Identification of Novel Loci and Candidate Genes for Resistance to Powdery Mildew in a Resequenced Cucumber Germplasm

Li, Xiaoping; Gu, Xingfang; Lu, Hongwei; Liu, Panna; Han, Moonsik; Bai, Yuling; Zhang, Shengping

doi:10.3390/genes12040584

Cited by 21 publications

(15 citation statements)

References 34 publications

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“…SNP Lalb_Chr05_3706534 is located in the non-coding region of Lalb_Chr05g0217341. Homologs of Lalb_Chr05g0217341 in closely related legume species encode for paired amphipathic helix protein Sin3-like 4 proteins, which are known to be involved in powdery mildew ( Podosphaera fusca ) resistance in cucumber (Liu et al 2021 ). SNP Lalb_Chr05_3784474 is considered linked to Lalb_Chr05_3706534 and is located within the gene Lalb_Chr05g0217471 which encodes a LRR containing receptor-like protein with a serine/threonine kinase.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide association study reveals white lupin candidate gene involved in anthracnose resistance

Alkemade

N.²,

Messmer

et al. 2022

Theor Appl Genet

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Key message GWAS identifies candidate gene controlling resistance to anthracnose disease in white lupin. Abstract White lupin (Lupinus albus L.) is a promising grain legume to meet the growing demand for plant-based protein. Its cultivation, however, is severely threatened by anthracnose disease caused by the fungal pathogen Colletotrichum lupini. To dissect the genetic architecture for anthracnose resistance, genotyping by sequencing was performed on white lupin accessions collected from the center of domestication and traditional cultivation regions. GBS resulted in 4611 high-quality single-nucleotide polymorphisms (SNPs) for 181 accessions, which were combined with resistance data observed under controlled conditions to perform a genome-wide association study (GWAS). Obtained disease phenotypes were shown to highly correlate with overall three-year disease assessments under Swiss field conditions (r > 0.8). GWAS results identified two significant SNPs associated with anthracnose resistance on gene Lalb_Chr05_g0216161 encoding a RING zinc-finger E3 ubiquitin ligase which is potentially involved in plant immunity. Population analysis showed a remarkably fast linkage disequilibrium decay, weak population structure and grouping of commercial varieties with landraces, corresponding to the slow domestication history and scarcity of modern breeding efforts in white lupin. Together with 15 highly resistant accessions identified in the resistance assay, our findings show promise for further crop improvement. This study provides the basis for marker-assisted selection, genomic prediction and studies aimed at understanding anthracnose resistance mechanisms in white lupin and contributes to improving breeding programs worldwide.

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Section: Discussionmentioning

confidence: 99%

Genome-wide association study reveals white lupin candidate gene involved in anthracnose resistance

Alkemade

N.²,

Messmer

et al. 2022

Theor Appl Genet

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“…The single recessive gene Csa5M622830 , which encodes a GATA transcriptional factor, is likely the gene for the complete PM resistance introgressed from Cucumis hystrix ( Zhang et al, 2018 ). CsGy5G015660 , which encodes a putative leucine-rich repeat receptor-like serine/threonine-protein kinase, is currently considered a strong candidate gene for PM resistance in cucumber ( Liu et al, 2021 ; Zhang C. et al, 2021 ). Moreover, proteins related to PM resistance have also been identified and functionally verified.…”

Section: Inheritance Quantitative Trait Loci Mapping and Candidate Ge...mentioning

confidence: 99%

“… Call et al (2012b) identified three highly DM-resistant materials, PI 197088, PI 330628, and PI 605996, from 1,300 cucumber collections. Among them, PI 197088 is the most studied for DM resistance, with multiple genes being controlled in breeding programs ( Li et al, 2018 ; Liu et al, 2021 ). PI 197088 also has high resistance to PM.…”

