Identification of multiple constituents in the traditional Chinese medicine formula Zhi‐zi‐chi decoction and rat plasma after oral administration by liquid chromatography coupled to quadrupole time‐of‐flight tandem mass spectrometry

Zhao, Xu; Long, Zhimin; Dai, Jinna; Bi, Kaishun; Chen, Xiaohui

doi:10.1002/rcm.6360

Cited by 36 publications

(39 citation statements)

References 17 publications

(45 reference statements)

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“…C18 is selected as a better chromatic shape is achieved at a more ideal retention time. This selection of C18 long column is consistent with several previous studies . In most of the cases, compounds with similar physical‐chemical characteristics should be selected as the internal standard for analysis; thus, isovanillin is selected for quantification purpose.…”

Section: Discussionsupporting

confidence: 72%

Comparative oral bioavailability of geniposide following oral administration of geniposide, Gardenia jasminoides Ellis fruits extracts and Gardenia herbal formulation in rats

Cheng¹,

Lin

Lin³

et al. 2014

Journal of Pharmacy and Pharmacology

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Section: Discussionsupporting

confidence: 72%

Comparative oral bioavailability of geniposide following oral administration of geniposide, Gardenia jasminoides Ellis fruits extracts and Gardenia herbal formulation in rats

Cheng¹,

Lin

Lin³

et al. 2014

Journal of Pharmacy and Pharmacology

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“…Moreover, the product ions at m/z 651, 489, 327 and 283 were similar to those of crocin‐2 after the successive losses of glucose (162 Da) and the carboxyl group (44 Da). Therefore, peaks 39 and 44 could be tentatively identified as crocin‐I and 13‐ trans ‐crocin‐I by comparison of their retention behavior with the literature (Zhao et al ., ; Xu et al ., ).…”

Section: Resultsmentioning

confidence: 99%

HPLC‐Q‐TOF‐MS/MS for analysis of major chemical constituents of Yinchen–Zhizi herb pair extract

Ling

et al. 2013

Biomedical Chromatography

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The Yinchen-Zhizi herb pair (YZHP) consists of Herba Artemisiae Scopariae (Yinchen in Chinese) and Fructus Gardeniae (Zhizi in Chinese), and is mainly used to treat icteric hepatitis, itching skin and eczema. However, the bioactive constituents responsible for the pharmacological effects of YZHP are still unclear to date. In this work, a rapid and sensitive method was established to comprehensively study the constituents in YZHP extract by HPLC-Q-TOF MS/MS. The analysis was performed on an HPLC system equipped with an Agilent poroshell 120 EC-C18 column (100 × 2.1 mm, 2.7 mm) working in a gradient elution program coupled to quadrupole-time-of-flight mass spectrometry operating in the negative ion mode. As a result, a total of 46 compounds including 17 from Herba Artemisiae Scopariae and 36 from Fructus Gardeniae were detected and tentatively identified in YZHP extract by comparing the retention time and mass spectrometry and retrieving the reference literature. More importantly, a series of constituents, such as many iridoid glycosides, were reported for the first time in this formula. The HPLC-Q-TOF MS/MS method was developed and utilized successfully to identify the major constituents in YZHP extract and would be helpful for further metabolism and pharmacology research on YZHP.

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“…High‐performance liquid chromatography (HPLC) coupled with high‐resolution mass spectrometry such as quadrupole time‐of‐flight tandem mass spectrometry (Q‐TOF‐MS/MS) has been proven to be an efficient tool for the identification of the chemical constituents and metabolites in complex matrices (Ren et al ., ; Zhao et al ., ; Zheng et al ., ). Therefore it has become an effective analytical technique in traditional Chinese medicine research.…”

Section: Introductionmentioning

confidence: 99%

Identification of chemical constituents and rat metabolites of Kangxianling granule by HPLC‐Q‐TOF‐MS/MS

Zheng

Fang

Cong

et al. 2015

Biomedical Chromatography

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A high-performance liquid chromatography coupled with quadrupole time-of-flight mass tandem mass spectrometry method was established to characterize the chemical constituents of Kangxianling granule (KXL), a traditional Chinese medicine formula, and the metabolic profile in rat urine and plasma after oral administration of KXL. A total of 27 compounds in KXL extract and 13 prototype compounds with 12 metabolites in rat urine and plasma were identified. Among the 27 detected compounds, 15 were identified by comparing the retention time and MS data with that of reference compounds and the other 12 compounds were tentatively assigned based on the MS data and reference literature. The main prototype components absorbed in rat were amygdalin, salvianolic acid B, tanshinones and anthraquinones. Hydroxylation, glucuronidation and sulfation were the principal metabolic pathways in rat. The results revealed that the 25 compounds identified in rat urine and plasma were the potential active ingredients of KXL, which provides helpful chemical information for further study of the pharmacology mechanism of KXL.

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Identification of multiple constituents in the traditional Chinese medicine formula Zhi‐zi‐chi decoction and rat plasma after oral administration by liquid chromatography coupled to quadrupole time‐of‐flight tandem mass spectrometry

Cited by 36 publications

References 17 publications

Comparative oral bioavailability of geniposide following oral administration of geniposide, Gardenia jasminoides Ellis fruits extracts and Gardenia herbal formulation in rats

Comparative oral bioavailability of geniposide following oral administration of geniposide, Gardenia jasminoides Ellis fruits extracts and Gardenia herbal formulation in rats

HPLC‐Q‐TOF‐MS/MS for analysis of major chemical constituents of Yinchen–Zhizi herb pair extract

Identification of chemical constituents and rat metabolites of Kangxianling granule by HPLC‐Q‐TOF‐MS/MS

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