Identification of Novel Potential Causal Agents of Fusarium Wilt of Musa sp. AAB in Southern Mexico

Maldonado-Bonilla, Luis David; Calderón-Oropeza, Mónica Alicia; Villarruel-Ordaz, José Luis; Sánchez-Espinosa, Ana Claudia

doi:10.35248/2157-7471.10.479

Cited by 12 publications

(11 citation statements)

References 36 publications

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“…M7, were isolated from pseudo-stem and leaves of Musa spp. ABB plants with Panama disease in Southern Mexico, respectively, but no evidence robustly supported they could cause symptoms through root inoculation ( Maldonado-Bonilla et al, 2019 ). Overall, to our knowledge, Fs has not been related to any banana growth disease yet.…”

Section: Introductionmentioning

confidence: 99%

The Genome of Banana Leaf Blight Pathogen Fusarium sacchari str. FS66 Harbors Widespread Gene Transfer From Fusarium oxysporum

Cui

Peng

et al. 2021

Front. Plant Sci.

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Fusarium species have been identified as pathogens causing many different plant diseases, and here we report an emerging banana leaf blight (BLB) caused by F. sacchari (Fs) discovered in Guangdong, China. From the symptomatic tissues collected in the field, a fungal isolate was obtained, which induced similar symptoms on healthy banana seedlings after inoculation. Koch’s postulates were fulfilled after the re-isolation of the pathogen. Phylogenetic analysis on two gene segments and the whole genome sequence identified the pathogen belonging to Fs and named as Fs str. FS66. A 45.74 Mb genome of FS66 was acquired through de novo assembly using long-read sequencing data, and its contig N50 (1.97 Mb) is more than 10-fold larger than the previously available genome in the species. Based on transcriptome sequencing and ab initio gene annotation, a total of 14,486 protein-encoding genes and 418 non-coding RNAs were predicted. A total of 48 metabolite biosynthetic gene clusters including the fusaric acid biosynthesis gene cluster were predicted in silico in the FS66 genome. Comparison between FS66 and other 11 Fusarium genomes identified tens to hundreds of genes specifically gained and lost in FS66, including some previously correlated with Fusarium pathogenicity. The FS66 genome also harbors widespread gene transfer on the core chromosomes putatively from F. oxysporum species complex (FOSC), including 30 involved in Fusarium pathogenicity/virulence. This study not only reports the BLB caused by Fs, but also provides important information and clues for further understanding of the genome evolution among pathogenic Fusarium species.

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Section: Introductionmentioning

confidence: 99%

The Genome of Banana Leaf Blight Pathogen Fusarium sacchari str. FS66 Harbors Widespread Gene Transfer From Fusarium oxysporum

Cui

Peng

et al. 2021

Front. Plant Sci.

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“…cubense , and f. sp. narcissi have showed to contain SIX10 , SIX12 , and SIX13 genes [ 12 , 17 , 22 , 54 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular and Environmental Triggering Factors of Pathogenicity of Fusarium oxysporum and F. solani Isolates Involved in the Coffee Corky-Root Disease

Gamboa-Becerra

López-Lima

Villain

et al. 2021

JoF

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Coffee corky-root disease causes serious damages to coffee crop and is linked to combined infection of Fusarium spp. and root-knot nematodes Meloidogyne spp. In this study, 70 Fusarium isolates were collected from both roots of healthy coffee plants and with corky-root disease symptoms. A phylogenetic analysis, and the detection of pathogenicity SIX genes and toxigenicity Fum genes was performed for 59 F. oxysporum and 11 F. solani isolates. Based on the molecular characterization, seven F. oxysporum and three F. solani isolates were assessed for their pathogenicity on coffee seedlings under optimal watering and water stress miming root-knot nematode effect on plants. Our results revealed that a drastic increment of plant colonization capacity and pathogenicity on coffee plants of some Fusarium isolates was caused by water stress. The pathogenicity on coffee of F. solani linked to coffee corky-root disease and the presence of SIX genes in this species were demonstrated for the first time. Our study provides evidence for understanding the pathogenic basis of F. oxysporum and F. solani isolates on coffee and revealed the presence of SIX and Fum genes as one of their pathogenicity-related mechanisms. We also highlight the relevance of chlorophyll, a fluorescence as an early and high-throughput phenotyping tool in Fusarium pathogenicity studies on coffee.

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“…Macho) at the municipality of Villa de Tututepec de Melchor Ocampo, Oaxaca, Mexico (16° 08′N, 97° 36′ W) in parallel of the experiments of isolation of Fusarium spp. previously reported (Maldonado-Bonilla et al, 2019). Cut and washed tissue samples of approximately 0.5 × 0.5 cm were disinfected with 70% ethanol for 1 min, 3% sodium hypochlorite for 10 min, and then, were rinsed three times with sterile distilled water.…”

Section: Isolation and Identification Of A Novel Trichoderma Strainmentioning

confidence: 99%

“…AAB cv. 'manzano' ('plátano manzano') plants used for this assay were developed by following the in vitro micropropagation method established in our workgroup (Maldonado-Bonilla et al, 2019)a sampling was performed in symptomatic plantains growing in fields of Oaxaca, a coastal southern state of Mexico. A phylogenetic analysis based on the sequences of TEF 1-α and IGS revealed that three isolates belonged to the Fusarium oxysporum species complex, while two other isolates were identified as members of the Fusarium fujikuroi species complex.…”

Section: Assessment Of Plant Protectionmentioning

confidence: 99%

“…Out the remaining plants, 10 were reference plants inoculated with M110 and 10 plants only inoculated with water were kept as additional testing conditions. Symptoms were recorded 10 weeks after inoculation by sorting plants into 4 categories: Healthy, yellow leaves, yellow leaves with partial necrosis and necrosis in whole leaves (Maldonado-Bonilla et al, 2019). The experiment was repeated twice.…”

Section: Assessment Of Plant Protectionmentioning

confidence: 99%

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Mycoparasitic antagonism of a Trichoderma harzianum strain isolated from banana plants in Oaxaca, Mexico

2021

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Bananas are important crops in developing countries with tropical climate. In Mexico, the banana production has increased, and it must be guaranteed. The Panama disease, caused by the fungus Fusarium oxysporum f.sp. cubense threatens the current banana production, for what is necessary to implement methods to protect this crop. Fungi from genus Trichoderma are natural residents of the rhizosphere. This genus comprises mycoparasite species used to control diseases caused by phytopathogenic fungi, and also benefit plant development. In this report, we present data of the identification and characterization of the novel strain Trichoderma harzianum M110 that displays antagonism and biocontrol potential in laboratory conditions. Exploration of the rhizosphere and the endophytic microbial communities might help to identify microbes adapted to banana plants that can be incorporated in organic biological control formulations that ensure production of Fusarium-free plants and healthy fruits with export quality.

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Identification of Novel Potential Causal Agents of Fusarium Wilt of Musa sp. AAB in Southern Mexico

Cited by 12 publications

References 36 publications

The Genome of Banana Leaf Blight Pathogen Fusarium sacchari str. FS66 Harbors Widespread Gene Transfer From Fusarium oxysporum

The Genome of Banana Leaf Blight Pathogen Fusarium sacchari str. FS66 Harbors Widespread Gene Transfer From Fusarium oxysporum

Molecular and Environmental Triggering Factors of Pathogenicity of Fusarium oxysporum and F. solani Isolates Involved in the Coffee Corky-Root Disease

Mycoparasitic antagonism of a Trichoderma harzianum strain isolated from banana plants in Oaxaca, Mexico

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