Identification of miRNAs linked with the drought response of tef [Eragrostis tef (Zucc.) Trotter]

Martinelli, Federico; Cannarozzi, Gina; Balan, Bipin; Siegrist, Fredy; Weichert, Annett; Blösch, Regula; Tadele, Zerihun

doi:10.1016/j.jplph.2018.02.011

Cited by 16 publications

(17 citation statements)

References 67 publications

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“…Furthermore, the microRNA profiling using the next-generation sequencing of drought tolerant Tsedey variety and drought susceptible Alba landrace identified significantly modulated microRNAs in shoots and roots in response to drought (Martinelli et al 2018). Based on this, putative targets of these miRNAs were predicted.…”

Section: Omics Studies On Tefmentioning

confidence: 99%

Significance and prospects of an orphan crop tef

Chanyalew

Ferede

Damte

et al. 2019

Planta

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Section: Omics Studies On Tefmentioning

confidence: 99%

Significance and prospects of an orphan crop tef

Chanyalew

Ferede

Damte

et al. 2019

Planta

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“…Plant miRNAs can rapidly respond to different developmental and environmental signals and subsequently suppress the expression of their targets via mRNA cleavage or transcriptional inhibition [ 23 , 26 , 27 , 28 ]. These miRNA-target modules play significant roles in biological processes, such as signal transduction, nutrient transport, reproductive development, organ formation, and particularly, stress adaptation [ 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 ]. In crop plants, upon abiotic stress, miRNAs can quickly reprogram the expression of downstream genes that tightly regulate adaptive physiological traits, such as altered flowering timing and increased root water uptake.…”

Section: Introductionmentioning

confidence: 99%

Integrated Analysis of Small RNA, Transcriptome, and Degradome Sequencing Reveals the Water-Deficit and Heat Stress Response Network in Durum Wheat

Liu

Able

2020

IJMS

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Water-deficit and heat stress negatively impact crop production. Mechanisms underlying the response of durum wheat to such stresses are not well understood. With the new durum wheat genome assembly, we conducted the first multi-omics analysis with next-generation sequencing, providing a comprehensive description of the durum wheat small RNAome (sRNAome), mRNA transcriptome, and degradome. Single and combined water-deficit and heat stress were applied to stress-tolerant and -sensitive Australian genotypes to study their response at multiple time-points during reproduction. Analysis of 120 sRNA libraries identified 523 microRNAs (miRNAs), of which 55 were novel. Differentially expressed miRNAs (DEMs) were identified that had significantly altered expression subject to stress type, genotype, and time-point. Transcriptome sequencing identified 49,436 genes, with differentially expressed genes (DEGs) linked to processes associated with hormone homeostasis, photosynthesis, and signaling. With the first durum wheat degradome report, over 100,000 transcript target sites were characterized, and new miRNA-mRNA regulatory pairs were discovered. Integrated omics analysis identified key miRNA-mRNA modules (particularly, novel pairs of miRNAs and transcription factors) with antagonistic regulatory patterns subject to different stresses. GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis revealed significant roles in plant growth and stress adaptation. Our research provides novel and fundamental knowledge, at the whole-genome level, for transcriptional and post-transcriptional stress regulation in durum wheat.

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“…Several studies from recent years found tef accessions with increased tolerance to drought (Abraha et al 2016;Abraha et al 2017). In addition, our recent study identified 147 novel and unique putative miRNA precursors from tef which are associated to drought (Martinelli et al, 2018). Prediction of the targets of these miRNAs resulted in the identification of many transcription factors that may be potential targets for improvement of drought resistance.…”

Section: Terminal Drought Tolerance (Tdt)mentioning

confidence: 99%

“…Although orphan or indigenous crops often perform better than major world crops under extreme environmental conditions and fit to the socio-economic conditions of the areas in which they are grown, they are also subject to a number of environmental and policy-related constraints (Tadele, 2016(Tadele, , 2017. The Tef Improvement Project which is based at the University of Bern implements diverse genetic and genomic tools to develop tef lines with increased productivity (Cannarozzi, Chanyalew, et al, 2018;Cannarozzi et al, 2014;Cannarozzi, Weichert, et al, 2018;Jost et al, 2015). Tef [Eragrostis tef (Zucc.)…”

Section: Introductionmentioning

confidence: 99%

Identification of Drought Tolerant Mutant Lines of TEF [ERAGROSTIS TEF (ZUCC.) TROTTER]

Blösch

Rindisbacher

Plaza-Wüthrich

et al. 2019

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Introduction: Tef [Eragrostis tef (Zucc.) Trotter] is the major food crop in Ethiopia where it is annually cultivated on about 30% of the total area allocated to cereals. The productivity of tef is, however, significantly reduced by drought which can occur at either the early or the late developmental stage of the crop.Methodology: A total of 10,000 EMS (ethyl methane sulfonate) mutagenized M 2 tef populations were screened first for early and second for terminal drought tolerance. The performance of candidate lines was investigated using diverse agronomical and physiological parameters.Results and discussion: Several promising lines were obtained for both early and terminal drought tolerance. Among these, genotypes with outstanding performance were found for two drought tolerant tef lines targeting early drought tolerance (dtt2 and dtt13) and three terminal drought tolerant lines targeting terminal drought tolerance (tdt9, tdt15 and tdt19).Conclusions: Candidate lines for both early and terminal drought tolerance will play vital roles in developing drought tolerance in tef improvement. KEY WORDS: DROUGHT TOLERANCE, ERAGROSTIS TEF, ORPHAN CROP, MOISTURE SCARCITY, TEF afrika focus -Volume 32, Nr. 2 [ 26 ] REGULA BLÖSCH, ABIEL RINDISBACHER, SONIA PLAZA-WÜTHRICH, NORA RÖCKEL, ANNETT WEICHERT, GINA CANNAROZZI AND ZERIHUN TADELE afrika focus -Volume 32, Nr. 2 [ 36 ]REGULA BLÖSCH, ABIEL RINDISBACHER, SONIA PLAZA-WÜTHRICH, NORA RÖCKEL, ANNETT WEICHERT, GINA CANNAROZZI AND ZERIHUN TADELE

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Identification of miRNAs linked with the drought response of tef [Eragrostis tef (Zucc.) Trotter]

Cited by 16 publications

References 67 publications

Significance and prospects of an orphan crop tef

Significance and prospects of an orphan crop tef

Integrated Analysis of Small RNA, Transcriptome, and Degradome Sequencing Reveals the Water-Deficit and Heat Stress Response Network in Durum Wheat

Identification of Drought Tolerant Mutant Lines of TEF [ERAGROSTIS TEF (ZUCC.) TROTTER]

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