Identification of Nontuberculous Mycobacteria from Clinical Isolates and Specimens using AdvanSure Mycobacteria GenoBlot Assay

Abstract

The aim of this study was to evaluate the clinical performance of AdvanSure GenoBlot assay using NTM isolates and clinical specimens. A total of 136 NTM isolates and 176 clinical specimens were included in this study. AdvanSure Mycobacteria GenoBlot assay was performed according to the manufacturer's instructions. We compared the results with those of 16S rRNA and rpoB genes sequencing. Of the total 136 isolates, 111 (81.6%) were correctly identified to the species level by the GenoBlot assay. The final concordance rate was 89.7% (122/136) when including 11 GPC results for uncommon NTM. The most common NTM, M. avium, M. fortuitum, M. gordonae, M. intracellulare, M. chelonae, M. abscessus, and M. kansasii, were correctly identified by the GenoBlot assay. For 176 organisms in clinical specimens, 117 were identified to the species level, including a single species for 111 and two species for 6. The final detection and identification rate for clinical specimens was 94.9% and 66.5%, respectively. The AdvanSure GenoBlot assay performs well in identifying most common NTM and would be useful in a clinical laboratory.

Whole-Genome Sequencing Analysis to Identify Infection with Multiple Species of Nontuberculous Mycobacteria

“…In addition, there is no gold standard for detection and identification of multiple species infection of NTM. LPA and multiplex real-time PCR/melting curve analysis have been applied to detect mixed infections with different NTM species based on the 16S-23S rRNA intergenic transcribed spacer (ITS) region and achieved good performance using material directly from clinical samples [ 16 , 17 , 18 ]. However, the resolution of these techniques is still low because not all NTM species, especially newly discovered species, have not been included in assay development.…”

Identification and characterization of nontuberculous mycobacteria isolated from suspected pulmonary tuberculosis patients in eastern china from 2009 to 2019 using an identification array system

“…Compared with other assays, the CapitalBio Mycobacterium identification array system shows higher sensitivity and specificity. 36 It has been reported that the sensitivity and specificity of the CapitalBio Mycobacterium identification microarray system were 99.6% and 100%, respectively, compared with the results of 16S rRNA gene sequencing when used with clinical isolates from respiratory specimens. 16 Interestingly, it can identify up to 17 Mycobacterium species.…”

Isolation and Antimicrobial Susceptibility of Nontuberculous Mycobacteria in a Tertiary Hospital in Korea, 2016 to 2020

“…NTM species identification was performed using the AdvanSure Mycobacteria GenoBlot assay (LG Chem) at KIT. The assay can identify Mycobacterium tuberculosis complex and 20 different NTM species 24 . The reverse line probe assay can detect the presence of ≥2 NTM species infection in a single specimen.…”