Identification of Pathogenic Leptospira spp. Carrier Goats  Using Polymerase Chain Reaction (PCR)

Vihol, Priti D.; Patel, Jatin H.; Patel, Jignesh M.; Dabas, V S; Kalyani, I. H.; Chaudhari, C. F.; Patel, A. D.

doi:10.20546/ijcmas.2017.612.249

Cited by 4 publications

(3 citation statements)

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“…Goats and sheep which are kept around homesteads had higher leptospiral antibodies prevalence (62%), nearly double of the 38% reported in the same species in humid tropical regions of Tanzania 21 . While, in India, 20.89% of goats 22 , and in the Virgin Islands of the USA, 11.1% of goats and 18.2% of sheep were positive by MAT 23 . Most of the studies on leptospiral infection in the world focused on the serological surveys.…”

Section: Discussionmentioning

confidence: 94%

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The role of small ruminants in the epidemiology of leptospirosis

Hajikolaei

Rezaei

Mashhadi

et al. 2022

Sci Rep

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Leptospirosis is a common global zoonotic disease of man and all farm animals. Although most leptospiral infections in sheep and goats are asymptomatic, they may play a role in the epidemiology of the disease by the spread of Leptospira through the urine. This study was carried out to evaluate the role of sheep and goats in the epidemiology of leptospirosis. Blood and urine samples were taken from 210 goats and 246 sheep. To detect antibodies, sera samples were tested with 8 live serovars of L. interrogans (Hardjo, Pomona, Grippotyphosa, Canicola, Ballum, Icterhemorrhagiae, Tarasovi, and Australis) by MAT. Then, urine samples were tested by Nested PCR targeting 16S rRNA gene for detection of pathogenic Leptospira. Results of MAT showed that 10.95% of goats and 8.53% of sheep had antibodies against at least one examined serovars. In both species, the highest reacting was L. i. Pomona with a rate of 68.18% and 56% in sheep and goats, respectively. Moreover, in PCR, 2 (0.95%) urine samples of goat and 12 (4.87%) urine samples of sheep were positive. All of the MAT positive studied animals were PCR negative and, statistical analysis showed that there was no relationship and agreement between the results of PCR and MAT in sheep (kappa = − 0.07, p > 0.05) and goats (kappa = − 0.02, p > 0.05). Finally, it is concluded that sheep and goats can excrete L. interrogans in the urine and thus transmit them to other animals and humans.

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Section: Discussionmentioning

confidence: 94%

“…Because seroreactivity was noted in 61 goats while leptospiral DNA was detected in 42 goats. Only 18 were positive in both tests and 24 seronegative were positive in PCR 22 . In the study of Cranford et al, 6.3% of sheep and 6.3% of goats were positive by rt-PCR 23 .…”

Section: Discussionmentioning

confidence: 95%

The role of small ruminants in the epidemiology of leptospirosis

Hajikolaei

Rezaei

Mashhadi

et al. 2022

Sci Rep

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“…Human and domestic animals like cattle, buffalo, goat and sheep are infected frequently over the year. Incidence and prevalence studies are reported earlier in this region from human and domestic animals [17][18][19][20][21][22][23][24][25]. However, prevalence was not studied from dogs in Gujarat.…”

Section: Introductionmentioning

confidence: 99%

Detection and Prevalence of Canine Leptospirosis from Navsari District of South Gujarat, India

Desai

Makwana

Solanki

et al. 2020

MRJI

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Aims: Leptospirosis is endemically prevalent in coastal area of south Gujarat. Generally, pets are vaccinated against Leptospira interrogans but whosoever left unvaccinated, are prone to infection. The present study was conducted to detect canine leptospirosis and its prevalence from south Gujarat. Place and Duration of Study: A total of 46 serum samples and 33 urine samples were collected from 56 dogs suspected of having leptospira infection. The study was done from September 2019 to May 2020. Methodology: Serum samples were diagnosed by IgG based ImmunoComb solid phase dot ELISA test for antibody against genus specific Leptospira interrogans. Urine samples were visualized under dark field microscopy (DFM) following laboratory manual on leptospirosis of WHO. Sex and age of dogs were noted to find out the percent positivity and prevalence. Chi square test was applied to find out any significant difference among the age groups. Results: Out of 46 serum samples, 17 (36.95%) found positive by dot ELISA and out of 33 urine sample, 13 (39.39%) found positive by DFM. Sex wise prevalence found 42.10 percent (16/38) in Male while 55.55 percent (10/18) in female dogs. Age group wise prevalence was recorded 63.63 percent (07/11) in 1- ≤ 3 years group. 27.77 per cent (05/18) in > 3- ≤ 6 years group whereas, 51.85 percent (14/27) in > 6- ≤ 12 years group. Conclusion: Detection and prevalence of canine leptospirosis were studied first time from Gujarat. Overall 46.42 percent (26/56) dogs were diagnosed positive for canine leptospirosis. Dogs having age group of 1- ≤ 3 years found more susceptible. Prevalence was found more in female than male dogs.

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