Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism

Vejl, P.; Skupinová, S.; Sedlák, Petr; Domkářová, J.

doi:10.17221/4400-pse

Cited by 11 publications

(11 citation statements)

References 7 publications

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“…Only by SSRs (microsatellite) analysis was it possible to identify different T. repens populations, even if this method expects to test a number of primer pairs and select one or several primer pairs that generate binding patterns representative for each population. The study of variability of ITS of rDNA cistron -genetically conserved source of interspecific variability is a suitable method for distinguishing different species, ecotypes and pathotypes (Vejl et al 2002). PCR-RFLP method can improve DNA analysis, but 14), 100 bp DNA ladder 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M…”

Section: This Paper Is Intent On the Completed Methodology Of New Culmentioning

confidence: 99%

Identification of white clover (Trifolium repens L.) cultivars using molecular markers

Dolanská¹,

Čurn²

2004

PLANT SOIL ENVIRON

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The different molecular analysis for specification of white clover (Trifolium repens L.) populations was studied between 2002 and 2003. RAPD, SSR (microsatellites), rDNA and PCR-RFLP markers were used for this study. The high genetic variation was detected among the cultivars but also within the cultivars by RAPD markers. For this reason RAPD markers were not found as a suitable marker system for determination of white clover cultivars. The distribution of low genetic variation of rDNA and PCR-RFLP markers was not able to differentiate cultivars. SSR and rDNA markers did not show variability of pa�erns within one cultivar. The different sizes of PCR fragments were obtained a�er amplification with microsatellite primers. SSR markers are therefore suggested as the suitable markers for the identification of different T. repens cultivars.

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Section: This Paper Is Intent On the Completed Methodology Of New Culmentioning

confidence: 99%

Identification of white clover (Trifolium repens L.) cultivars using molecular markers

Dolanská¹,

Čurn²

2004

PLANT SOIL ENVIRON

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“…Во всех случаях обычно используют праймеры, разработанные к последовательностям участков кластера генов рибосомных РНК. Методы идентификации G. rostochiensis и G. pallida, основанные на рестриктном анализе продуктов амплификации фрагментов ITS1 или ITS1+5.8S+ITS2 (Thiéry, Mugniery, 1996;Fleming et al, 1998), сменились более удобными методами ПЦР с видоспецифичными праймерами (Bulman, Marshall, 1997;Zouhar et al, 2000;Vejl et al, 2002).…”

Section: молекулярные методы идентификации видов цкнunclassified

Quarantine nematode species and pathotypes potentially dangerous for domestic potato production: populations diversity and the genetics of potato resistance

et al. 2020

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The review considers quarantine species and nematode pathotypes potentially dangerous for domestic potato production. Potatoes are affected by more than 30 types of parasitic nematodes, but the review focuses on the most harmful representatives of genera that cause great damage to potato production: Globodera, Ditylenchus, Nacob bus and Meloidogyne. Phytopathological and molecular methods of identification of species and pathotypes and the main achievements in studying the population variability of parasitic potato nematodes were analyzed. It was shown that due to the peculiarities of the life cycle of nematodes and lability of their genomes, the genetic variability of these organisms is very high, which creates a threat of forming new pathogenic genotypes of the parasites. The information about the intra- and interpopulation variability of nematodes is important for studying the ways of introduction and distribution of separate species, as well as for searching for the correlations of molecular markers with the pathotype. Phylogenetic studies based on modern data on genetic variability of populations have allowed to reveal species complexes in Globodera pallida (Stone) Behrens and Nacobbus aberrans (Thorne) Thorne & Allen (sensu lato), including cryptic species. The main components of successful protection preventing a wide distribution of parasitic nematodes are quarantine measures, agricultural techniques, biological methods of protection and cultivation of resistant cultivars. Special attention in the review is paid to the breeding of potato cultivars with durable resistance to various nematode pathotypes, because the cultivation of such varieties is the most ecologically safe and economically advantageous way to prevent epiphytoties. Currently, significant progress has been made in the genetic protection of potato cultivars, especially against cyst-forming nematodes. The review provides data on sources of potato resistance to parasitic nematodes identified in collections of wild and cultivated species. Data on identified R-gens and QTL of resistance that have been introduced into breeding varieties using different methods and approaches are analyzed. The literature data on the study of structural and functional organization of genes for resistance to potato cyst nematodes are given. The results of molecular research on revealing the polymorphisms of loci involved in the control of resistance to cyst and gall nematodes, the development of molecular markers of certain genes and their use in marker-assisted selection for developing of new resistant cultivars, including those with group resistance, are considered.

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“…Species-specific sets of primers were designed for ITS-1 amplification on the basis of known sequences of ITS-1 for both PCN species by using freeware primers. By using set of Fro1-Rro1, the product 411 bp was detected only G. rostochiensis and by using of set Fpa2-Rpa1, the product 239 bp was detected only G. pallida (Vejl et al 2002). Sequence of partial 18S, ITS1, 5.8S, ITS2 and partial 28S sequence from G. rostochiensis and G. pallida were obtained and compared to the sequence obtained from the PCR of Ukrainian population.…”

Section: Aflp/rflp Analysis Based Characterizationmentioning

confidence: 99%

Techniques for characterization and eradication of potato cyst nematode: a review

et al. 2017

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Correct identification of species and pathotypes is must for eradication of potato cyst nematodes (PCN). The identification of PCN species after completing the life cycle is very difficult because it is based on morphological and morphometrical characteristics. Genetically different populations of PCN are morphologically same and differentiated based on the host differential study. Later on these traditional techniques have been replaced by biochemical techniques viz, one and two dimensional gel electrophoresis, capillary gel electrophoresis, isozymes, dot blot hybridization and isoelectric focusing etc. to distinguish both the species. One and two dimensional gel electrophoresis has used to examine inter- and intra-specific differences in proteins of and. Now application of PCR and DNA based characterization techniques like RAPD, AFLP and RFLP are the important tools for differentiating inter- and intra specific variation in PCN and has given opportunities to accurate identification of PCN. For managing the PCN, till now we are following integrated pest management (IPM) strategies, however these strategies are not effective to eradicate the PCN. Therefore to eradicate the PCN we need noval management practices like RNAi (RNA interference) or Gene silencing.

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Identification of PCN species (Globodera rostochiensis, G. pallida) by using of ITS-1 region's polymorphism

Cited by 11 publications

References 7 publications

Identification of white clover (Trifolium repens L.) cultivars using molecular markers

Identification of white clover (Trifolium repens L.) cultivars using molecular markers

Quarantine nematode species and pathotypes potentially dangerous for domestic potato production: populations diversity and the genetics of potato resistance

Techniques for characterization and eradication of potato cyst nematode: a review

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