Identification of murine T-cell epitopes on low-molecular-mass secretory proteins (CFP11, CFP17, and TB18.5) of Mycobacterium tuberculosis

Abstract

The low-molecular-mass secretory proteins of Mycobacterium tuberculosis have been shown to be major T-cell antigens during infection with the pathogenic bacterium. In this study, we determined murine T-cell epitopes on three low-molecular-mass proteins, CFP11 (Rv2433c), CFP17 (Rv1827), and TB18.5 (Rv0164) using DNA immunization of inbred mice. We analyzed interferon-gamma production from immune splenocytes in response to overlapping peptides covering these proteins. We identified two CD8+ T-cell epitopes on CFP11 and CFP17, one in BALB/c mice and the other in C57BL/6 mice, respectively. On TB18.5, we identified a CD8+ T-cell epitope in BALB/c mice and a CD4+ T-cell epitope in C57BL/6 mice. With the aid of computer algorithms, we could identify the minimal CD8+ T-cell epitopes. These T-cell epitopes are feasible for analysis of the role of antigen-specific T cells during M. tuberculosis infection.

Analysis of the Secretome and Identification of Novel Constituents from Culture Filtrate of Bacillus Calmette-Guérin Using High-resolution Mass Spectrometry

“…Although their exact functions remain to be elucidated, they appear to be major T-cell antigens during infection with pathogenic mycobacteria (55).…”

Evaluation of 5 Novel protein biomarkers for the rapid diagnosis of pulmonary and extra-pulmonary tuberculosis: preliminary results

“…It has been documented that expression levels as well as the characters of proteins expressed by the H37Ra, H37Rv, and clinical isolates are significantly different28. It is also important to mention that the diagnostic potential of the five recombinant proteins which we used in this study, has never been explored on such a scale for the diagnosis of tuberculosis29.…”

“…The antigen rSS4 is conserved hypothetical protein which stimulates T-cell response in the host. The rSS5 is a probable lipase/esterase LipN while rSS2 is an essential hypothetical protein29. The rSS3 is a phosphoglycerate kinase6 involved in the second phase of glycolysis and to best of our knowledge, none of these antigen has been used for serodiagnosis of tuberculosis in India.…”

The putative polyketide cyclase MSMEG_0129 fromMycobacterium smegmatis: purification, crystallization and X-ray crystallographic analysis

“…As a secreted protein (Malen et al, 2007), it also serves as a human T-cell antigen (Ireton et al, 2010) and possesses the ability to induce significant production of IFN-in human peripheral blood mononuclear cells (Lim et al, 2004;Sable et al, 2005). Furthermore, genetic immunization of inbred mice with plasmid DNA and re-stimulation with overlapping synthetic peptides covering this protein led to the discovery of murine T-cell epitopes on Rv0164 (Eweda et al, 2010). These experimental data suggest that Rv0164 is a candidate immunogen that has potential for vaccine development and serodiagnosis.…”