Identification of non-tuberculosis mycobacteria isolated from cattle in the Republic of Tatarstan

Kamalieva, Y.R.; Mingaleev, D.N.; Равилов, Р Х

doi:10.32634/0869-8155-2021-354-11-12-32-35

Cited by 2 publications

(1 citation statement)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…По мере снижения распространения туберкулеза крупного рогатого скота и усиления мер по диагностике для выявления остаточной инфекции на территориях, где были внедрены программы борьбы с этой патологией, обнаруживалось увеличение числа микобактериозов, обусловленных нетуберкулезными микобактериями [7,8,9,10]. Несмотря на растущий интерес к этой проблеме, опубликованных данных о нетуберкулезных микобактериальных инфекциях по-прежнему мало, а имеющаяся литература в основном сосредоточена на комплексе Mycobacterium avium и его подвидах [11,12,13,14,15].…”

Section: Introductionunclassified

Studying immunotherapeutic properties of the conjugate based on BCG antigens with betulonic acid in guinea pigs infected with <i>Mycobacterium scrofulaceum</i>

Koshkin,

Vlasenko,

Dengis

2024

Veterinariâ segodnâ

View full text Add to dashboard Cite

The paper reports on the research into the immunotherapeutic properties of a conjugate based on BCG antigens with betulonic acid after experimental infection of guinea pigs with Mycobacterium scrofulaceum culture, belonging to nontuberculosis mycobacteria type II according to the Runyon classification. Fifteen guinea pigs were used for the experimental purposes, divided into 3 groups. Mycobacterium scrofulaceum was subcutaneously injected into animals of Groups 1 and 2 (n = 10) at a dose of 5 mg. Fourteen days later, a conjugate based on BCG antigens with betulonic acid was subcutaneously injected into animals of Group 2 (n = 5) at a dose of 500 µg/mL of protein. Five intact animals were used as controls. During the experiment, neutrophil bactericidal activity was assessed, and histopathological examination of inguinal lymph nodes was done. The experiment showed that the inoculation of Mycobacterium scrofulaceum into guinea pigs activates cationic proteins and neutrophil myeloperoxidase, and on experiment day 42 (preceded by mycobacteria withdrawal from the body) their concentration reduced to the level of the control group. The vaccine administration induced a more active intracellular phagocyte metabolism during the entire observation period, which resulted in the elimination of nontuberculosis mycobacteria in animals as early as day 7 after treatment with the conjugate. The elimination was confirmed by the absence of mycobacterial antigen in blood smears tested in indirect immunofluorescence, as well as by histopathological changes in inguinal lymph nodes demonstrated as a reduction of germinal centers within lymphoid follicles.

show abstract

Section: Introductionunclassified

Studying immunotherapeutic properties of the conjugate based on BCG antigens with betulonic acid in guinea pigs infected with <i>Mycobacterium scrofulaceum</i>

Koshkin,

Vlasenko,

Dengis

2024

Veterinariâ segodnâ

View full text Add to dashboard Cite

show abstract

Change in hepatocyte nuclear-cytoplasmic ratio at nontuberculosis mycobacteria infection against the background of immunomodulator action

Kosobokov,

Dudoladova

2024

Veterinariâ segodnâ

View full text Add to dashboard Cite

The study was targeted at the examination of the effect of the specific immunomodulator KIM-M2 on the morphostructure of liver cells of guinea pigs infected with nontuberculosis mycobacteria. The research was carried out in 15 guinea pigs selected by gender at the Diagnostic Research and Biotechnology Laboratory of the Department of Veterinary Medicine of the Omsk Agrarian Scientific Center. All animals were kept in a specialized animal keeping facilities according to standard housing and feeding regime. The experimental animals were subdivided into three groups of five animals in each: group 1 – challenge group (Mycobacterium scrofulaceum), group 2 – experimental group (Mycobacterium scrofulaceum and KIM-M2), group 3 – pure control group (saline solution). On day 30 after the start of the experiment, the animals were removed from the experiment, liver biopsy samples were collected and histologic specimens were prepared according to the classical method. During the experiment, it was found that KIM-M2 had a regenerative effect on the liver tissue of the guinea pigs infected with nontuberculosis mycobacteria, which was associated with 1.5-fold increase in the number of mononuclear hepatocytes, 3-fold increase in binuclear cells and 4.3-fold decrease in anucleate hepatocytes thus indicating the manifestation of compensatory reactions in the organ and increase in the depth of regenerative processes. As for animals in group 1; 1.8and 1.3-fold increase in the area of the nucleus and cytoplasm as compared with the individuals in group 2, and 2.7and 2-fold increase as compared with the animals in the control group, respectively, indicated the launch of the accumulation mechanisms of the potential reparative reserves and increase in their depth in the liver tissues.

show abstract

Identification of non-tuberculosis mycobacteria isolated from cattle in the Republic of Tatarstan

Cited by 2 publications

References 5 publications

Studying immunotherapeutic properties of the conjugate based on BCG antigens with betulonic acid in guinea pigs infected with <i>Mycobacterium scrofulaceum</i>

Studying immunotherapeutic properties of the conjugate based on BCG antigens with betulonic acid in guinea pigs infected with <i>Mycobacterium scrofulaceum</i>

Change in hepatocyte nuclear-cytoplasmic ratio at nontuberculosis mycobacteria infection against the background of immunomodulator action

Contact Info

Product

Resources

About