 663 coding transcripts, 2085 lncRNAs, 2477 circRNAs and 348 differentially expressed miRNAs were identified using RNA sequencing. In addition, six heat‐stress‐responsive miRNAs (five known and one novel miRNAs) and eight lncRNAs were selected for qPCR to confirm their expression profiles. By analyzing the cis effects of lncRNAs, we constructed a lncRNA‐mRNA co‐expression network. Based on the results, the corresponding lncRNAs play a regulatory role in the stress response in cucumber plants. In our study, the PatMatch software was used to predict the potential function of lncRNAs and circRNAs as ceRNAs. A total of 18 lncRNAs and seven circRNAs were predicted to bind to 114 differentially expressed miRNAs and compete with 359 mRNAs for miRNA binding sites. These mRNAs are predicted to be involved in various pathways, such as plant hormone signal transduction, plant–pathogen interaction and glutathione metabolism. Among them, TCONS_00031790, TCONS_00014332, TCONS_00014717, TCONS_00005674, novel_circ_001543 and novel_circ_000876 may interact with miR9748 by plant hormone signal transduction pathways in response to high‐temperature stress. Moreover, indole‐3‐acetic acid (IAA) and 1‐aminocyclopropane‐l‐carboxylic acid (ACC) levels decreased in the high‐temperature treatment group, indicating that IAA and ethylene signaling might be involved in response to high‐temperature stress. In this study, we conducted a full transcriptomic analysis in response to high‐temperature stress in cucumber and, for the first time, integrated the potential ceRNA functions of lncRNAs/circRNAs. The results provide a basis for studying the potential functions of lncRNAs/circRNAs in response to high‐temperature stress.

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Section: Introductionmentioning

confidence: 99%

Systematic identification and analysis of heat‐stress‐responsive lncRNAs, circRNAs and miRNAs with associated co‐expression and ceRNA networks in cucumber (Cucumis sativus L.)

Guo

Wang

et al. 2019

Physiologia Plantarum

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108

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“…In this study, the effects of exogenous Spd and MGBG on the growth and physiological indicators of resistance in cucumber seedlings under salt stress were analyzed, and the results were in accord with the results of previous studies. Wang et al [32] described the molecular mechanism of exogenous Spd on improving high-temperature tolerance based on the expression of miRNA in cucumber. Sang et al [33] used proteomics studies to analyze the effects of Spd on alleviating high-temperature stress in tomato.…”

Section: Discussionmentioning

confidence: 99%

“…After treatment for 7 d, the growth parameters were measured by the method of Wang et al [32] and Tang et al [52].…”

Section: Salt Stress and Spd Treatmentmentioning

confidence: 99%

Gibberellin mediates spermidine-induced salt tolerance and the expression of GT-3b in cucumber

Wang

Gong

Liu

et al. 2020

Plant Physiology and Biochemistry

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Background Transcription factor GT-3b binds to the GT-1 element in the promoter of Sadenosylmethionine synthase (SAMs) gene, the key gene of spermidine (Spd) biosynthesis, to regulate its expression, thereby increasing the salt tolerance of cucumber plants. Furthermore, exogenous Spd increases the transcript level and protein abundance of SAMs, which promotes the accumulation of endogenous polyamine. However, whether Spd regulation of SAMs depending on GT-3b is largely unknown. Here, we investigated the potential mechanism of Spd in regulating of GT-3b in cucumber seedlings under salt stress. Results Exogenous Spd significantly increased the tolerance to salt stress in cucumber, while its effects were compromised when application of methylglyoxal bis-guanylhydrazone (MGBG), an inhibitor of Spd biosynthesis. GT-3b was significantly induced by Spd under salt stress. The promoter sequence of GT-3b was predicted that there contained cis-acting regulatory elements response to phytohormones, such as gibberellin (GA), salicylic acid (SA) and methyl jasmonate (MeJA). Foliar spray of GA 3 , SA and MeJA could induce the expression of GT-3b. Interestingly, exogenous Spd dramatically induced the expression of genes related to GA biosynthesis, increased the activity of gibberellin oxidase, and promoted the accumulation of GA 3 , but decreased in MGBG-treated plants. Furthermore, application of GA 3 increased the expression of GT-3b and salt tolerance, while blocked when treatment with paclobutrazol (PAZ), the GA biosynthesis inhibitor. In addition, Spd-induced salt tolerance was compromised in PAZ-treated plants. Conclusions Our results suggested that GA mediated Spd-induced salt tolerance and the expression of GT-3b in cucumber. These results provide new perspective for our understanding the molecular mechanism of Spd in regulation of salt tolerance in plants.

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“…Multiple studies of the stress response of vegetables under various abiotic stress conditions have been performed (Gous et al, 2016;Wang et al, 2018;Zhou et al, 2013). Studies of HS on vegetables have mainly focused on HSPs and relative enzymes.…”

mentioning

confidence: 99%

“…Under high-temperature stress, ABA could induce the expression of HSP70, leading to its tolerance to heat in cucumber (Li et al, 2015). MicroRNAs were related to improvements in the high-temperature tolerance of cucumber seedlings (Wang et al, 2018). Ten quantitative trait loci (QTLs) (rate of contribution, 6% to 17%) influencing HS in cucumber have been detected (Chen et al, 2008).…”

mentioning

confidence: 99%

Genetic Analysis and Related Gene Primary Mapping of Heat Stress Tolerance in Cucumber Using Bulked Segregant Analysis

Wang¹,

Liu²,

Jiang³

et al. 2019

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Heat stress (HS) negatively influences plant development and growth, especially production and quality. Cucumber is a widely cultivated plant in the gourd family Cucurbitaceae that is often exposed to high temperatures during summer and protected cultivation. In this study, we performed whole-genome re-sequencing of two pools, one heat-tolerant and one heat-sensitive, of the F2 population derived from L-9 (heat-resistant) and A-16 (heat-sensitive). The genetic analysis showed that the heat resistance of L-9 cucumber seedlings was controlled by a single recessive gene. By combining bulked segregant analysis (BSA) technology, the crucial gene related to HS was preliminarily mapped to a 1.08-Mb region on chromosome 1. To fine-map the locus, Indel markers were designed according to the genomic sequence. Finally, the gene was narrowed to a 550-kb region flanked by two Indel markers, namely Indel-H90 and Indel-H224, that contained 56 candidate genes. Re-sequencing results indicated that 10 candidate genes among the 56 in the candidate region showed single base pair differences in the exons. Quantitative reverse-transcription polymerase chain reaction showed that 6 genes among the 10 candidate genes were significantly decreased when exposed to high temperatures. These results not only were useful for the isolation and characterization of the key genes involved in HS but also provided a basis for understanding the mechanism of heat tolerance regulation.

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Identification of microRNAs associated with the exogenous spermidine-mediated improvement of high-temperature tolerance in cucumber seedlings (Cucumis sativus L.)

Cited by 18 publications

References 68 publications

Systematic identification and analysis of heat‐stress‐responsive lncRNAs, circRNAs and miRNAs with associated co‐expression and ceRNA networks in cucumber (Cucumis sativus L.)

Systematic identification and analysis of heat‐stress‐responsive lncRNAs, circRNAs and miRNAs with associated co‐expression and ceRNA networks in cucumber (Cucumis sativus L.)

Gibberellin mediates spermidine-induced salt tolerance and the expression of GT-3b in cucumber

Genetic Analysis and Related Gene Primary Mapping of Heat Stress Tolerance in Cucumber Using Bulked Segregant Analysis

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