Identification of new sources of resistance to resistance-breaking isolates of tomato spotted wilt virus

Kabaş, Aylin; Fidan, Hakan; Demirelli, M. Batuhan

doi:10.1016/j.sjbs.2021.02.053

Cited by 13 publications

(7 citation statements)

References 41 publications

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“…In Turkey, PMMoV has been identified on pepper several times since 2013 (Caglar et al 2013). In a study conducted in Antalya, it was reported that genes that provide monogenic resistance to TSWV such as Tsw and Sw-5 become inactive at high temperatures and the state of resistance disappears (Kabas et al 2021).…”

Section: Discussionmentioning

confidence: 99%

Investigation of activity of Tobamovirus in pepper plants containing L4 resistance gene

Fidan¹,

GONBADİ

Sarıkaya³

et al. 2022

Mediterranean Agricultural Sciences

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Pepper mild mottle virus (PMMoV) is a plant virus belonging to the Virgaviridae family; it significantly reduces pepper yield production worldwide. The PMMoV is spread by contaminated seeds and there is no chemical treatment available. Therefore, resistant pepper varieties containing the L4 gene are recommended for the management of PMMoV. A considerable amount of evidence suggests that the L4 gene confers resistance to PMMoV in pepper. The aim of the project is to confirm the status of the L4 gene for resistance to PMMoV in pepper varieties, several inoculations were performed on pepper plants containing L3, L4 resistant genes and susceptible pepper plants without the resistance genes. The L4 resistant plants produced mottling, mosaic, leaf curl, stem necrosis symptoms in the tested pepper plants but there was no amplicon observed with specific primers of PMMoV in RT-PCR analyses. To determine if the L3 and L4 genes are controlling resistance to PMMoV, RT-PCR analyzes were conducted using PMMoV and Tomato brown rugose fruit virus (ToBRFV) where both viruses belong to the same family. The molecular studies revealed that the L4 gene controls resistance mechanisms to PMMoV but it is not able to govern Tobamovirus, ToBRFV. We showed that pepper plants harboring the L3 and L4 gene have the ability to precisely control the mechanism of resistance to PMMoV compared to pepper plants carrying only the L3 gene. A complete genome sequence of PMMoV was obtained and submitted to Genbank with MW523006 accessıon number in the NCBI system.

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Section: Discussionmentioning

confidence: 99%

Investigation of activity of Tobamovirus in pepper plants containing L4 resistance gene

Fidan¹,

GONBADİ

Sarıkaya³

et al. 2022

Mediterranean Agricultural Sciences

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“…Although new sources of resistance to RB strains have been recently reported in some wild tomato accessions (Kabaş et al 2021), at this time, cultivars carrying these resistance genes are not yet available, and thus, it is advisable…”

Section: Morphological Assessmentmentioning

confidence: 99%

Pyramiding disease resistance in tomato by duplex PCR targeting resistance genes and exploiting gene linkage

Parrella

Troiano

2022

Crop Breed. Appl. Biotechnol.

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Tomato is the second most important vegetable crop in the world after potato. Its production is hindered by fungal and viral diseases. Control of these diseases requires a significant investment that represents up to 40% of the production costs. Marker-assisted gene pyramiding is a powerful breeding tool which allow the rapid constitution of breeding lines with broad spectrum of resistance against diseases. Nevertheless, the accuracy and rapidity of introgression of genes of interest are some of the most critical barriers to successful application. In this paper, we use a simple and rapid method that combines duplex PCR for two important virus resistance genes (i.e., Sw-5 and Tm-2 2 ) and uses the linkage between genes Tm-2 2 and Forl to speed the introgression of these resistance genes into a "Pomodoro di Sorrento" tomato line. The method was used through six generations of backcrossing followed by three generations of selfing and resulted in a homozygous resistant line.

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“…Mechanical inoculation is a simple and effective method for conducting genetic re search on resistance to TSWV. A large number of plants can be quickly screened simulta neously with several isolates for the identification of resistant resources [35,36]. An inoc ulation buffer containing potassium phosphate buffer (0.1 M, pH 7.0), sodium sulphite (0.2% w/v), and polyvinyl pyrolidon (2% w/v) was used.…”

Section: Mechanical Inoculationmentioning

confidence: 99%

Natural Resources Resistance to Tomato Spotted Wilt Virus (TSWV) in Tomato (Solanum lycopersicum)

Zhang

Islam

et al. 2021

IJMS

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Tomato spotted wilt virus (TSWV) is one of the most destructive diseases affecting tomato (Solanum lycopersicum) cultivation and production worldwide. As defenses against TSWV, natural resistance genes have been identified in tomato, including Sw-1a, Sw-1b, sw-2, sw-3, sw-4, Sw-5, Sw-6, and Sw-7. However, only Sw-5 exhibits a high level of resistance to the TSWV. Thus, it has been cloned and widely used in the breeding of tomato with resistance to the disease. Due to the global spread of TSWV, resistance induced by Sw-5 decreases over time and can be overcome or broken by a high concentration of TSWV. How to utilize other resistance genes and identify novel resistance resources are key approaches for breeding tomato with resistance to TSWV. In this review, the characteristics of natural resistance genes, natural resistance resources, molecular markers for assisted selection, and methods for evaluating resistance to TSWV are summarized. The aim is to provide a theoretical basis for identifying, utilizing resistance genes, and developing tomato varieties that are resistant to TSWV.

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Identification of new sources of resistance to resistance-breaking isolates of tomato spotted wilt virus

Cited by 13 publications

References 41 publications

Investigation of activity of Tobamovirus in pepper plants containing L4 resistance gene

Investigation of activity of Tobamovirus in pepper plants containing L4 resistance gene

Pyramiding disease resistance in tomato by duplex PCR targeting resistance genes and exploiting gene linkage

Natural Resources Resistance to Tomato Spotted Wilt Virus (TSWV) in Tomato (Solanum lycopersicum)

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