Identification of microsatellite markers in a neotropical seasonally dry forest tree, <i>Astronium urundeuva</i> (Anacardiaceae)

Caetano, S.; Balthazar, Patricia; Spichiger, R.; Naciri, Yamama

doi:10.1111/j.1471-8286.2004.00814.x

Cited by 12 publications

(9 citation statements)

References 8 publications

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“…Geoffroea spinosa has vertebrate dispersed fruits, and is found distributed in nearly all the areas described above. As part of a program aiming at analysing the postglacial distribution of NSDF typical species, microsatellites have been developed in this species, together with another characteristic NSDF species, Astronium urundeuva (Caetano et al . 2005).…”

Section: Characteristics Of 11 Optimized Microsatellites In Geoffroeamentioning

confidence: 99%

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Development and characterization of 11 microsatellite markers in a widespread Neotropical seasonally dry forest tree species, Geoffroea spinosa Jacq. (Leguminosae)

Naciri

Caetano

Prado

et al. 2005

Molecular Ecology Notes

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Eleven dinucleotide microsatellites were developed in Geoffroea spinosa (Leguminosae), a widespread tree of the seasonally dry Neotropical forests, and characterized on six populations from Peru, Argentina and Paraguay. Four of them amplified on the Peruvian populations only, probably because of mutations in the microsatellite flanking regions in the other populations. Ten microsatellites were found polymorphic, with within population gene diversities ranging from 0.17 to 0.95, and a number of alleles varying from seven to 19. A significant overall genetic differentiation was also found (θ = 0.212; P < 0.01).

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Section: Characteristics Of 11 Optimized Microsatellites In Geoffroeamentioning

confidence: 99%

“…These 10 polymorphic loci should be useful for the study of G. spinosa genetic structure, and should give us insights into the past history of NSDF together with the study of other species (e.g. Astronium urundeuva , Caetano et al . (2005)).…”

Section: Characteristics Of 11 Optimized Microsatellites In Geoffroeamentioning

confidence: 99%

Development and characterization of 11 microsatellite markers in a widespread Neotropical seasonally dry forest tree species, Geoffroea spinosa Jacq. (Leguminosae)

Naciri

Caetano

Prado

et al. 2005

Molecular Ecology Notes

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“…The use of molecular markers in population genetic studies of tree species has been shown to be effective. In M. urundeuva, previous studies were performed using isoenzyme markers [7,8], RAPD and cpDNA [9,10], AFLP [11,12] and more recently by microsatellite markers [1,3,[13][14][15]. In these last studies, nine microsatellite loci developed by Caetano et al [13] were used in the analysis of populations of M. urundeuva only from Paraguay and Argentina.…”

Section: Introductionmentioning

confidence: 99%

Development of microsatellite markers for Myracrodruon urundeuva (F.F. & M.F. Allemão), a highly endangered species from tropical forest based on next-generation sequencing

et al. 2017

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Myracrodruon urundeuva is a tree species of high economic importance due the strength and durability of its wood. Threatened of extinction in Brazil, it is present only in a few forest remnants, mostly in conservation units. Currently, there is little information on the genetic diversity of natural populations in Brazil and even less information about the genome of this species. Here, new species-specific microsatellite loci were developed based on next-generation sequencing (Illumina). More than 100,000 loci were identified in the run, with di- to hexanucleotides motifs. Of these, 20 loci were selected for validation in 30 individuals, with 15 successfully polymorphic loci detected. The number of alleles ranged among loci from 3 to 16, with an average of 7.73, expected (H ) and observed (H ) heterozygosity ranged from 0.246 to 0.902 and from 0.103 to 0.867, respectively. These results point out that these new set of markers has a great potential for use in population genetic studies for genetic conservation of the species.

