Identification of new modes of Dd-STATa regulation of gene expression in Dictyostelium by in situ hybridisation

Shimada, Nao; Maeda, Mineko; Urushihara, Hideko; Kawata, Takefumi

doi:10.1387/ijdb.041862ns

Cited by 18 publications

(43 citation statements)

References 16 publications

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“…Reduced SSK395 gene expression in Dd-STATa-null mutant at the late slug stage but not at the early stages Interestingly, although expression at the tipped finger stage is detectable in the Dd-STATa null mutant, the transcript is hardly detectable in the mutant at the late slug stage (Shimada et al, 2004a). Therefore, only later expression appears to be positively regulated by Dd-STATa.…”

Section: Prestalk Cell-specific Expression Of the Ssk395 Genementioning

confidence: 89%

“…In a previous study, we identified 13 prestalk-specific genes whose expression is down regulated in the Dd-STATa-null mutant (Shimada et al, 2004a). One of those is an EST clone SSK395, which is detectable at the tipped finger stage by in situ hybridization (Shimada et al,2004a).…”

Section: Prestalk Cell-specific Expression Of the Ssk395 Genementioning

confidence: 99%

“…One of those is an EST clone SSK395, which is detectable at the tipped finger stage by in situ hybridization (Shimada et al,2004a). We focused on the function of the gene encoding SSK395, because the expression pattern dramatically changes as development proceeds.…”

Section: Prestalk Cell-specific Expression Of the Ssk395 Genementioning

confidence: 99%

“…The expression level becomes elevated again at the slug stage and it is observed throughout the entire prestalk region. Once culmination begins, expression in pstA cells disappears and localisation of the transcript then gradually shifts to the upper cup cells and the stalk tube entrance in mid culminants (Shimada et al, 2004a).…”

Section: Prestalk Cell-specific Expression Of the Ssk395 Genementioning

confidence: 99%

“…These were analysed by microarrays and in situ hybridization (Maeda et al, 2003) and we identified 13 candidate genes (Shimada et al, 2004a). We previously analysed one of those genes, the SSK395(ahhA) gene.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of a homologue of the adducin head gene which is a potential target for the Dictyostelium STAT protein Dd-STATa

Aoshima¹,

Hiraoka²,

Shimada³

et al. 2006

Int. J. Dev. Biol.

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A Dd-STATa-null mutant, which is defective in expression of a Dictyostelium homologue of the metazoan STAT (signal transducers and activators of transcription) proteins, fails to culminate and this phenotype correlates with the loss of expression of various prestalk (pst) genes. An EST clone, SSK395, encodes a close homologue of the adducin amino-terminal head domain and harbors a putative actin-binding domain. We fused promoter fragments of the cognate gene, ahhA (adducin head homologue A), to a lacZ reporter and determined their expression pattern. The proximal promoter region is necessary for the expression of ahhA at an early (pre-aggregative) stage of development and this expression is Dd-STATa independent. The distal promoter region is necessary for expression at later stages of development in pstA cells, of the slug and in upper cup and pstAB cells during culmination. The distal region is partly Dd-STATadependent. The ahhA-null mutant develops almost normally until culmination, but it forms slanting culminants that tend to collapse on to the substratum. The mutant also occasionally forms'fruiting bodies with swollen papillae and with constrictions in the prestalk region. The AhhA protein localizes to the stalk tube entrance and also to the upper cup cells and in cells at or near to the constricted region where an F-actin ring is localized. These findings suggest that DdSTATa regulates culmination and may be necessary for straight downward elongation of the stalk, via the putative actin-binding protein AhhA.

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Section: Prestalk Cell-specific Expression Of the Ssk395 Genementioning

confidence: 89%

Section: Prestalk Cell-specific Expression Of the Ssk395 Genementioning

confidence: 99%

Section: Prestalk Cell-specific Expression Of the Ssk395 Genementioning

confidence: 99%

Section: Prestalk Cell-specific Expression Of the Ssk395 Genementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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