2017
DOI: 10.1038/s41598-017-11097-0
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Identification of oriT and a recombination hot spot in the IncA/C plasmid backbone

Abstract: Dissemination of multiresistance has been accelerating among pathogenic bacteria in recent decades. The broad host-range conjugative plasmids of the IncA/C family are effective vehicles of resistance determinants in Gram-negative bacteria. Although more than 150 family members have been sequenced to date, their conjugation system and other functions encoded by the conserved plasmid backbone have been poorly characterized. The key cis-acting locus, the origin of transfer (oriT), has not yet been unambiguously i… Show more

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Cited by 20 publications
(44 citation statements)
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“…MobI is required for conjugative transfer of IncC plasmids and SXT/R391 integrative and conjugative elements (ICEs) as well as GIs that mimic the oriT locus of the latter but lack a mobI gene (10,40,41). In contrast, deletion of mobI in the helper element does not abolish transfer of pCloDF13 and SGI1, which possess unique oriT loci and cognate mobilization proteins MobBC for pCloDF13 and MpsAB for SGI1 (12,16,40,42). MGIVchHai6 lacks the mobilization genes mpsAB and the oriT locus that are essential for SGI1 mobilization (13,16).…”
Section: Discussionmentioning
confidence: 99%
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“…MobI is required for conjugative transfer of IncC plasmids and SXT/R391 integrative and conjugative elements (ICEs) as well as GIs that mimic the oriT locus of the latter but lack a mobI gene (10,40,41). In contrast, deletion of mobI in the helper element does not abolish transfer of pCloDF13 and SGI1, which possess unique oriT loci and cognate mobilization proteins MobBC for pCloDF13 and MpsAB for SGI1 (12,16,40,42). MGIVchHai6 lacks the mobilization genes mpsAB and the oriT locus that are essential for SGI1 mobilization (13,16).…”
Section: Discussionmentioning
confidence: 99%
“…TraI belongs to the MOB H1 family of relaxases, and together with the product of mobI C , is essential for initiation of conjugative transfer at the origin of transfer (oriT) (10,11). MobI C is responsible for recognition of the oriT locus of IncC plasmids that is located immediately upstream of mobI C (12). Unlike other transfer genes, mobI C seems to be expressed in an AcaCD-independent manner (9).…”
mentioning
confidence: 99%
“…The relaxase-like gene mob I is also required for IncC transfer ( Carraro et al, 2014b ). While the biochemical function of the gene is unknown, it is located immediately downstream of the ori T and essential for plasmid transfer, but unrelated to SGI1 mobilization ( Hegyi et al, 2017 ). In IncC plasmids, the gene eex C facilitates entry exclusion toward IncA plasmids as well as undefined incompatibility groups by recognizing tra G ( Humbert et al, 2019 ).…”
Section: Inccmentioning
confidence: 99%
“…Figure 4A)(20,57,58). Finally, none of the 8 genes of pVCR94 Sp2 that are absent inpVCR94 Sp were expressed under the control of an AcaCD-activatable promoter (Figures 2 and 4A).…”
mentioning
confidence: 96%