Identification of Neisseria gonorrhoeae using the Neisstrip rapid enzyme detection test.

Dealler, Stephen; Gough, K R; Campbell, L. Leon; Turner, Andrew M.; Hawkey, Peter M.

doi:10.1136/jcp.44.5.376

Cited by 5 publications

(2 citation statements)

References 4 publications

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“…This preformed hydrolase was previously considered to be almost universally present in N gonorrhoeae. [6][7][8][9] However, N gonorrhoeae strains lacking the expression of this enzyme as a result of point mutations or deletions in the encoding single-copy pip gene 7 10 have increasingly been identified, potentially resulting in incorrect, doubtful or delayed identification of the correct species. 7 9 11 Further, N meningitidis strains may also produce PIP.…”

mentioning

confidence: 99%

Global transmission of prolyliminopeptidase-negative Neisseria gonorrhoeae strains: implications for changes in diagnostic strategies

Unemo

Palmer

Blackmore

et al. 2006

Sexually Transmitted Infections

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Background: Species confirmation of Neisseria gonorrhoeae is commonly performed with biochemical kits, rely on the activity of the enzyme prolyliminopeptidase (PIP). This enzyme has previously been considered to be almost universally present in N gonorrhoeae. However, increasing numbers of N gonorrhoeae isolates lacking PIP activity have been identified. Objectives: To investigate the possibility of a widespread transmission of one or several N gonorrhoeae PIPnegative strains among several countries worldwide. Methods: PIP-negative N gonorrhoeae isolates cultured from 2001 to 2004 in Australia, New Zealand and Scotland were comprehensively characterised and compared with previous data from England and Denmark. All isolates were characterised by antibiotic susceptibility testing, serovar determination, pulsed-field gel electrophoresis (PFGE), opa-typing, sequencing of the entire porB gene and N gonorrhoeae multiantigen sequence typing (NG-MAST). Results: Most (83%) of the viable Australian isolates, and all the New Zealand and Scottish isolates were assigned serovar IB-4, with similar antibiograms, nearly identical porB1b gene sequences, identical (ST210) or highly related (ST292, ST1259) NG-MAST STs, and indistinguishable or related PFGE fingerprints as well as opa-types. The isolates showed characteristics indistinguishable or highly related to the previously described English and Danish outbreak strain. Conclusions: A comprehensive characterisation indicates a widespread dissemination, mainly among men who have sex with men (MSM), of indistinguishable and highly related genotypes that have evolved from a single N gonorrhoeae PIP-negative serovar IB-4 strain among several countries worldwide. An increased awareness of PIP-negative N gonorrhoeae strains is crucial and changes in the diagnostic strategies may need to be considered.

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confidence: 99%

Global transmission of prolyliminopeptidase-negative Neisseria gonorrhoeae strains: implications for changes in diagnostic strategies

Unemo

Palmer

Blackmore

et al. 2006

Sexually Transmitted Infections

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“…8 9 In a method evaluation carried out in 1991, only two out of 398 isolates of N gonorrhoeae were found to be Pip negative. 10 Interestingly a report from the Gonococcal Reference Unit in Bristol during 2001, raised awareness of this as 17 Pip negative N gonorrhoeae isolates had been forwarded to them over a 5 week period for confirmatory identification. 3 The emergence and apparent increase in the prevalence of these isolates suggests that they may have a selective advantage.…”

Section: Discussionmentioning

confidence: 99%

The prevalence of proline iminopeptidase negative Neisseria gonorrhoeae throughout England and Wales

Alexander¹

2006

Sexually Transmitted Infections

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Prolyl aminopeptidases: Reclassification, properties, production and industrial applications

Dong

Yang

Zhang

et al. 2022

Process Biochemistry

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Identification of Neisseria gonorrhoeae using the Neisstrip rapid enzyme detection test.

Cited by 5 publications

References 4 publications

Global transmission of prolyliminopeptidase-negative Neisseria gonorrhoeae strains: implications for changes in diagnostic strategies

Global transmission of prolyliminopeptidase-negative Neisseria gonorrhoeae strains: implications for changes in diagnostic strategies

The prevalence of proline iminopeptidase negative Neisseria gonorrhoeae throughout England and Wales

Prolyl aminopeptidases: Reclassification, properties, production and industrial applications

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