Identification of mycobacteria

Tsukamura, M

doi:10.1016/s0041-3879(67)80040-0

Cited by 151 publications

(129 citation statements)

References 19 publications

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“…Out of these 76, all strains were negative for 16 ( Table 2). The methods used for testing for the characters were described previously (19,38,39).…”

Section: Methodsmentioning

confidence: 99%

“…Absence of discontinuity between the species belonging to these complexes was reported by many investigators. Tsukamura (36) found in 1967 that M. tuberculosis and M. bovis could not be distinctly separated from each other by numerical classification. Stanford and Grange (32) reported that on a serological basis M. tuberculosis, M. bovis, M. africanum, and M. microti should be reduced to a single species M. tuberculosis, and M. nonchromogenicum, "M. novum" and M. terrae to a single species M. nonchromogenicum.…”

Section: Growth On Medium Containing 02\ P-emrnosalicylatementioning

confidence: 99%

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Numerical Classification of 280 Strains of Slowly Growing Mycobacteria

Tsukamura

1983

Microbiology and Immunology

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. nonchromogenicum, M. terrae, "M. novum," and M. triviale. Each of these clusters appeared to be reduced to a single species, M. avium Chester 1901 (7) and M. nonchromogenicum Tsukamura 1965 (33), respectively which have priority in each group. However, considering the wide use of the names of the species of these groups, Tsukamura (40) proposed that the names the M. avium complex and the M. nonchromogenicum complex be used for these clusters. Tsukamura et al (43,44) observed also that M. tuberculosis, 315

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“…Out of these 76, all strains were negative for 16 ( Table 2). The methods used for testing for the characters were described previously (19,38,39).…”

Section: Methodsmentioning

confidence: 99%

Section: Growth On Medium Containing 02\ P-emrnosalicylatementioning

confidence: 99%

Numerical Classification of 280 Strains of Slowly Growing Mycobacteria

Tsukamura

1983

Microbiology and Immunology

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“…Other properties of M. marinum include: allantoinase (Tsukamura, 1967); aL-fucosidase (Grange & McIntyre, 1979); pyrazinamidase (Wayne et al 1978); picric acid reduction (Kazda & Hampl, 1965); resistance to amithiozone, 10,g/ml;…”

Section: Identificationmentioning

confidence: 99%

Mycobacterium marinuminfections in man

Collins

Grange

Noble

et al. 1985

J. Hyg.

101

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Mycobacterium marinum, formerly known as ‘Mycobacterium balnei’, is a free-living organism which causes disease in fish and occasionally in man. When such infections occur in man they are usually associated with some aquatic activity, e.g. swimming, fishing, boating and keeping tropical fish. For this reason, the organism has been called a ‘leisure-time pathogen’ by Feldman, Long & David (1974) and the disease a ‘hobby hazard’ by Heineman, Spitzer & Pianphongsant (1972).

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“…thermoresistibile and M . chitae showed similar patterns, although these two species differ distinctly from each other in their phenetic characters (29). Four strains of M .…”

Section: Methodsmentioning

confidence: 82%

“…Most of the strains were identified through cooperative studies of the International Working Group on Mycobacterial Taxonomy (IWGMT) (11,18,35). Some strains were identified in this laboratory by methods described previously (29,30). The organisms were grown in Ogawa egg medium a t 37 C for 5 days (rapidly growing mycobacteria, M .…”

Section: Methodsmentioning

confidence: 99%

Differentiation Among Mycobacterial Species by Thin-Layer Chromatography

Tsukamura

Matsumoto

1975

International Journal of Systematic Bacteriology

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Thin-layer chromatography of the ethyl ether-ethanol-soluble fraction after incubation with ["S Imethionine is useful for differentiation among mycobacterial species. Scanning of the radioactivity on chromatograms showed various distribution patterns of radioactive spots, and the patterns were, in most cases, characteristic for the species. bouis showed significantly lower activity than the other mycobacteria. The present study was designed to develop the previous method into a more reliable one. MATERIALS AND METHODSThe strains used are listed in Table 1. Most of the strains were identified through cooperative studies of the International Working Group on Mycobacterial Taxonomy (IWGMT) (11,18,35). Some strains were identified in this laboratory by methods described previously (29, 30). The organisms were grown in Ogawa egg medium a t 37 C for 5 days (rapidly growing mycobacteria, M . phlei through M . flauescens in Table 1) or 14 days (slowly growing mycobacteria, M . kansasii through M . gordonae in Table 1). The cells were washed three times in saline and then weighed; 100-mg amounts (wet weight) of the cells were suspended in 2.0 ml of 0.067 M phosphate buffer solution (pH 7.1) containing 5 pCi of ~-[~~S ] m e t h i onine and 10 pg of sodium acetate per ml. The tubes containing the mixture were stdppered with cotton plugs and incubated a t 37 C for 20 h. After incubation, the cells were centrifuged a t 500 x g for 15 min and washed twice with 5.0 ml of chilled water. The cells were extracted twice with 2.0 ml of 10% trichloroacetic acid, each for 10 min. The acid extracts were separated from the cells by centrifugation and discarded. The cells were then extracted twice with 3.0 ml of ethyl ether-ethanol (l:l, vol/vol) for 10 min each. During the first 5 min of the extraction, the cells were mixed continuously with a glass stick a t room temperature (25 + 3 C); during the second 5 min, the tubes were allowed to stand in an incubator a t 37 C, during which time the cells sedimented. The extracts could then be separated easily by means of a pipette. The combined ethyl ether-ethanol-soluble fractions were labeled as "lipid fraction." The residue was dissolved in 2.0 ml of a 1% NaOH solution by heating a t 100 C in a water bath for 5 min.~-[~~S ] m e t h i o n i n e was a product of New England Nuclear Corp., Boston, Mass. (specific activity, 264Ci/mmol; radiochemical purity, > 93%; containing < 7.0% methionine sulfoxide, <0.2% methionine sulfone, and <0.2% homocysteic acid). Ethyl ether was a product of Katayama Chemical Company, Osaka, Japan (specific gravity, below 0.720; fraction [34 to 35 C], minimum 95% [by volume]; water, maximum 0.3%; nonvolatile, maximum 0.002%; acidity [CH,COOH], maximum 0.003%). Ethyl alcohol also was a product of Katayama Chemical Company, Osaka, Japan (bp, 78 C; specific gravity, below 0.797; nonvolatile, maximum 0.003%; acidity [CH,COOH], maximum 0.003%; heavy metals p b ] , maximum O.O002%; ketones [CH, -CO .CH,], maximum 0.002%; assay by specific gravity, minimum 99.46% [by volume...

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Identification of mycobacteria

Cited by 151 publications

References 19 publications

Numerical Classification of 280 Strains of Slowly Growing Mycobacteria

Numerical Classification of 280 Strains of Slowly Growing Mycobacteria

Mycobacterium marinuminfections in man

Differentiation Among Mycobacterial Species by Thin-Layer Chromatography

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