Section: Inheritance Quantitative Trait Loci Mapping and Candidate Ge...mentioning

confidence: 99%

“…In total, five QTLs ( dm2.2 , dm4.1 , dm5.1 , dm5.2 , and dm6.1 ) have been identified and dm2.2 has the largest effect on DM resistance as assessed by combining BSA and NGS methods based on SNP markers ( Win et al, 2017 ). Additionally, 18 QTLs have been detected through the GWAS of a core database of 97 cucumber lines, but only six QTLs ( dmG1.4 , dmG4.1 , dmG4.3 , dmG5.2 , dmG7.1 , and dmG7.2 ) are associated with stable DM resistance ( Liu et al, 2021 ). To date, PI 197085, PI 197088, WI 7120 (PI 330628), WI 2757, S94, TH118FLM, IL52, and K8 have been used for mapping QTLs associated with DM resistance.…”

Section: Inheritance Quantitative Trait Loci Mapping and Candidate Ge...mentioning

confidence: 99%

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Research Advances in Genetic Mechanisms of Major Cucumber Diseases Resistance

Wei

Yan

et al. 2022

Front. Plant Sci.

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Cucumber (Cucumis sativus L.) is an important economic vegetable crop worldwide that is susceptible to various common pathogens, including powdery mildew (PM), downy mildew (DM), and Fusarium wilt (FM). In cucumber breeding programs, identifying disease resistance and related molecular markers is generally a top priority. PM, DM, and FW are the major diseases of cucumber in China that cause severe yield losses and the genetic-based cucumber resistance against these diseases has been developed over the last decade. Still, the molecular mechanisms of cucumber disease resistance remain unclear. In this review, we summarize recent findings on the inheritance, molecular markers, and quantitative trait locus mapping of cucumber PM, DM, and FM resistance. In addition, several candidate genes, such as PM, DM, and FM resistance genes, with or without functional verification are reviewed. The data help to reveal the molecular mechanisms of cucumber disease resistance and provide exciting new opportunities for further resistance breeding.

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“…Cucumber plants have multiple mechanisms to resist fungal infections, and the interactions between cucumbers and PM fungi are strongly influenced by resistance genes. While previous studies have confirmed that cucumber PM resistance is controlled by multiple resistance loci, most of these studies have been limited to quantitative trait locus mapping ( Shen, 2009 ; Yu, 2015 ; Wang et al, 2020 ; Liu et al, 2021 ). At present, only a few recessive resistance genes, mainly including Mildew Resistance Locus O ( MLO ) and NBS-LRR -type resistance gene families, have been successfully cloned and validated ( Nie et al, 2015 ; Shi, 2016 ; Wang et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Sequence Analysis of the Defense Responses of Resistant and Susceptible Cucumber Strains to Podosphaera xanthii

Meng

Song

et al. 2022

Front. Plant Sci.

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Powdery mildew (PM) caused by Podosphaera xanthii poses a continuous threat to the performance and yield of the cucumber (Cucumis sativus L.). Control in the initial stages of infection is particularly important. Here, we studied the differential physiological and transcriptomic changes between PM-resistant strain B21-a-2-1-2 and PM-susceptible strain B21-a-2-2-2 at the early stage of P. xanthii attack. When challenged with P. xanthii, the tolerant line can postpone the formation of the pathogen primary germ. Comparative transcriptomic analysis suggested that DEGs related to the cell wall and to pathogen and hormone responses were similar enriched in both cucumber lines under P. xanthii infection. Notably, the number of DEGs triggered by P. xanthii in B21-a-2-1-2 was quintuple that in B21-a-2-2-2, revealing that the success of defense of resistant cucumber is due to rapidly mobilizing multiple responses. The unique responses detected were genes related to SA signaling, MAPK signaling, and Dof and WRKY transcription factors. Furthermore, 5 P. xanthii -inducible hub genes were identified, including GLPK, ILK1, EIN2, BCDHβ1, and RGGA, which are considered to be key candidate genes for disease control. This study combined multiple analytical approaches to capture potential molecular players and will provide key resources for developing cucumber cultivars resistant to pathogen stress.

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Identification of Novel Loci and Candidate Genes for Resistance to Powdery Mildew in a Resequenced Cucumber Germplasm

Cited by 21 publications

References 34 publications

Genome-wide association study reveals white lupin candidate gene involved in anthracnose resistance

Genome-wide association study reveals white lupin candidate gene involved in anthracnose resistance

Research Advances in Genetic Mechanisms of Major Cucumber Diseases Resistance

Transcriptome Sequence Analysis of the Defense Responses of Resistant and Susceptible Cucumber Strains to Podosphaera xanthii

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