Identification of molecular marker associated with mungbean yellow mosaic virus resistance in mungbean [Vigna radiata (L.) Wilczek]

Dharajiya, Darshan T.; Ravindrababu, Y.

doi:10.1007/s42535-019-00063-y

Cited by 8 publications

(4 citation statements)

References 20 publications

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“…The mung bean yellow mosaic disease (MYMD) and bruchid infestation are major biotic stresses initially focused to develop genomic resources for forward breeding approaches. The RAPD marker OPP 07 895 was identified to be linked with MYMD resistance using bulk segregant analysis (Dharajiya and Ravindrababu 2019). Two QTLs, i.e., qMYMIV2 and qMYMIV7, with 31.42-37.60% and 29.07-47.36% PV, respectively, were reportedly linked to MYMD resistance (Alam et al 2014).…”

Section: Mung Beanmentioning

confidence: 99%

Forward Breeding for Efficient Selection

Bohar,

Dreisigacker,

Lindqvist-Kreuze

et al. 2024

Sustainability Sciences in Asia and Africa

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Global food security is the numero uno priority in the current global situation, threatened by a number of challenges catalyzed by accelerated climate change and population growth. Crop improvement coupled with the modern plant breeding approaches, such as genomic-assisted breeding, is a proven solution to meet the food security. One of the key mandates in the modern plant breeding program is to combine the power of genomic selection into the breeding pipeline employing a low-cost genotyping solution. Several SNP marker-based platforms are now available depending on the objectives and field of application; despite the R. Bohar (✉) CGIAR Excellence in Breeding (EiB), International Maize and Wheat Improvement Center (CIMMYT), c/o ICRISAT,

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Section: Mung Beanmentioning

confidence: 99%

Forward Breeding for Efficient Selection

Bohar,

Dreisigacker,

Lindqvist-Kreuze

et al. 2024

Sustainability Sciences in Asia and Africa

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“…Furthermore, to check that OPP 07895 RAPD marker was linked with MYMV resistance, co-segregation analysis was carried out in susceptible and resistant genotypes of mungbean. This molecular marker can also be helpful to identify Quantitative trait loci (QTL) associated with MYMV resistance (Dharajiya and Ravindrababu, 2019). Chen et al, (2013) described that three major quantitative trait loci (QTLs) linked to MYMV resistance genes and one major QTL against bruchid resistance have been identified.…”

Section: Stresses Of Mungbean Cropmentioning

confidence: 99%

“…The OPP 07895marker can be utilized for detecting MYMV-linked QTLs. This MYMV-linked OPP 07895RAPD marker could be converted into the SCAR marker for enhancing MAS effectiveness (Dharajiya and Ravindrababu, 2019). Protein markers are also helpful in differentiating resistant and susceptible genotypes but protein markers are not preferred over PCR-based markers because of their less yielding polymorphism capacity.…”

Section: Stresses Of Mungbean Cropmentioning

confidence: 99%

Perspectives of molecular marker-assisted breeding in mungbean (Vigna radiata L.) to develop resistance for mungbean yellow mosaic virus (MYMV) disease

Mukhtar

2023

PDC

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Mungbean (Vigna radiate L.) is a fast-growing warm seasonal pulse crop that has high nutritional and economical importance. It is grown for its seeds that have high protein content. It belongs to the family Fabaceae, widely distributed throughout the world mainly in Asian countries. In Pakistan, about 88% of mungbean production has occurred in the Punjab province. Various biotic and abiotic factors are responsible for a significant yield and productivity reduction in mungbean crop. While the major loss of mungbean production is mainly due to mungbean yellow mosaic virus (MYMV) which may lead to 5-100% yield loss. This disease is caused by geminiviruses, belong to begomovirus genus. To overcome this loss due to MYMV, host resistance is preferred over cultural and chemical control methods. This review summarizes the importance of begomoviruses to cause the disease, the role of vector whiteflies, implementation of various management strategies including host resistance and marker-assisted breeding. Molecular marker-assisted breeding for resistance or tolerance in mungbean against MYMV is an effective approach to combat this disease. Different molecular markers have been used to identify and characterize the mungbean resistance genotypes against MYMV. Several molecular markers have been identified through Bulked Segregant Analysis (BSA) which are linked to this disease.

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“…MAS is relatively more efficient and reliable than phenotypic screening as it involves DNA markers that eliminate environmental errors. Various quantitative trait loci (QTL) markers linked to Cercospora leaf spot disease and Mungbean yellow mosaic virus (MYMV) disease resistance in V. species have been documented [12,13]. Using molecular markers linked to the resistance genes is a promising approach in MAS.…”

Section: Introductionmentioning

confidence: 99%

Development of Molecular Marker Linked with Cercospora Leaf Spot (CLS) Disease Resistance in Vigna radiata, Cloning, and Expression for Evaluating Antifungal Activity against Cercospora canescens

Babar¹,

Ijaz²,

Haq³

et al. 2023

Phyton

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We developed a molecular marker for MAS of mungbean resistant varieties against CLS from the consensus sequence (MB-CLsRG) of identified RGAs (MB-ClsRCaG1 and MB-ClsRCaG2). The MB-CLsRG sequence-specific primer pair was used to screen Cercospora leaf spot (CLS) resistant varieties of mungbean in genomic analysis that showed congruency with phenotypic screening. Validation of molecular marker linkage with CLS resistance was performed using rtPCR in transcriptomic analysis. The sequenced PCR products showed 100% homology with MB-CLsRG sequence and putative disease resistance proteins that confirmed the linkage of molecular marker with CLS resistance in mungbean. The antifungal potential of MB-CLsRG gene encoding protein was assessed. The MB-CLsRG gene sequence was cloned in the E. coli expression vector for recombinant protein production. The recombinant protein was then investigated for its in vitro antifungal potential against Cercospora canescens. The in vitro investigation showed strong antifungal activity of recombinant protein as it restricted the growth of fungal mycelial mass. The results validated the linkage of developed marker with CLS-resistant mungbean varieties; therefore, it can be used to screen resistant varieties from a large population in MAS. Moreover, the recombinant protein of the MB-CLsRG gene sequence revealed antifungal potential, which proved the gene sequence could be suitable to use in transgenic plants technology to develop fungal-resistant transgenic crops.

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Identification of molecular marker associated with mungbean yellow mosaic virus resistance in mungbean [Vigna radiata (L.) Wilczek]

Cited by 8 publications

References 20 publications

Forward Breeding for Efficient Selection

Forward Breeding for Efficient Selection

Perspectives of molecular marker-assisted breeding in mungbean (Vigna radiata L.) to develop resistance for mungbean yellow mosaic virus (MYMV) disease

Development of Molecular Marker Linked with Cercospora Leaf Spot (CLS) Disease Resistance in Vigna radiata, Cloning, and Expression for Evaluating Antifungal Activity against Cercospora canescens

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