Identification of Microsatellite (SSR) and RAPD Markers Linked to Rice Blast Disease Resistance gene in Rice (Oryza sativa L.)

C, Immanuel Selvaraj; Nagarajan, P.; Thiyagarajan, K.; Bharathi, M.; Rabindran, R.

doi:10.5897/ajb10.2573

Cited by 11 publications

(5 citation statements)

References 37 publications

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“…Several molecular marker systems such as random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), amplified fragment length polymorphism (AFLP), simple sequence repeat (SSR), inter-simple sequence repeat (ISSR), and single-strand conformation polymorphism (SSCP) have been used in the analysis of marker-trait association (Roy et al, 2006;Wang et al, 2010;Bandyopadhyay, 2011;Diaz et al, 2011;Immanuel et al, 2011;Kalivas et al, 2011;Milad et al, 2011;Nisar and Ghafoor, 2011;Yu et al, 2011). The association of these molecular markers with different traits related to disease and insect resistance has been established in several plants (Lefebvre and Chèvre, 1995;Obert et al, 2000;Shalini et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Association of RGA-SSCP markers with resistance to downy mildew and anthracnose in grapevines

Tantasawat¹,

Poolsawat²,

Prajongjai³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Downy mildew (Plasmopara viticola) and anthracnose (Sphaceloma ampelinum) are two major diseases that severely affect most grapevine (Vitis vinifera) cultivars grown commercially in Thailand. Progress of conventional breeding programs of grapevine for improved resistance to these diseases can be speeded up by selection of molecular markers associated with resistance traits. We evaluated the association between 13 resistance gene analog (RGA)-single-strand conformation polymorphism (SSCP) markers with resistance to downy mildew and anthracnose in 71 segregating progenies of seven cross combinations between susceptible cultivars and resistant lines. F 1 hybrids from each cross were assessed for resistance to downy mildew and anthracnose (isolates Nk4-1 and Rc2-1) under laboratory conditions. Association of resistance traits with RGA-SSCP markers was evaluated using simple linear regression analysis. Three RGA-SSCP markers were found to be significantly correlated with anthracnose resistance, whereas significant correlation with downy mildew resistance was observed for only one RGA-SSCP marker. These results demonstrate the usefulness of RGA-SSCP markers. Four candidate markers with significant associations to resistance to these two major diseases of grapevine were identified. However, these putative associations between markers and resistance need to be verified with larger segregating populations before they can be used for marker-assisted selection.

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Section: Introductionmentioning

confidence: 99%

Association of RGA-SSCP markers with resistance to downy mildew and anthracnose in grapevines

Tantasawat¹,

Poolsawat²,

Prajongjai³

et al. 2012

Genet. Mol. Res.

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“…The plant materials were evaluated based on the Uniform Blast Nursery (UBN) method [ 38 ] at the Hybrid Rice Evaluation Centre (HREC), Gudalur, Ooty, Tamil Nadu (11°30′ N latitude; 76°30′ E longitude and 1317.00 m above MSL elevation) from February to March 2020. This location is a natural hot spot for screening the leaf blast disease resistance since the blast incidence is recorded throughout the year and the incidence becomes severe during winter and rainy seasons [ 39 ]. All the plant materials, including the parents (CO 51 and 562-4), R gene(s)-advanced backcross bred rice lines and the susceptible check (CO 39), were sown in a 100 cm row with 10 cm gap between the successive rows with three replications.…”

Section: Methodsmentioning

confidence: 99%

Marker Assisted Introgression of Resistance Genes and Phenotypic Evaluation Enabled Identification of Durable and Broad-Spectrum Blast Resistance in Elite Rice Cultivar, CO 51

