Identification of Nodal-dependent enhancer of amphioxus Chordin sufficient to drive gene expression into the chordate dorsal organizer

Machacova, Simona; Kozmík, Zbyněk; Kozmiková, Iryna

doi:10.1007/s00427-022-00698-z

Cited by 3 publications

(3 citation statements)

References 33 publications

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“…This reporter also exhibited strong activity in the axial mesoderm, similar to its endogenous pattern. Likewise, BfChordin::EGFP ( 18 ) drove expression in the axial domain and PrCP at the 80% epiboly stage in zebrafish (Fig. 5 B A-A’ ), more closely aligning with the endogenous expression of Chordin in amphioxus than with that in zebrafish ( 58 ).…”

Section: Resultsmentioning

confidence: 74%

“…The embryos of F1 and later generations were analysed with in-situ hybridization. Previously generated transgenic line containing BfCordin::EGFP ( 18 ) was analysed with light sheet fluorescent microscopy. The embryos were imaged with a Zeiss Light Sheet Z.1 microscope and processed with Imarisx64 9.6.0 and Fiji ImageJ software.…”

Section: Methodsmentioning

confidence: 99%

“…Several studies demonstrated the existence of a gastrula organizer in amphioxus, with a compelling degree of homology to the dorsal organizer of vertebrates (15)(16)(17)(18). Despite this, a prevailing view suggests the absence of a PrCP in amphioxus (4,19).…”

Section: Main Textmentioning

confidence: 99%

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Cell type and regulatory analysis in amphioxus illuminates evolutionary origin of the vertebrate head

Markos,

Kubovciak,

Mrstakova

et al. 2024

Preprint

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To shed light on the enigmatic origin of the vertebrate head, our study employs an integrated approach that combines single-cell transcriptomics, perturbations in signalling pathways, and cis-regulatory analysis in amphioxus, a close relative of chordate common ancestor. Through cell type characterization, we identified the presence of a prechordal plate, pre-migratory and migratory neural crest-like cell populations in the developing amphioxus embryo. Functional analysis established conserved roles of the Nodal and Hedgehog signalling pathways in prechordal plate, and of Wnt signalling pathway in neural crest development. Furthermore, the trans-species transgenic experiments provided evidence of cis-regulatory level homology within the chordate lineage. Our findings provide evidence that the key features of vertebrate head development can be traced back to the common ancestor of all chordates.One Sentence SummaryCell populations forming the vertebrate head are present in the close relative of chordate common ancestor.

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Section: Resultsmentioning

confidence: 74%

Section: Methodsmentioning

confidence: 99%

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Cell type and regulatory analysis in amphioxus illuminates evolutionary origin of the vertebrate head

Markos,

Kubovciak,

Mrstakova

et al. 2024

Preprint

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From sequence to consequence: Deciphering the complex cis-regulatory landscape

Dsilva,

Galande

2024

J Biosci

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Cell type and regulatory analysis in amphioxus illuminates evolutionary origin of the vertebrate head

Markos,

Kubovciak,

Mikula Mrstakova

et al. 2024

Nat Commun

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To shed light on the enigmatic origin of the vertebrate head, our study employs an integrated approach that combines single-cell transcriptomics, perturbations in signaling pathways, and cis-regulatory analysis in amphioxus. As a representative of a basal lineage within the chordate phylum, amphioxus retains many characteristics thought to have been present in the common chordate ancestor. Through cell type characterization, we identify the presence of prechordal plate-like, pre-migratory, and migratory neural crest-like cell populations in the developing amphioxus embryo. Functional analysis establishes conserved roles of the Nodal and Hedgehog signaling pathways in prechordal plate-like populations, and of the Wnt signaling pathway in neural crest-like populations’ development. Furthermore, our trans-species transgenic experiments highlight similarities in the regulatory environments that drive neural crest-like and prechordal plate-like developmental programs in both vertebrates and amphioxus. Our findings provide evidence that the key features of vertebrate head development can be traced back to the common ancestor of all chordates.

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Identification of Nodal-dependent enhancer of amphioxus Chordin sufficient to drive gene expression into the chordate dorsal organizer

Cited by 3 publications

References 33 publications

Cell type and regulatory analysis in amphioxus illuminates evolutionary origin of the vertebrate head

Cell type and regulatory analysis in amphioxus illuminates evolutionary origin of the vertebrate head

From sequence to consequence: Deciphering the complex cis-regulatory landscape

Cell type and regulatory analysis in amphioxus illuminates evolutionary origin of the vertebrate head

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