Identification of peptides from edible silkworm pupae (Bombyx mori) protein hydrolysates with antioxidant activity

Cermeño, Maria; Bascón, Carmen; Amigo‐Benavent, Miryam; Félix, Manuel; Fitzgerald, Richard J.

doi:10.1016/j.jff.2022.105052

Cited by 47 publications

(35 citation statements)

References 36 publications

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“…Nowadays, a variety of peptides and polyphenols with antioxidant effects are isolated and extracted from silkworm pupae by different methods, and in vitro experiments have shown that these substances are active in scavenging DPPH and ABTS free radicals and scavenging intracellularly generated ROS [ 47 , 48 , 50 ]. A study found that two peptides extracted from silkworm pupae exhibited strong antioxidant activity in HepG2 cells, as evidenced by ROS reduction, superoxide dismutase (SOD) expression, and glutathione (GSH) production activity [ 48 ]. In DPPH and ABTS radical scavenging assays, a 30% ethanolic extract of silkworm pupae was found to have the highest antioxidant activity.…”

Section: Pharmacological Functions and Mechanisms Of Silkworm Pupaementioning

confidence: 99%

Silkworm Pupae: A Functional Food with Health Benefits for Humans

Zhou

Duan

et al. 2022

Foods

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Silkworm pupae are insects that are beneficial to human health, not only for their high nutritional value but, more importantly, for the variety of pharmacological functions they can perform when consumed. Currently, there is a lot of interest in the pharmaceutical applications of silkworm pupae. In recent years, the biological functions of domestic silkworm pupae have gradually been identified and confirmed, especially for their beneficial effects on human health. Studies have found that silkworm pupae have positive effects on liver protection, immune enhancement, antiapoptosis, antitumour, antibacterial, regulation of blood glucose and blood lipids, and lowering of blood pressure. However, the pharmacological mechanisms and systemic safety of silkworm pupae have not been systematically evaluated. In this paper, the nutritional composition of the pupae of the domestic silkworm is first summarised. The pharmacological functions of silkworm pupae and their components are then classified, and their mechanisms of occurrence are described. In addition, we provide a preliminary evaluation of the safety of silkworm pupae, analyse their application prospects, and suggest future directions for further pharmacological function studies. The aim is to generate interest in the promotion of human health through the use of silkworm pupae.

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Section: Pharmacological Functions and Mechanisms Of Silkworm Pupaementioning

confidence: 99%

Silkworm Pupae: A Functional Food with Health Benefits for Humans

Zhou

Duan

et al. 2022

Foods

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“…Peptide identification was carried out using the methodology described previously. , For peptide identification, MS/MS data was analyzed using Peaks Studio 8.5 (Bioinformatics Solutions Inc., Ontario, Canada) against an in-house database using the proteomes of several species, that is, P. vulgata, Mytilus edulis (blue mussel), Lottia gigantea (owl limpet), Mizuhopecten yessoensis (scallop), and Mytilus coruscus (Korean mussel) obtained from the Uniprot database.…”

Section: Methodsmentioning

confidence: 99%

Extraction and Characterization of Protein Concentrates from Limpets (Patella vulgata) and Peptide Release Following Gastrointestinal Digestion

Bilir

Khalesi

Cermeño

et al. 2022

J. Agric. Food Chem.

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This study investigated the characterization of proteins from the Irish limpet (Patella vulgata) and assessed the in vitro biological activities of hydrolysates obtained following gastrointestinal digestion (INFOGEST) of a limpet protein concentrate (LPC). The physicochemical properties and the digestibility of the LPC were investigated, along with the angiotensin-converting enzyme (ACE) inhibition and antioxidant activities of the LPC-digested samples. All the digested samples examined outperformed the LPC in terms of activity. Peptides were identified using LC–MS/MS after digestion. A total of 38 and 19 peptides were identified in LPC-G and LPC-GI, respectively, using a database search and a de novo approach. Most of the identified peptides had hydrophobic amino acids, which may contribute to their antioxidant and ACE inhibitory activities. The findings of this study showed that LPC has high nutritional quality with good digestibility and could serve as a potential source of antioxidative and ACE inhibitory peptides following gastrointestinal digestion.

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“…Many polyphenols and peptides with antioxidant properties are now found in silkworm pupae and extracted using a variety of methods. These substances are effective at scavenging ABTS (2,2azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and DPPH (2,2diphenyl-1-picrylhydrazyl) free radicals as well as intracellularly generated ROS (Reactive oxygen species), according to in vitro tests (77)(78)(79). According to studies (78) two peptides from silkworm pupae have significant antioxidant function in HepG2 cells as evidenced by superoxide dismutase expression, ROS reduction and glutathione (GSH) production.…”

Section: Silkworm Pupae As Antioxidantmentioning

confidence: 99%

“…These substances are effective at scavenging ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and DPPH (2,2-diphenyl-1-picrylhydrazyl) free radicals as well as intracellularly generated ROS (Reactive oxygen species), according to in vitro tests ( 77 – 79 ). According to studies ( 78 ) two peptides from silkworm pupae have significant antioxidant function in HepG2 cells as evidenced by superoxide dismutase expression, ROS reduction and glutathione (GSH) production. In DPPH and ABTS radical scavenging tests, a 30% ethanol extract of pupae was discovered to possess the highest antioxidant activity.…”

Section: Silkworm Pupae Compositionsmentioning

confidence: 99%

Nutritional aspects and dietary benefits of “Silkworms”: Current scenario and future outlook

Mahanta

Komal²,

Samal³

et al. 2023

Front. Nutr.

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In the current scenario, it is estimated that by 2050, there will be an additional 2.5 billion people and a 70% increase in food demand. Crop yields are not increasing fast enough to support global needs, and world agriculture is facing several serious challenges. Therefore, insects can be a nutritious alternative to meet the ever-increasing food demand in the present and future. The majority of insect consumption occurs in developing countries, with approximately 1,900 insect species consumed worldwide. Food and feed derived from them are of high quality, have a high feed conversion ratio and emit a low level of greenhouse gases. Among insects silkworms are beneficial to humans, not only because of their high nutritional value, but also because of their several pharmacological properties. Silkworm eggs, larvae, and pupae contains high amount of proteins, oils, minerals, vitamins, and several other beneficial components which are nutritious as well as have positive effect on human health. Studies have shown that silkworm pupae protect the liver, enhance immunity, inhibit apoptosis, inhibit cancer, inhibit tumor growth, inhibit microbial growth, regulate blood glucose and blood lipids, and lower blood pressure. This review paper summerized the nutritional value of different life stages of silkworm, nutritional comparison of silkworm with the major human foods, and the effects of silkworm consumption on human health, thus ittargets to generate interest toward in sericulture and improve human health by using silkworm as a nutritious food and attain sustainability in food and nutritional security.

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Identification of peptides from edible silkworm pupae (Bombyx mori) protein hydrolysates with antioxidant activity

Cited by 47 publications

References 36 publications

Silkworm Pupae: A Functional Food with Health Benefits for Humans

Silkworm Pupae: A Functional Food with Health Benefits for Humans

Extraction and Characterization of Protein Concentrates from Limpets (Patella vulgata) and Peptide Release Following Gastrointestinal Digestion

Nutritional aspects and dietary benefits of “Silkworms”: Current scenario and future outlook

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