Identification of novel heat shock factor‐dependent genes and biochemical pathways in <i>Arabidopsis thaliana</i>

Busch, Wolfgang; Wunderlich, Markus; Schöffl, Fritz

doi:10.1111/j.1365-313x.2004.02272.x

Cited by 344 publications

(396 citation statements)

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“…Although these two herbicides interfere with the synthesis of branched-chain amino acids, an herbicide that targets photosystem II, bromoxynil, did not induce PEN3 expression (Glombitza et al, 2004). Abscisic acid and heat shock treatments downregulated the expression of PEN3 (see Supplemental Table 2 online) (Busch et al, 2005). Thus, PEN3 expression is altered by pathogens and a subset of abiotic stresses.…”

Section: Pen3 Encodes a Pdr-like Abc Transportermentioning

confidence: 99%

ArabidopsisPEN3/PDR8, an ATP Binding Cassette Transporter, Contributes to Nonhost Resistance to Inappropriate Pathogens That Enter by Direct Penetration

et al. 2006

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Arabidopsis thaliana is a host to the powdery mildew Erysiphe cichoracearum and nonhost to Blumeria graminis f. sp hordei, the powdery mildew pathogenic on barley (Hordeum vulgare). Screening for Arabidopsis mutants deficient in resistance to barley powdery mildew identified PENETRATION3 (PEN3). pen3 plants permitted both increased invasion into epidermal cells and initiation of hyphae by B. g. hordei, suggesting that PEN3 contributes to defenses at the cell wall and intracellularly. pen3 mutants were compromised in resistance to the necrotroph Plectosphaerella cucumerina and to two additional inappropriate biotrophs, pea powdery mildew (Erysiphe pisi) and potato late blight (Phytophthora infestans). Unexpectedly, pen3 mutants were resistant to E. cichoracearum. This resistance was salicylic acid–dependent and correlated with chlorotic patches. Consistent with this observation, salicylic acid pathway genes were hyperinduced in pen3 relative to the wild type. The phenotypes conferred by pen3 result from the loss of function of PLEIOTROPIC DRUG RESISTANCE8 (PDR8), a highly expressed putative ATP binding cassette transporter. PEN3/PDR8 tagged with green fluorescent protein localized to the plasma membrane in uninfected cells. In infected leaves, the protein concentrated at infection sites. PEN3/PDR8 may be involved in exporting toxic materials to attempted invasion sites, and intracellular accumulation of these toxins in pen3 may secondarily activate the salicylic acid pathway.

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Section: Pen3 Encodes a Pdr-like Abc Transportermentioning

confidence: 99%

ArabidopsisPEN3/PDR8, an ATP Binding Cassette Transporter, Contributes to Nonhost Resistance to Inappropriate Pathogens That Enter by Direct Penetration

et al. 2006

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“…The expression of most HsfA2-regulated genes appears also to be dependent on HsfA1a/HsfA1b (Busch et al, 2005). Although HsfA1a/HsfA1b are not induced by either heat or anoxia (Fig.…”

Section: Hsfa2 Overexpression Confers Tolerance To Anoxiamentioning

confidence: 99%

“…Interestingly, it seems that in Arabidopsis, different from tomato (Solanum lycopersicum; Mishra et al, 2002), there is not a unique master regulator for the Hsf-mediated heat shock response. Different Hsfs have been identified as potential crucial element for Arabidopsis response not only to heat stress (Busch et al, 2005;Schramm et al, 2006Schramm et al, , 2008Charng et al, 2007) but also to oxidative stress (Li et al, 2005;Nishizawa et al, 2008), hydrogen peroxide (H 2 O 2 ), high light (Nishizawa et al, 2006), and salt stress as well (Ogawa et al, 2007).…”