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“…The reaction amplification of SSR loci were conducted to evaluate five loci described for M. urundeuva in Caetano et al (2005). Thus, the PCRs were composed of 1 ng of genomic DNA in a final volume of 6 mL containing: 200 µM of each dNTP, 2.5 mM MgCl 2 , 0.33 mM of each primer, ultrapure sterile water and 1x Go Taq Master Mix PROMEGA.…”

Section: Methodsmentioning

confidence: 99%

Mating system in Myracrodruon urundeuva (Anarcadiaceae): implications for conservation genetics

Gaino¹,

Moraes²,

Moreira³

et al. 2011

Rev. bras. Bot.

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-Mating system in Myracrodruon urundeuva (Anarcadiaceae): implications for conservation genetics. The aims of this study were to investigate the mating system of a fragmented population of the dioecious tropical tree Myracrodruon urundeuva Allemão, using five microsatellite loci and the mixed mating and correlated mating models. The study was conducted in the Estação Ecológica de Paulo de Farias (436 ha), where the population occupies about 142 ha. The mating system was estimated using 514 open-pollinated offspring, collected from 30 seed-trees. Estimates of the multilocus outcrossing rate confirm that the species is dioecious (t m = 1.0). Low levels of mating among relatives were detected in the population (1 -t s = 0.020). The estimate of paternity correlation (r p(m) ) indicated that offsprings were composed of mixtures of half-sibs and full-sibs, with the latter occurring at a low frequency (average of 0.148). The estimated coancestry coefficient within families (Θ = 0.147) was larger and the effective population size (N e(v) ) was lower (N e(v) = 2.98) than expected in progenies from panmictic populations (Θ = 0.125, N e(v) = 4, respectively). In terms of conservation, the results indicate that to retain an effective population size of 150, is necessary to collect seeds from at least 50 seed-trees.Key words -correlated matings, fragmentation, microsatellite, variance effective population size RESUMO -Sistema de reprodução em Myracrodruon urundeuva (Anarcadiaceae): implicações para a conservação genética. O objetivo deste estudo foi investigar o sistema de reprodução de uma população fragmentada da espécie arbórea dióica, Myracrodruon urundeuva Allemão, utilizando cinco locos microssatélites e os modelos mistos de reprodução e de cruzamentos correlacionados. O estudo foi conduzido na Estação Ecológica de Paulo de Faria (436 ha), onde a população ocupa uma área aproximada de 142 ha. Foram amostradas 514 progênies de polinização aberta coletadas de 30 árvores matrizes da população. A estimativa da taxa de cruzamento multilocus confirma que a espécie é dióica (t m = 1,0). Baixos níveis de cruzamentos entre parentes foram detectados na população (1 -t s = 0,020). A estimativa da correlação de paternidade (r p(m) ) indicou que as progênies são compostas por misturas de meios-irmãos e irmãos-completos, embora este último ocorra em menor proporção (média de 0,148). O coeficiente de coancestria dentro das progênies (Θ = 0,147) foi maior e o tamanho efetivo de variância (N e(v) = 2,98) foi menor do que o esperado em progênies de populações panmíticas (Θ = 0,125, N e(v) = 4, respectivamente). Em termos de conservação genética, os resultados indicam que para se obterem lotes de sementes com tamanho efetivo de referência de 150, é necessário coletar sementes de pelo menos 50 árvores matrizes.Palavras-chave -cruzamentos correlacionados, fragmentação, microssatélites, tamanho efetivo populacional 1.Parte da tese de doutorado da primeira autora,

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Identification of microsatellite markers in a neotropical seasonally dry forest tree, Astronium urundeuva (Anacardiaceae)

Cited by 12 publications

References 8 publications

Development and characterization of 11 microsatellite markers in a widespread Neotropical seasonally dry forest tree species, Geoffroea spinosa Jacq. (Leguminosae)

Development and characterization of 11 microsatellite markers in a widespread Neotropical seasonally dry forest tree species, Geoffroea spinosa Jacq. (Leguminosae)

Development of microsatellite markers for Myracrodruon urundeuva (F.F. & M.F. Allemão), a highly endangered species from tropical forest based on next-generation sequencing

Mating system in Myracrodruon urundeuva (Anarcadiaceae): implications for conservation genetics

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