Identification of novel mammalian hosts and Brazilian biome geographic distribution of Trypanosoma cruzi TcIII and TcIV

Barros, Juliana H. S.; Xavier, Samanta Cristina das Chagas; Bilac, Daniele; Lima, Valdirene S.; Dario, Maria Augusta; Jansen, Ana Maria

doi:10.1016/j.actatropica.2017.05.003

Cited by 24 publications

(26 citation statements)

References 49 publications

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“…Armadillos, especially D. novemcinctus, have been reported as natural hosts of TcIII throughout South America [11,40,41], thus the absence of TcII/V/VI infections from all 49 armadillos was not surprising. Although Chagas Sero K-SeT was shown to be able to detect infection in armadillo [20], positive test line intensity was weak; Protein A may be more appropriate than Protein G for binding of armadillo IgG, as others have reported [42], and which we observed here.…”

Section: Discussionmentioning

confidence: 99%

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A lineage-specific rapid diagnostic test (Chagas Sero K-SeT) identifies Brazilian Trypanosoma cruzi II/V/VI reservoir hosts among diverse mammalian orders

et al. 2020

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Trypanosoma cruzi, the protozoan agent of Chagas disease in the Americas, is comprised of six genetic lineages (TcI-TcVI) and a possible seventh (TcBat, related to TcI). Identification of T. cruzi lineages infecting reservoir mammalian species is fundamental to resolving transmission cycles. However, this is hindered by the limited sensitivity and technical complexity of parasite isolation and genotyping. An alternative approach is serology using T. cruzi lineage-specific epitopes, such as those of the trypomastigote small surface antigen (TSSA). For surveillance of T. cruzi lineage infections in mammal species from diverse Brazilian regions, we apply a novel rapid diagnostic test (RDT, Chagas Sero K-SeT), which incorporates the TSSA peptide epitope specific to TcII/V/VI (TSSApep-II/V/VI) and Protein G detection of antibodies. Chagas Sero K-SeT RDT results with sera from experimentally infected mice, from tamarin primates (Leontopithecus spp.) and from canines (Canis familiaris) were concordant with corresponding TSSApep-II/V/VI ELISAs. The Chagas Sero K-Set detected TcII/V/VI infections in Leontopithecus spp. from the Atlantic forest (n = 46), in C. familiaris (n = 16) and Thrichomys laurentius (n = 2) from Caatinga biome and Chiroptera (n = 1) from Acre, Amazonia. The Chagas Sero K-SeT RDT is directly applicable to TcII/V/ VI-specific serological surveillance of T. cruzi infection in several different mammalian Orders. It can replace ELISAs and provides efficient, point-of-sampling, low-cost detection of TcII/V/VI infections, with at least equivalent sensitivity, although some mammals may be difficult to trap, and, not unexpectedly, Chagas Sero K-SeT could not recognise feline IgG. Knowledge of sylvatic hosts of T. cruzi can be expanded, new reservoir species discovered, and the ecology of transmission cycles clarified, particularly with adaptation to further mammalian Orders.

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Section: Discussionmentioning

confidence: 99%

“…No armadillo-specific conjugate was available for ELISA; this also encourages further deployment of Protein A for these important reservoirs hosts. Opossums have been identified as common hosts of TcI but rarely reported as hosts of TcII [11,43].…”

Section: Discussionmentioning

confidence: 99%

A lineage-specific rapid diagnostic test (Chagas Sero K-SeT) identifies Brazilian Trypanosoma cruzi II/V/VI reservoir hosts among diverse mammalian orders

et al. 2020

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“…We observed Trypanosoma cruzi infection in Didelphis albiventris, Oecomys cleberi, Rhipidomys macrurus, and G. agilis, with the latter being the only species that displayed patent parasitemia and in which a distinguished diversity of DTUs was detected. We found other trypanosomatid species (T. rangeli, T. dionisii, Leishmania braziliensis, and L. guyanensis) in D. albiventris, O. cleberi, C. expulsus, and G. agilis showing a higher diversity of trypanosomatids in small mammals (n = 9) than that observed in wild canids (n = 2) of the same area [23,24].…”

