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The low-molecular-mass secretory proteins of Mycobacterium tuberculosis have been shown to be major T-cell antigens during infection with the pathogenic bacterium. In this study, we determined murine T-cell epitopes on three low-molecular-mass proteins, CFP11 (Rv2433c), CFP17 (Rv1827), and TB18.5 (Rv0164) using DNA immunization of inbred mice. We analyzed interferon-gamma production from immune splenocytes in response to overlapping peptides covering these proteins. We identified two CD8+ T-cell epitopes on CFP11 and CFP17, one in BALB/c mice and the other in C57BL/6 mice, respectively. On TB18.5, we identified a CD8+ T-cell epitope in BALB/c mice and a CD4+ T-cell epitope in C57BL/6 mice. With the aid of computer algorithms, we could identify the minimal CD8+ T-cell epitopes. These T-cell epitopes are feasible for analysis of the role of antigen-specific T cells during M. tuberculosis infection.

Mycobacterial DNA-binding protein 1 (MDP1) is a major protein antigen in mycobacteria and induces protective immunity against Mycobacterium tuberculosis infection in mice. In this study we determined murine T-cell epitopes on MDP1 with MDP1 DNA immunization in mice. We analyzed interferon-gamma production from the MDP1 DNA-immune splenocytes in response to 20-mer overlapping peptides covering MDP1 protein. We identified several CD4+ T-cell epitopes in three inbred mouse strains and one CD8+ T-cell epitope in C57BL/6 mice. These T-cell epitopes would be feasible for analysis of the role of MDP1-specific T-cells in protective immunity and for future vaccine design against M. tuberculosis infection.

Background:The unavailability of data on the long-term efficacy of COVID-19 vaccines and of effective specific treatment, in addition to the emergence of new COVID-19 variants, makes nonpharmaceutical measures a mainstay for preventing the spread of infection. The policy responses of governments to the pandemic should be integrated with public behavioral changes. This study examines public attitudes, practices and perceived quarantine competency in Saudi Arabia during the COVID-19 pandemic. Methods:A cross-sectional descriptive design using an online survey was employed.Convenience and snowball sampling were adopted, and 1022 valid responses were received.Findings: Most respondents demonstrated satisfactory practices, including wearing face masks (76.5%), keeping a safe distance (97.8%) and appropriate hand hygiene (99.2%). Fear of stigma (8.9%) and of financial consequences (0%) were minimal drivers of underreporting of symptoms among the respondents, whereas uncertainty of the relevance of symptoms (30.3%) was a main cause of not reporting to health authorities. Most participants (73.4%) abided by government instructions due to fear of infection rather than of legal penalties. Participants, especially females and Saudi citizens, reported a high level of perceived quarantine competence (12.9 ± 2.59).Spearman's rho correlation showed a significant positive association between wearing face masks and other precautionary practices, including keeping a safe distance (0.25, P < .01) and frequent handwashing (0.224, P < .01), which rules out the concern that wearing face masks would result in less adherence of the participants to other protective measures. Conclusion:Our findings suggest that COVID-19 awareness programs should focus more on males and non-Saudi populations. We also recommend the development of screening protocols and education programs for asthmatic patients in Saudi Arabia to avoid the consequences of confusing COVID-19 symptoms with those of asthma. What's known• COVID-19 is a newly emerging infectious respiratory disease that was declared a pandemic by the WHO on 11 March 2020. How to cite this article: Almarwani AM, Aljohani MS, Eweda G. Perceived quarantine competence, attitudes and practices among the public during the coronavirus pandemic: A