Identification of Motile Aeromonas Strains with the MicroScan WalkAway System in Conjunction with the Combo Negative Type 1S Panels

Abstract

This study was performed to compare the MicroScan WalkAway automated identification system in conjunction with the new MicroScan Combo Negative type 1S panels with conventional biochemical methods for identifying 85 environmental, clinical, and reference strains of eight Aeromonas species.

Evaluation of Two Miniaturized Systems, MicroScan W/A and BBL Crystal E/NF, for Identification of Clinical Isolates of Aeromonas spp

“…The poor accuracy of MicroScan in identifying Aeromonas isolates to the species level in our study contrasted with the results of Vivas et al (21). We tested isolates of all of the Aeromonas species, and only 19.3% of such isolates were correctly identified, while Vivas et al (21) tested isolates of eight species and correctly identified 78.8% of them.…”

“…If these results were correct, this would agree with those of Vivas et al (21), who stated that this is the most common clinical species. However, by using the 16S rDNA RFLP method, we found that A. hydrophila only represented 8.1% of the 490 clinical isolates tested (unpublished data).…”

“…The tendency of miniaturized biochemical identification systems to confuse Aeromonas isolates with Vibrio isolates noticed in our study was already known (2,19). In our case, MicroScan misidentified eight isolates (10.8%) as Vibrio fluvialis, results similar to those reported by Vivas et al (21). More troubling is the fact that BBL Crystal misidentified two isolates as Vibrio cholerae, which is of special relevance because of the clinical significance of this microorganism.…”

“…We tested isolates of all of the Aeromonas species, and only 19.3% of such isolates were correctly identified, while Vivas et al (21) tested isolates of eight species and correctly identified 78.8% of them. An explanation for this discrepancy could be the fact that those authors com- (4,6,10,14).…”

Modulation of the immune response to an Aeromonas hydrophila aroA live vaccine in rainbow trout: effect of culture media on the humoral immune response and complement consumption

“…vaccine for homologous and heterologous virulent strains in rainbow trout [32,33]. The aroA mutant shows typical A. hydrophila reactions using the MicroScan WalkAway automatic identification system after 48 h of incubation in Combo panels [34], and the interruption of the aroA gene in the mutant strain was confirmed by PCR assays according to Casco´n Soriano et al [35]. Stock cultures of the strain were maintained at ÿ80 (C with 30% glycerol, and were routinely grown in tryptic soy agar (TSA, Cultimed) at 22 (C.…”