Identification of new cattle BoLA-DRB3 alleles by sequence-based typing

Takeshima, Shin‐nosuke; Ikegami, Mie; Morita, Mitsuo; Nakai, Yutaka; Aida, Yoko

doi:10.1007/s002510000293

Cited by 57 publications

(77 citation statements)

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“…This study was performed using the data obtained from a previous study (Yoshida et al. 2009) on BoLA‐DRB3 alleles identified by the PCR‐SBT method (Takeshima et al. 2001) from 170 Holstein cows (129 mastitis and 41 healthy cows).…”

Section: Methodsmentioning

confidence: 99%

Association of the amino acid motifs of BoLA‐DRB3 alleles with mastitis pathogens in Japanese Holstein cows

Yoshida¹,

Mukoyama

Furuta³

et al. 2009

Animal Science Journal

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The association of the polymorphism of bovine leukocyte antigen (BoLA-DRB3) genes, identified by the polymerase chain reaction sequence-based typing (PCR-SBT) method, with resistance and susceptibility to mastitis caused by Streptococci, coagulase-negative Staphylococci, Escherichia coli and Staphylococcus aureus was investigated. Blood samples for DNA extraction were collected from 170 Holstein cows (129 mastitis and 41 healthy cows) from 5 districts in Chiba prefecture, Japan. Susceptibility or resistance to the mastitis-causing pathogens was thought to vary by the presence of amino acid substitutions at the 9, 11, 13, and 30 positions. DRB3*0101 and DRB3*1501 had amino acid motifs of Glu(9), Ser(11), Ser(13), and Tyr(30), and they were considered to have susceptibility to all 4 mastitis pathogens. In contrast, DRB3*1101 and DRB3*1401 had amino acid motifs of Gln(9), His(11), Gly(13), and His(30) in these positions, and they also had Val(86), so these alleles were considered to have resistance to Streptococcal and coagulase-negative Staphylococcal mastitis. However, in the case of Escherichia coli mastitis, amino acid substitutions at the 9, 11, 13, and 30 positions had little effect, but rather substitutions at the 47, 67 positions of pocket 7, and at the 71, 74 positions of pocket 4, Tyr(47), Ile(67), Ala(71), and Ala(74), were associated with resistance. This motif was present in DRB3*1201.

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Section: Methodsmentioning

confidence: 99%

Association of the amino acid motifs of BoLA‐DRB3 alleles with mastitis pathogens in Japanese Holstein cows

Yoshida¹,

Mukoyama

Furuta³

et al. 2009

Animal Science Journal

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“…1997). Previously, we were the first to perform direct sequencing of exon 2 of DRB3 gene from 471 individuals in four different Japanese populations of cattle (201 Japanese Black, 101 Holstein, 100 Japanese Shorthorn, and 69 Jersey cattle) using our new method for sequence‐based typing (Takeshima et al . 2001, 2002, 2003).…”

Section: Bola Class II Genes and Moleculesmentioning

confidence: 99%

“…The determination of genomic sequence permitted the application of PCR technology to the identification of sequence variation in coding exon of BoLA gene (van Eijk et al . 1992; Takeshima et al . 2001).…”

Section: Introductionmentioning

confidence: 99%

Structure, function and disease susceptibility of the bovine major histocompatibility complex

