Identification of novel potent pancreatic lipase inhibitors from Ficus racemosa

Abstract

Introduction and Aim: Obesity is a disorder of lipid metabolism and continues to be a global problem, ranking fifth for deaths worldwide. It is considered to be main reason for a number of physiological changes that resulting in many metabolic disorders such as cardiovascular diseases, diabetes, musculoskeletal disorders and various cancer types. Obesity develops from long-termed physiological imbalances in energy expenditure.. One of the therapeutic strategies in managing obesity is inhibition of pancreatic lipase, a key enzyme responsible for the digestion of fats and triglycerides. The aim of the present study was to synthesize silver nanoparticles (Ag-NP) from Ficus racemosa fruit extract and to analyze its activity on pancreatic lipase enzyme using spectroscopic, in vitro and in silico methods.
 Materials and Methods: The phytoconstituent were separated in Ficus racemosa fruit extract by thin layer chromatography technique. The bioactive compound was also identified by gas chromatography-mass spectrometry analysis. Pancreatic lipase inhibition assay was performed in vitro and confirmed by molecular docking analysis. 
 Results: The F. racemosa fruit showed the highest pancreatic lipase inhibitory activity. Molecular docking studies also exhibited good binding affinity of compounds (Diethyl Phthalate) with pancreatic lipase enzymes. 
 Conclusion: Finally, it is concluded that further derivation of such compounds could serve as the new templates for obesity.

Activation of Nuclear Factor Kappa B (NF-kB) Signaling Pathway Through Exercise-Induced Simulated Dopamine Against Colon Cancer Cell Lines

“…The relationship between an increase in extracellular dopamine and NF-κB activation and inflammation reveals certain intracellular targets that may be exploited to discover novel targets in colon cancer cells for effective treatment strategies. Our results are concordant with the previous studies [25][26][27]. DA was shown to reduce tumor growth, metastasis, and tumor angiogenesis [25].…”

Novel multitasking gold nanoparticles biosynthesized by Cassia fistula: antifungal, anti- obesity, anti-diabetic, and anti-ulcer activities

“…The lipase inhibitory activity exhibited by CFL-AuNPs was higher than that of CFLE, which had a maximum inhibitory activity of 33.44 ± 0.93%. Similarly, Anand et al [52] reported the in vitro lipase inhibitory activities of Ficus racemosa extract. The aqueous extract showed prominent pancreatic lipase inhibitory potential in a dose-dependent manner, ranging from 12 to 46%, with maximum inhibitory activity at a concentration of 25 µg/ml.…”

In Vitro Screening of Selected Medicinal Plants for Their Anti-bacterial Efficacy Against Few Clinical Isolates

“…When bacteria develop resistance to an antibiotic, they can either horizontally or vertically transfer this trait to their offspring. These days, new resistant bacterial strains that are somewhat more deadly than the original strain have emerged as a result of the indiscriminate and illogical use of antibiotics (Chandra et al, 2017;Anand et al, 2021) Over the world, medicinal plants have been used to cure a variety of illnesses caused by bacteria. Many chemical compounds having pharmacological uses can be found in medicinal plants.…”