Identification of new tetrahydroxylated metabolites of Polycyclic Aromatic Hydrocarbons in hair as biomarkers of exposure and signature of DNA adduct levels

Grova, Nathalie; Antignac, Jean‐Philippe; Hardy, Emilie; Monteau, Fabrice; Pouponneau, Karine; Bizec, Bruno Le; Appenzeller, Brice

doi:10.1016/j.aca.2017.10.002

Cited by 15 publications

(2 citation statements)

References 29 publications

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“…The hair samples went through a decontamination procedure prior to analysis [57]. After hair pulverization, hydrolysis, and extraction, the extract was analyzed by GC-MS/MS or LC-MS/MS as described previously [11,[58][59][60]. The limits of detection for PAHs and metabolites of PAHs quantified were assessed as described previously [11].…”

Section: Analysis Of Pahs In Hair Samplesmentioning

confidence: 99%

Changes of the human skin microbiota upon chronic exposure to polycyclic aromatic hydrocarbon pollutants

Leung¹,

Tong²,

Bastien³

et al. 2020

Microbiome

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Background: Polycyclic aromatic hydrocarbons (PAHs) are of environmental and public health concerns and contribute to adverse skin attributes such as premature skin aging and pigmentary disorder. However, little information is available on the potential roles of chronic urban PAH pollutant exposure on the cutaneous microbiota. Given the roles of the skin microbiota have on healthy and undesirable skin phenotypes and the relationships between PAHs and skin properties, we hypothesize that exposure of PAHs may be associated with changes in the cutaneous microbiota. In this study, the skin microbiota of over two hundred Chinese individuals from two cities in China with varying exposure levels of PAHs were characterized by bacterial and fungal amplicon and shotgun metagenomics sequencing. Results: Skin site and city were strong parameters in changing microbial communities and their assembly processes. Reductions of bacterial-fungal microbial network structural integrity and stability were associated with skin conditions (acne and dandruff). Multivariate analysis revealed associations between abundances of Propionibacterium and Malassezia with host properties and pollutant exposure levels. Shannon diversity increase was correlated to exposure levels of PAHs in a dose-dependent manner. Shotgun metagenomics analysis of samples (n = 32) from individuals of the lowest and highest exposure levels of PAHs further highlighted associations between the PAHs quantified and decrease in abundances of skin commensals and increase in oral bacteria. Functional analysis identified associations between levels of PAHs and abundance of microbial genes of metabolic and other pathways with potential importance in host-microbe interactions as well as degradation of aromatic compounds. Conclusions: The results in this study demonstrated the changes in composition and functional capacities of the cutaneous microbiota associated with chronic exposure levels of PAHs. Findings from this study will aid the development of strategies to harness the microbiota in protecting the skin against pollutants.

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Section: Analysis Of Pahs In Hair Samplesmentioning

confidence: 99%

Changes of the human skin microbiota upon chronic exposure to polycyclic aromatic hydrocarbon pollutants

Leung¹,

Tong²,

Bastien³

et al. 2020

Microbiome

View full text Add to dashboard Cite

show abstract

“…Early embryonic development may be a particularly susceptible period for PAH exposure, resulting in increased PAH-DNA adducts and altered DNA methylation. Grova et al (2017) identified new tetrahydroxylated polycyclic aromatic hydrocarbons (tetra-OH-PAHs) released from the hydrolysis of DNA-adducts in hair, which might be useful for the biomonitoring of human internal exposure to PAHs. The relevance of tetra-OH-PAH analysis in hair as a biomarker of PAH exposure was evaluated in a dose-response study in which 64 rats were repeatedly exposed (three times per week) to a mixture of 16 PAHs at low doses (0.01 to 0.8 mg/kg) for 90 days.…”

Section: Biomarkers Of Pah Exposurementioning

confidence: 99%

Analytical chemistry, formation, mitigation, and risk assessment of polycyclic aromatic hydrocarbons: From food processing to in vivo metabolic transformation

Zhang

Chen

Zhang

2021

Comp Rev Food Sci Food Safe

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Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in the air, oils, water, and food products we encounter every day. Among these exposures, food consumption is a major route of PAH exposure for nonsmokers. The PAH dietary exposure levels vary among different countries; however, many studies have shown PAH exposure to be highly concerning to human health. The levels of PAH contamination in food are mainly influenced by processing procedures and cooking methods, and they could be attenuated by modifying cooking procedures and adding antioxidant‐rich marinades. Several PAHs have toxic, mutagenic, and carcinogenic properties. The PAH benzo[a]pyrene (BaP) is particularly regarded as carcinogenic. There are three major metabolism pathways for PAHs, and the final products can bind to DNA, thus exerting mutagenic effects. Biological monitoring through the use of biomarkers is necessary for comprehensive and accurate risk assessments of human PAH exposure. It is important to reduce dietary PAH exposure and to implement reasonable and effective risk management strategies to reduce PAH levels in food to improve public health.

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Role of Pet Dogs and Cats as Sentinels of Human Exposure to Polycyclic Aromatic Hydrocarbons

Rial-Berriel

Henríquez-Hernández

Luzardo

2019

Pets as Sentinels, Forecasters and Promoters of Human Health

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Identification of new tetrahydroxylated metabolites of Polycyclic Aromatic Hydrocarbons in hair as biomarkers of exposure and signature of DNA adduct levels

Cited by 15 publications

References 29 publications

Changes of the human skin microbiota upon chronic exposure to polycyclic aromatic hydrocarbon pollutants

Changes of the human skin microbiota upon chronic exposure to polycyclic aromatic hydrocarbon pollutants

Analytical chemistry, formation, mitigation, and risk assessment of polycyclic aromatic hydrocarbons: From food processing to in vivo metabolic transformation

Role of Pet Dogs and Cats as Sentinels of Human Exposure to Polycyclic Aromatic Hydrocarbons

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