Identification of microRNAs predominately derived from testis and epididymis in human seminal plasma

Hu, Linwei; Wu, Chunlin; Guo, Cuicui; Li, Honggang; Xiong, Chengliang

doi:10.1016/j.clinbiochem.2013.11.009

Cited by 61 publications

(44 citation statements)

References 37 publications

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“…The seminal plasma is a mixture of fluids secreted by the sexual glands (seminal vesicles, the prostate and the bulbourethral glands) and other male sexual organs (testis and epididymis) and provides a nutritive and protective medium for sperm after ejaculation (Juyena & Stelletta 2012). The presence of RNA molecules in human seminal plasma was first identified by Huang and coworkers (Huang et al 2009), and then confirmed by several studies (Huang et al 2009, Weber et al 2010, Wu et al 2012, Xiong 2014. In seminal plasma, these RNA molecules display remarkable stability and resistance to degradation, possibly because of their association with protein complexes or because they are enclosed within membrane-bound nanovesicles referred to as 'exosomes' that are produced by the diverse sexual glands and organs (Sullivan et al 2005, Piehl et al 2013.…”

Section: Discussionmentioning

confidence: 92%

“…Therefore, it is impossible to compare RNA profiles between ejaculated sperm and epididymal sperm in the same species (humans or mice). Seminal plasma is also relatively easy to collect in humans than that in mice, and several RNA species have been identified within seminal plasma (Wu et al 2012, Xiong 2014, Hong et al 2016. However, comparatively little is known about the distribution of RNAs in epididymal sperm, seminal plasma and ejaculated sperm.…”

Section: Introductionmentioning

confidence: 99%

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Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Cai¹,

Wu²,

Shen³

et al. 2017

Reproduction

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The similarities and differences of small RNAs in seminal plasma, epididymal sperm and ejaculated sperm remain largely undefined. We conducted a systematic comparative analysis of small RNA profiles in pig ejaculated sperm, epididymal sperm and seminal plasma and found that the diversity distribution of small RNA species was generally similar, whereas the abundance of small RNAs is dramatically different across the three libraries; miRNAs and small RNAs derived from rRNA, tRNA, small nuclear RNA, 7SK RNA, NRON RNA and cis-regulatory RNA were enriched in the three libraries, but piRNA was absent. A large population of small RNAs from ejaculated sperm are ejaculated sperm specific, and only 8-30% of small RNAs overlapped with those of epididymal sperm or seminal plasma and a small proportion (5-18%) of small RNAs were shared in the three libraries, suggesting that, in addition to the testes, sperm RNAs may also originate from seminal plasma, epididymis as well as other resources. Most miRNAs were co-distributed but differentially expressed across the three libraries, with epididymal sperm exhibiting the highest abundance, followed by ejaculated sperm and seminal plasma. The prediction of target genes of the top 10 highly expressed miRNAs across the three libraries revealed that these miRNAs may be involved in spermatogenesis, zygote development and the interaction between the environment and animals. Our study provides the first description of the similarities and differences of small RNA profiles in ejaculated sperm, epididymal sperm and seminal plasma and indicates that sperm RNA may have origins other than the testes.Reproduction (2017) 153 [785][786][787][788][789][790][791][792][793][794][795][796]

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Cai¹,

Wu²,

Shen³

et al. 2017

Reproduction

View full text Add to dashboard Cite

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“…The gga-miR-34b was found to be the third most highly expressed miRNA, and researches on mice and pigs have also revealed the influence of miR-34b on testicular development1516. Additionally, aca-miR-363 and aca-miR-18a were also found to be high differentially expresser miRNAs in testes of breeder cocks, as well as in other species4445. In addition, compared with the miRNA expression profiles from testes of goat, cow, and mouse222324, we found 13 miRNAs were conserved in testes, which means that these 13 differentially expressed miRNAs could play crucial roles in testicular developments or their functions and the APS could regulate testicular condition by inducing the differential expression of miRNAs.…”