Takeshima¹,

Aida²

2006

Animal Science Journal

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103

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The major histocompatibility complex (MHC) of cattle is known as the bovine leukocyte antigen (BoLA) and is located on chromosome 23. BoLA has been linked to variation in resistance to disease including bovine leukemia virus‐induced lymphoma and mastitis. Moreover, BoLA appears to influence other traits such as milk yield, growth and reproduction, which are not often measured in humans, and variations in individual immune response to antigen. The BoLA appears to be organized in a similar way to the MHC region in humans, but there are notable differences. A major rearrangement within the class II region has led to the division of the BoLA into two distinct subregions of chromosome 23 separated by about a third of the chromosome’s length. The class IIa subregion contains functionally expressed DR and DQ genes, while the class IIb subregion contains the genes of undefined status such as DYA, DYB, DMA, DMB, DOB, DOA, TAP1, TAP2, LAP2 and LMP7. In addition, one pair of human class II genes (DP) does not appear to have an equivalent in cattle, and there is one pair of DY genes that seem to be found only cattle, sheep and goats. In humans, three classical, polymorphic class I genes (HLA‐A, ‐B and ‐C ) are each present on all haplotypes. However, in cattle, none of the four (or more) classical class‐I genes identified are consistently expressed, and haplotypes differ from one to another in both the gene number and composition. These variations in both class I and II are likely to play an important role in cattle immune responses. This review summarizes current knowledge of the structural and functional features and disease association of BoLA genes.

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“…For DNA typing for OLA-DRB1, 52 Suffolk sheep were also obtained from the JAPAN LAMB Co., Ltd. Peripheral blood mononuclear cells (PBMC) were isolated from blood as described by Miyasaka and Trnka (27) and samples of genomic DNA were obtained from whole blood as described by Takeshima et al (44,45). Four animals were experimentally inoculated with 1 ml of whole blood from BLV-infected cow, and two sheep were inoculated with phosphate-buffered saline (PBS) as control.…”

Section: Animals Cells Extraction Of Dna and Inoculation With Blvmentioning

confidence: 99%

The Influence of Ovine MHC Class IIDRB1Alleles on Immune Response in Bovine Leukemia Virus Infection

Konnai

Takeshima

Tajima

et al. 2003

Microbiology and Immunology

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We have reported previously that the alleles of the ovine leukocyte antigen (OLA)‐DRB1 gene that encode the Arg‐Lys (RK) motif and the Ser‐Arg (SR) motif at positions β70/71 of the OLA‐DRβ1 domain are associated with resistance and susceptibility, respectively, to development of bovine leukemia virus (BLV)‐induced ovine lymphoma. Here, to investigate the different immune response in sheep that carried alleles associated with resistance and susceptible for 30 weeks after infection with BLV, we selected sheep that had the RK/RK or SR/SR genotype among the 52 sheep analyzed by polymerase chain reaction‐restriction fragment length polymorphism and DNA sequencing of PCR product for the OLA‐DRB1 exon 2 and infected them with BLV. Although the number of BLV‐infected cells and virus titer had been maintaining low levels throughout the experimental period, the sheep with the RK/RK genotype could induce expansion of CD5− B‐cells and rapid production of neutralizing antibody in the early phase of infection. The level of incorporation of [3H]thymidine by peripheral blood mononuclear cells from the sheep with RK/RK genotype gave a strong response to BLV virion antigen and synthetic antigenic peptides that corresponded to T‐helper epitope of the BLV envelope glycoprotein gp51. In contrast, the sheep with SR/SR genotype showed a strong response to BLV virion antigen and synthetic antigenic peptides that corresponded to T‐cytotoxic and B‐cell epitopes. In such cases, the animals with the RK/RK strongly expressed IFN‐7, the animals with SR/SR genotype strongly expressed IL‐2. To determine the proliferating cells, we tried a blocking assay with monoclonal antibodies such as anti‐CD4, ‐CD8 and ‐DR molecule. We found that these proliferating cells were MHC‐restricted CD4+ T‐cells.

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Identification of new cattle BoLA-DRB3 alleles by sequence-based typing

Cited by 57 publications

References 0 publications

Association of the amino acid motifs of BoLA‐DRB3 alleles with mastitis pathogens in Japanese Holstein cows

Association of the amino acid motifs of BoLA‐DRB3 alleles with mastitis pathogens in Japanese Holstein cows

Structure, function and disease susceptibility of the bovine major histocompatibility complex

The Influence of Ovine MHC Class IIDRB1Alleles on Immune Response in Bovine Leukemia Virus Infection

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