Section: Discussionmentioning

confidence: 63%

“…DTU TcII is also a widely distributed genotype and it has been found to infect several mammalian taxa in distinct habitats and biomes [35][36][37]. Although DTUs TcIII and TcIV are widely distributed and may infect a wide variety of mammals, they appear to occur at significantly lower rates, most likely because they are maintained in very low parasitemia in nature, which hampers their detection [23,32]. Although classically associated to armadillos [38,39], T. cruzi TcIII has also proved to be able to infect wild rodents, marsupials, carnivores, and artiodactyls, as well domestic dogs [5,23].…”

Section: Discussionmentioning

confidence: 99%

“…As a result of this work, infection by T. cruzi and Leishmania spp. in these wild canids has been diagnosed through serological assays, besides the isolation of T. cruzi DTU TcIII in hoary foxes [22,23]. Aiming to investigate the diversity of trypanosomatid species in the area (and possible infection overlaps among hosts from distinct taxa, as small mammals and canids), we surveyed the infection by Trypanosoma spp.…”

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Trypanosomatids in Small Mammals of an Agroecosystem in Central Brazil: Another Piece in the Puzzle of Parasite Transmission in an Anthropogenic Landscape

et al. 2019

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We surveyed infection by Trypanosoma spp. and Leishmania spp. in small wild mammals from Cumari, Goiás State aiming to investigate the diversity of trypanosomatid in a modified landscape of the Brazilian Cerrado (and possible infection overlapping with canids from the same area). Blood, skin, spleen, and liver samples were collected for parasitological, serological, and molecular assays. Gracilinanus agilis was the most abundant species (N = 70; 48.6%) and it was the only one with patent parasitemia. Characterization by mini-exon and 18SrDNA targets were achieved in 7/10 hemocultures with positive fresh blood examination, which confirmed the T. cruzi infection by Discrete Typing Units (DTU) TcI in single (N = 2) and mixed infections with other DTUs (N = 5). T. rangeli and T. dionisii were detected in skin fragments from Didelphis albiventris and Oecomys cleberi, respectively. G. agilis were found to be infected by L. braziliensis and L. guyanensis, while Leishmania sp. DNA was detected in the liver of Oligoryzomys nigripes and Calomys expulsus. Subpatent infection by T. cruzi and Leishmania sp. was serologically detected in 15% and 9% of the small mammal fauna, respectively. Small mammals from Cumari are included in T. cruzi and Leshmania spp. transmission cycles, showing a higher diversity of trypanosomatid species and/or genotypes than that observed in canids of the same agroecosystem.

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The influence of abiotic and biotic variables on the patent parasitemias of Trypanosoma spp. in Thrichomys fosteri (Rodentia: Echimyidae) in the southern Pantanal

et al. 2022

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Identification of novel mammalian hosts and Brazilian biome geographic distribution of Trypanosoma cruzi TcIII and TcIV

Cited by 24 publications

References 49 publications

A lineage-specific rapid diagnostic test (Chagas Sero K-SeT) identifies Brazilian Trypanosoma cruzi II/V/VI reservoir hosts among diverse mammalian orders

A lineage-specific rapid diagnostic test (Chagas Sero K-SeT) identifies Brazilian Trypanosoma cruzi II/V/VI reservoir hosts among diverse mammalian orders

Trypanosomatids in Small Mammals of an Agroecosystem in Central Brazil: Another Piece in the Puzzle of Parasite Transmission in an Anthropogenic Landscape

The influence of abiotic and biotic variables on the patent parasitemias of Trypanosoma spp. in Thrichomys fosteri (Rodentia: Echimyidae) in the southern Pantanal

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