Section: Discussionmentioning

confidence: 95%

Effect of dietary Astragalus Polysaccharide supplements on testicular miRNA expression profiles and enzymatic changes of breeder cocks

Ren

et al. 2017

Sci Rep

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Astragalus Polysaccharide (APS) is an important feed additive due to its immunomodulatory functions. Previous studies have proven that miRNAs play important roles in posttranscriptional gene regulation. Our goals were to identify differentially expressed miRNAs in testes in responses to APS dietary supplements and to find the effects of APS on breeder cock testes. We measured several enzymatic activities in testes and sperm samples and further generated miRNA expression profiles of testes from breeder cocks fed with control diets and extra APS. As a result, we found APS could increase testicular functional activities of marker enzymes. Meanwhile, there were 16 up-regulated and 17 down-regulated miRNAs in APS group, compared with the control group meeting the criteria of P-values < 0.05. Meanwhile, twelve differentially expressed miRNAs were validated by Mir-XTM miRNA RT-qPCR. Further GO and KEGG analyses of target genes for differentially expressed miRNAs revealed that some miRNAs may be involved in testicular nutrient metabolisms and NK cell mediated cytotoxicity pathway. Moreover, the effect of dietary APS supplements on NK cell mediated cytotoxicity pathway was also validated by RT-qPCR. Our results provided a novel insight into the effect of dietary APS supplements on testicular miRNA expression profiles and enzymatic changes of breeder cocks.

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“… Chang et al reported that miR‐22‐5p was differentially expressed between cauda epididymal and ejaculated boar sperm . miR‐342 exhibited higher levels in the seminal plasma and epididymis of normozoospermic men than that in vasectomized men and was 3‐fold upregulated in seminoma than in the normal testicular tissue group . miR‐451a is one of the most abundant miRNA in human mural and cumulus granulosa cells and was reported to be involved in the hypoxia‐induced functional changes of marine medaka testis .…”

Section: Discussionmentioning

confidence: 99%

Differential expression of microRNAs in TM3 Leydig cells of mice treated with brain‐derived neurotrophic factor

Gao

et al. 2017

Cell Biochemistry & Function

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Brain-derived neurotrophic factor (BDNF) is a neurotrophin that can promote the development and proliferation of neurons. BDNF has been found to be involved in male reproduction. Leydig cells in testicular interstitial tissues can secrete testosterone in a luteinizing hormone-dependent manner. We showed that BDNF and its receptor TrkB were expressed in mice TM3 Leydig cells in the present study. Furthermore, BDNF can promote proliferation of mouse TM3 Leydig cells in vitro. Results of microRNA (miRNA) deep sequencing showed that BDNF can alter the expression profile of miRNAs in TM3 Leydig cells. Eighty-three miRNAs were significantly different in the BDNF-treated and control groups (fold change of >2.0 or <0.5, P < 0.05) wherein 40 were upregulated and 43 were downregulated. The expression levels of miR-125a-5p, miR-22-5p, miR-342-59, miR-451a, miR-148a-5p, miR-29b-3p, miR-199b-5p, and miR-145a-5p were further confirmed by quantitative real-time polymerase chain reaction. Bioinformatic analysis revealed that miRNAs regulated a large number of genes with different functions. Pathway analysis indicated that miRNAs participate in the pathways involved in signal transduction, cancer, metabolism, endocrine system, immune system, and nerve system. This study indicated that miRNAs might be involved in the BDNF-regulated cellular functions of Leydig cells.

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Identification of microRNAs predominately derived from testis and epididymis in human seminal plasma

Cited by 61 publications

References 37 publications

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm

Effect of dietary Astragalus Polysaccharide supplements on testicular miRNA expression profiles and enzymatic changes of breeder cocks

Differential expression of microRNAs in TM3 Leydig cells of mice treated with brain‐derived neurotrophic factor

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