The purpose of this work was to identify mycobacteria of non-tuberculosis type isolated from environmental objects in the Republic of Tatarstan. The article presents the result of identification of mycobacteria of non-tuberculous type in samples of washout from environmental objects obtained from farms with an identified species list of circulating mycobacteria of non-tuberculous type from reacting to tuberculin cattle by polymerase chain reaction in real time. In the course of our research, we found that nonspecific allergic reactions to bovine tuberculin in these farms are caused by contamination with mycobacteria of a non-tuberculosis type of livestock premises and soils near farms.

The purpose of this work was to identify non-tuberculosism ycobacteria isolated from cattle in the Republic of Tatarstan. The article presents the results of identification of non-tuberculosis mycobacteria in samples of pathological material received from cattle reacting to tuberculin by polymerase chain reaction in real time and with electrophoretic detection. In the result of our research it is determined that in 43% of the explored samples of pathological material received from reacting to tuberculin cattle nontuberculous mycobacteria were identified.

The purpose of this work was to develop highly specific oligonucleotide primers and probes for the identification of non-tuberculosis mycobacteria in pathological material taken from tuberculin-responsive cattle. The article presents the result of the development and design of a set of oligonucleotide primers and probes, research of their specificity and sensitivity. The primers and probes developed by us for the identification of non-tuberculosis mycobacteria in order to differentiate nonspecific reactions to tuberculin in cattle have high specificity and sensitivity.