Identification of new self-incompatibility alleles in sweet cherry (Prunus avium L.) and clarification of incompatibility groups by PCR and sequencing analysis

Wiersma, Paul A.; Wu, Zhi‐Ying; Zhou, Lin; Hampson, C.; Kappel, F.

doi:10.1007/s001220051700

Cited by 98 publications

(102 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2A). Using this system, many researchers determined S-genotypes of local cultivars and assigned new S-alleles (Choi et al, 2002;Wiersma et al, 2001;Yamane et al, 2000aYamane et al, , 2000b. A review of the sweet cherry S-allele literature revealed that potentially similar S-alleles had been assigned different nomenclatures ).…”

Section: Subgenus Cerasus (Cherries) 1) Identification Of S-rnase Allmentioning

confidence: 99%

Molecular Basis of Self-(in)compatibility and Current Status of S-genotyping in Rosaceous Fruit Trees

Yamane

Tao

2009

J. Japan. Soc. Hort. Sci.

View full text Add to dashboard Cite

Most rosaceous fruit tree species exhibit gametophytic self-incompatibility (GSI). During the last decade, much progress has been made in identifying and characterizing the S-locus genes that control the specificity of the selfincompatible (SI) reaction in the Rosaceae. The pistil and pollen determinants of S-specificity in the Rosaceae are a ribonuclease and an F-box protein, respectively. From allele-specific sequence polymorphism of S-specificity genes, molecular S-genotyping systems have been established in various rosaceous fruit tree species. Molecular characterization of S-determinants in self-compatible (SC) cultivars has revealed defects in the S-determinants that cause SC and enabled the development of molecular markers for SC. Marker-assisted selection of SC offspring is now being successfully incorporated into breeding programs. In this paper, we summarize the current knowledge of the molecular basis of self-(in)compatibility, S-genotyping methods, and molecular markers for SC in rosaceous fruit tree species.

show abstract

Section: Subgenus Cerasus (Cherries) 1) Identification Of S-rnase Allmentioning

confidence: 99%

Molecular Basis of Self-(in)compatibility and Current Status of S-genotyping in Rosaceous Fruit Trees

Yamane

Tao

2009

J. Japan. Soc. Hort. Sci.

View full text Add to dashboard Cite

show abstract

“…In the past, several P. avium S-alleles were identified as a result of cross pollination experiments and progeny testing in sweet cherry (Crane, Brown 1937) and later correlated with stylar ribonucleases (Bošković, Tobutt 1996). These S-RNases were also characterized at the molecular level (Tao et al 1999;Sonneveld et al 2001Sonneveld et al , 2003Wiersma et al 2001;Wunsch, Hormaza 2004;De Cuyper et al 2005).…”

mentioning

confidence: 99%

“…To identify the S-RNase alleles in sweet and wild cherry, polymerase chain reaction techniques are employed along with primers designed within conserved regions flanking each of the S-RNase introns (Tao et al 1999;Wiersma et al 2001;Sonneveld et al 2003;Kato, Mukai 2004;De Cuyper et al 2005;Schueler et al 2006). The application of fluorescently labelled primers for the intron associated with the 5' untranslated region (UTR) of the SFB gene and for the first intron of the S-RNase gene was also demonstrated for S-haplotype identification in P. avium (Sonneveld et al 2006;Vaughan et al 2006).…”

mentioning

confidence: 99%

Allele-specific PCR detection of sweet cherry self-incompatibility alleles S3, S4 and S9 using consensus and allele-specific primers in the Czech Republic

Sharma

Sedlák

Zeka

et al. 2014

Hort. Sci. (Prague)

