Identification of oxidant susceptible proteins in Salmonella Typhimurium

Apoorva, Shekhar; Behera, Pranatee; Sajjanar, Basavaraj; Mahawar, Manish

doi:10.1007/s11033-020-05328-3

Cited by 7 publications

(8 citation statements)

References 70 publications

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“…Therefore, lower 25(OH)D 3 blood concentrations measured in WLA from 25 to 69 weeks of age are supposed to be a result of a higher conversion rate of this vitamin D derivative. Nevertheless, 25(OH)D 3 plasma levels mainly reflect the dietary supply of vitamin D ( Nys and Le Roy, 2018 ) and the higher 25(OH)D 3 values measured in BLA during the laying period might be caused by the higher feed consumption detected in BLA compared to WLA ( Lieboldt et al, 2015 ; Jansen et al, 2020a ). Taken together, our results suggest a higher calcium mobilisation from bone rather than from the diet in BLA hens, while WLA hens might cover the high calcium demands through gastrointestinal digestion.…”

Section: Discussionmentioning

confidence: 99%

“…Both chicken lines go back to breeding lines of Lohmann Tierzucht GmbH (Cuxhaven, Germany) and have been maintained in a sire rotation program since 2012. Their average egg laying performance is about 320 eggs per year, the onset of laying is around the 21st week of age and reaches a maximum of 93% (BLA) to 96% (WLA) at 28 weeks of age ( Lieboldt et al, 2015 ). For further information about performance data see Jansen et al (2020a) .…”

Section: Methodsmentioning

confidence: 99%

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Influence of Age and Phylogenetic Background on Blood Parameters Associated With Bone Metabolism in Laying Hens

et al. 2021

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The high laying performance of today’s laying hens places enormous demands on their mineral metabolism. While up-to-date data are rare, the present study aimed to describe blood parameters associated with egg laying and bone metabolism during the pre-laying period, in the course of the laying period and the daily egg laying cycle. Ten to 15 laying hens of two high-performing, phylogenetically divergent lines (BLA: brown-egg layer; WLA: white-egg layer), kept in single cages were blood sampled at 17, 25, 29, 49, and 69 weeks of age. Sampling was made at 6 a.m., 10 a.m., 2 p.m. and, with the exception of week 17, 6 p.m. Blood samples were analyzed for concentrations of total and ionized calcium, inorganic phosphate (PO4), markers of bone formation (osteocalcin) and resorption [carboxyterminal crosslinked telopeptide of type I collagen (CTX-I)], 25-hydroxycholecalciferol (25(OH)D3) and estradiol-17β. In the pre-laying period (17 week), the estradiol-17β level calculated for WLA was more than twice as high as the level calculated for BLA, while no significant difference could be observed in the laying period (25 to 69 weeks). BLA hens had significantly higher total calcium concentrations at 49 weeks of age as well as up to twice as high levels of osteocalcin and 25(OH)D3 than WLA at any time of the day from 25 to 69 weeks of age. While osteocalcin, CTX-I and 25(OH)D3 concentrations were significantly higher before the onset of lay, total calcium and estradiol-17β levels significantly increased from 17 to 69 weeks of age. In contrast, PO4 values varied only slightly during the experimental period and ionized calcium was highest at 17 and 49 weeks of age and lowest around peak production (29 week). In the course of the daily egg laying cycle blood concentrations clearly reflected the stage of egg formation. Our results provide up-to-date data of bone- and egg laying-associated blood parameters of two contemporary purebred layer lines over the course of the pre- and egg-laying period and the daily egg laying cycle. Differences between brown- and white-egg layers raise questions, whether phylogenetic background determines their efficiency to cope with high calcium demands relating to egg production.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Influence of Age and Phylogenetic Background on Blood Parameters Associated With Bone Metabolism in Laying Hens

et al. 2021

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“…The derivatized samples were then resolved on 10% SDS gel and electroblotted onto the nitrocellulose membrane. Following blocking the blot was incubated in anti-DNPH antibodies and developed as mentioned in a protocol described elsewhere 55 .…”

Section: Methodsmentioning

confidence: 99%

Malate synthase contributes to the survival of Salmonella Typhimurium against nutrient and oxidative stress conditions

Sarkhel

Apoorva

Priyadarsini

et al. 2022

Sci Rep

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To survive and replicate in the host, S. Typhimurium have evolved several metabolic pathways. The glyoxylate shunt is one such pathway that can utilize acetate for the synthesis of glucose and other biomolecules. This pathway is a bypass of the TCA cycle in which CO2 generating steps are omitted. Two enzymes involved in the glyoxylate cycle are isocitrate lyase (ICL) and malate synthase (MS). We determined the contribution of MS in the survival of S. Typhimurium under carbon limiting and oxidative stress conditions. The ms gene deletion strain (∆ms strain) grew normally in LB media but failed to grow in M9 minimal media supplemented with acetate as a sole carbon source. However, the ∆ms strain showed hypersensitivity (p < 0.05) to hypochlorite. Further, ∆ms strain has been significantly more susceptible to neutrophils. Interestingly, several folds induction of ms gene was observed following incubation of S. Typhimurium with neutrophils. Further, ∆ms strain showed defective colonization in poultry spleen and liver. In short, our data demonstrate that the MS contributes to the virulence of S. Typhimurium by aiding its survival under carbon starvation and oxidative stress conditions.

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“…Fig. S4 † illustrates the FL responses of the SUCNPs towards 500 mM ClO À at different pH values (5)(6)(7)(8)(9)(10); the optimal pH value is 7.0, so it was chosen for the following analysis.…”

Section: Optimization Of Methodsmentioning

confidence: 99%

“…To date, diverse analytical approaches have been utilized for the assay of ClO − anions, such as capillary electrophoresis, 5 electrochemiluminescence, 6 electrochemistry, 7 chromatography, 8 and fluorescence. 9 Among these, the fluorescent sensor exhibits superiority in sensitivity and stability, and is regarded as a robust tool.…”

Section: Introductionmentioning

confidence: 99%

Photoluminescent sea urchin-shaped carbon-nanobranched polymers as nanoprobes for the selective and sensitive assay of hypochlorite

Zhang

Ding

2021

RSC Adv.

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Identification of oxidant susceptible proteins in Salmonella Typhimurium

Cited by 7 publications

References 70 publications

Influence of Age and Phylogenetic Background on Blood Parameters Associated With Bone Metabolism in Laying Hens

Influence of Age and Phylogenetic Background on Blood Parameters Associated With Bone Metabolism in Laying Hens

Malate synthase contributes to the survival of Salmonella Typhimurium against nutrient and oxidative stress conditions

Photoluminescent sea urchin-shaped carbon-nanobranched polymers as nanoprobes for the selective and sensitive assay of hypochlorite

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