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The Heat-Inducible Transcription Factor HsfA2 Enhances Anoxia Tolerance in Arabidopsis

et al. 2010

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Anoxia induces several heat shock proteins, and a mild heat pretreatment can acclimatize Arabidopsis (Arabidopsis thaliana) seedlings to subsequent anoxic treatment. In this study, we analyzed the response of Arabidopsis seedlings to anoxia, heat, and combined heat + anoxia stress. A significant overlap between the anoxic and the heat responses was observed by wholegenome microarray analysis. Among the transcription factors induced by both heat and anoxia, the heat shock factor A2 (HsfA2), known to be involved in Arabidopsis acclimation to heat and to other abiotic stresses, was strongly induced by anoxia. Heat-dependent acclimation to anoxia is lost in an HsfA2 knockout mutant (hsfa2) as well as in a double mutant for the constitutively expressed HsfA1a/HsfA1b (hsfA1a/1b), indicating that these three heat shock factors cooperate to confer anoxia tolerance. Arabidopsis seedlings that overexpress HsfA2 showed an increased expression of several known targets of this transcription factor and were markedly more tolerant to anoxia as well as to submergence. Anoxia failed to induce HsfA2 target proteins in wild-type seedlings, while overexpression of HsfA2 resulted in the production of HsfA2 targets under anoxia, correlating well with the low anoxia tolerance experiments. These results indicate that there is a considerable overlap between the molecular mechanisms of heat and anoxia tolerance and that HsfA2 is a player in these mechanisms.

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“…It has been reported that the expression of Arabidopsis GolS1 and GolS2 is regulated by a heat shock transcription factor (HSF), such as HsfA1a, HsfA1b, and/or HsfA2 (Panikulangara et al, 2004;Busch et al, 2005;Schramm et al, 2006). Recently, we reported that the transcription of GolS1 and GolS2 is induced by a combination of high-light and heat stress or treatment with hydrogen peroxide, as is the transcription of HsfA2 (Nishizawa et al, 2006).…”

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Galactinol and Raffinose Constitute a Novel Function to Protect Plants from Oxidative Damage

2008

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Galactinol synthase (GolS) is a key enzyme in the synthesis of raffinose family oligosaccharides that function as osmoprotectants in plant cells. In leaves of Arabidopsis (Arabidopsis thaliana) plants overexpressing heat shock transcription factor A2 (HsfA2), the transcription of GolS1, -2, and -4 and raffinose synthase 2 (RS2) was highly induced; thus, levels of galactinol and raffinose increased compared with those in wild-type plants under control growth conditions. In leaves of the wild-type plants, treatment with 50 mM methylviologen (MV) increased the transcript levels of not only HsfA2, but also GolS1, -2, -3, -4, and -8 and RS2, -4, -5, and -6, the total activities of GolS isoenzymes, and the levels of galactinol and raffinose. GolS1-or GolS2-overexpressing Arabidopsis plants (Ox-GolS1-11, Ox-GolS2-8, and Ox-GolS2-29) had increased levels of galactinol and raffinose in the leaves compared with wild-type plants under control growth conditions. High intracellular levels of galactinol and raffinose in the transgenic plants were correlated with increased tolerance to MV treatment and salinity or chilling stress. Galactinol and raffinose effectively protected salicylate from attack by hydroxyl radicals in vitro. These findings suggest the possibility that galactinol and raffinose scavenge hydroxyl radicals as a novel function to protect plant cells from oxidative damage caused by MV treatment, salinity, or chilling.

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Identification of novel heat shock factor‐dependent genes and biochemical pathways in Arabidopsis thaliana

Cited by 344 publications

References 50 publications

ArabidopsisPEN3/PDR8, an ATP Binding Cassette Transporter, Contributes to Nonhost Resistance to Inappropriate Pathogens That Enter by Direct Penetration

ArabidopsisPEN3/PDR8, an ATP Binding Cassette Transporter, Contributes to Nonhost Resistance to Inappropriate Pathogens That Enter by Direct Penetration

The Heat-Inducible Transcription Factor HsfA2 Enhances Anoxia Tolerance in Arabidopsis

Galactinol and Raffinose Constitute a Novel Function to Protect Plants from Oxidative Damage

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