View full text Add to dashboard Cite

Sharma K., Sedlák P., Zeka D., Vejl P., Soukup J., 2014. Allele-specific PCR detection of sweet cherry selfincompatibility alleles S 3 , S 4 and S 9 using consensus and allele-specific primers in the Czech Republic. Hort. Sci. (Prague), 41: 153-159.Prunus avium species of the Rosaceae family exhibit gametophytic self-incompatibility. Determination of the selfincompatibility genotype of individuals is essential for genetic studies and the development of informed management strategies. The PCR-based detection of S-allele helps to promote and speed up traditional breeding activity and hence molecular analysis of the perspective genotypes has become more intensive in all cherry growing countries. The alleles S 3 , S 4 and S 9 from 34 accessions of Czech collections were determined using the polymerase chain reaction (PCR) method. Initially, DNA extracts were amplified with consensus primers that amplify across the first, second, or both introns of the S-ribonuclease gene which shows a considerable length polymorphism. The new allele specific primers were designed with the goal to overcome some occurring difficulties in the detection of expected alleles by previously published allele specific primers. S-alleles fragments of standard cultivars used in this study were PCR amplified, sequenced to validate the designed primers. The study demonstrates the advantage of newly designed primers application in testing of sweet cherry genotypes. Keywords: Prunus avium; S-alleles; allele-specific primers; consensus primersSweet cherries Prunus avium (L.) are usually diploid (2n = 2× = 16) trees (Webster 1996;Iezzoni 2008) which are characterized by mighty growth and a pyramidal tree top. Gametophytic self-incompatibility (GSI) in cherry is controlled by the multi-allelic S-locus, which prevents self-fertilization (de Nettancourt 2001;Franklin-Tong, Franklin 2003). The S-genotype is agronomically important because cultivars with the same S-genotype are cross incompatible.The stylar component of GSI was determined to be a ribonuclease (S-RNase) gene in the Rosaceae (Bošković, Tobutt 1996;Sassa et al. 1996). In the past, several P. avium S-alleles were identified as a result of cross pollination experiments and progeny testing in sweet cherry (Crane, Brown 1937) and later correlated with stylar ribonucleases (Bošković, Tobutt 1996). These S-RNases were also characterized at the molecular level (Tao et al. 1999;

show abstract

“…The self-incompatibility reaction is triggered when the S gene is expressed in the pollen (SFB gene) and pistil (S-RNasa gene), which stops pollen tube growth. Knowledge of the genetic and molecular bases of the self-incompatible reaction has allowed the application of molecular techniques such as PCR amplification of S alleles with consensus or specific primers that amplify the two introns, which are part of the sequence of the S-RNasa gene (Tao et al, 1999;Tobutt et al, 2001;Wiersma et al, 2001;Sonneveld et al, 2001;Wünsch and Hormaza, 2004;Sonneveld et al, 2006), or amplify regions of the SFB gene (Sonneveld et al, 2005). The peach is self-fertile; in contrast, most cultivated Prunus species, such as cherry, almond, plums, and apricot, are self-infertile and therefore self-incompatible.…”

Section: Molecular Markersmentioning

confidence: 99%

Breeding rootstocks for Prunus species: Advances in genetic and genomics of peach and cherry as a model

Guajardo¹,

Hinrichsen

Muñoz

2015

Chilean J. Agric. Res.

View full text Add to dashboard Cite

Prunus rootstock is an important choice in optimizing productivity of grafted cultivars. Nevertheless, many Prunus rootstocks are notoriously intolerant to hypoxia which is caused by waterlogging and/or heavy soils. There is no available information to help select Prunus rootstocks that are tolerant to stress conditions such as root hypoxia caused by excess moisture. Information from genetic maps has demonstrated a high level of synteny among Prunus species, and this suggests that they all share a similar genomic structure. It should be possible to identify the genetic determinants involved in tolerance to hypoxia and other traits in Prunus rootstocks by applying methods to identify regions of the genome involved in the expression of important traits; these have been developed mainly in peach which is the model species for the genus. Molecular markers that are tightly linked to major genes would be useful in marker-assisted selection (MAS) to optimize new rootstock selection. This article provides insight on the advances in the development of molecular markers, genetic maps, and gene identification in Prunus, mainly in peach; the aim is to provide a general approach for identifying the genetic determinants of hypoxia stress in rootstocks.

show abstract

Identification of new self-incompatibility alleles in sweet cherry (Prunus avium L.) and clarification of incompatibility groups by PCR and sequencing analysis

Cited by 98 publications

References 19 publications

Molecular Basis of Self-(in)compatibility and Current Status of S-genotyping in Rosaceous Fruit Trees

Molecular Basis of Self-(in)compatibility and Current Status of S-genotyping in Rosaceous Fruit Trees

Allele-specific PCR detection of sweet cherry self-incompatibility alleles S<sub>3</sub>, S<sub>4</sub> and S<sub>9</sub> using consensus and allele-specific primers in the Czech Republic

Breeding rootstocks for Prunus species: Advances in genetic and genomics of peach and cherry as a model

Contact Info

Product

Resources

About