Thulasinathan

Ayyenar

Kambale

et al. 2023

Genes

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Across the globe, rice cultivation is seriously affected by blast disease, caused by Magnaporthe oryzae. This disease has caused heavy yield loss to farmers over the past few years. In this background, the most affordable and eco-friendly strategy is to introgress blast-resistant genes from donors into elite rice cultivars. However, it is not only challenging to evolve such resistance lines using conventional breeding approaches, but also a time-consuming process. Therefore, the marker-assisted introduction of resistance genes has been proposed as a rapid strategy to develop durable and broad-spectrum resistance in rice cultivars. The current study highlights the successful introgression of a blast resistance gene, i.e., Pi9, into CO 51, an elite rice cultivar which already has another resistance gene named Pi54. The presence of two blast resistance genes in the advanced backcross breeding materials (BC2F2:3) was confirmed in this study through a foreground selection method using functional markers such as NBS4 and Pi54MAS. The selected positive introgressed lines were further genotyped for background selection with 55 SSR markers that are specific to CO 51. Consequently, both Pi9 as well as Pi54 pyramided lines, with 82.7% to 88.1% of the recurrent parent genome recovery, were identified and the selected lines were evaluated under hotspot. The analysis outcomes found that both the lines possessed a high level of resistance against blast disease during the seedling stage itself. In addition to this, it was also noticed that the advanced breeding rice lines that carry Pi9 + Pi54 were effective in nature and exhibited a higher degree of resistance against blast disease compared to the lines that were introgressed with a single blast resistance gene. Thus, the current study demonstrates a rapid and a successful introgression and pyramiding of two blast resistance genes, with the help of markers, into a susceptible yet high-yielding elite rice cultivar within a short period of time. Those gene pyramided rice lines can be employed as donors to introgress the blast-resistant genes in other popular susceptible cultivars.

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“…Out of 8 markers which deviated from the Mendelian segregation ratio, 5 (9.61%) exhibited segregation distortion towards Moroberekan and 3 (5.77%) skewed towards White Ponni. The overall allele frequency for the 44 loci showed an overabundance of heterozygotic alleles [86].…”

Section: Mapping Survey Using Micro-satellite Markers (Ssr)mentioning

confidence: 96%

“…Among them, 52 SSR primer pairs produced unambiguous amplified products. Maximum number of markers were scored on chromosome 7 (10 primers) followed by chromosome 1 (9 primers) [86]. Multiple copy fragments were observed in some of the primers.…”

Section: Mapping Survey Using Micro-satellite Markers (Ssr)mentioning

confidence: 99%

Review: Rice Blast Disease

Shahriar

Imtiaz

Hossain

et al. 2020

ARRB

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Rice blast caused by Magnaporthe grisea is the major damaging disease in nearly all rice growing nations. Economically relevance with 60 percent of total population of world depending on rice as the main source of calories, may have destructive effects of the disease, however, this pathogen has developed into a pioneering model system for researching host-pathogen interactions. The disease outbreak depends on the weather and climatic conditions of the various regions. The disease's occurrence and symptoms vary from country to country. Susceptible cultivars cause huge rice production loss in yield. The principal cause of resistance breakdown in rice against rice blast disease is pathogenic variability. During sexual hybridization, pathogenic changes may provide evidence of pathogenic variation found at the asexual stage of the fungus. The virulent pathotypes cause severe disease incidence. Only through pathogenicity research the pathotypes can be determined using a collection of different rice varieties that are usually different carrying various resistance genes. Rice breeders now have a number of resistant genes however, most of the breeding programs emphasized upon monogenic resistance. Genetic heterogeneity of M. grisea should be taken into account when screening blast resistant rice genotypes through morphological analysis, pathogenicity and molecular characterization. Knowledge on the virulence of the rice blast and host resistant is essential for managing the disease. Cultivation of resistant varieties with chemical control is highly effective against blast pathogens.

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Identification of Microsatellite (SSR) and RAPD Markers Linked to Rice Blast Disease Resistance gene in Rice (Oryza sativa L.)

Cited by 11 publications

References 37 publications

Association of RGA-SSCP markers with resistance to downy mildew and anthracnose in grapevines

Association of RGA-SSCP markers with resistance to downy mildew and anthracnose in grapevines

Marker Assisted Introgression of Resistance Genes and Phenotypic Evaluation Enabled Identification of Durable and Broad-Spectrum Blast Resistance in Elite Rice Cultivar, CO 51

Review: Rice Blast Disease

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