Identification of periodontopathogen microorganisms by PCR technique

Abstract

The identification of microorganisms by multiplex PCR is specific and sensitive. Rapid and precise assessment of different types of periodontopathogens is extremely important for early detection of the infection and consequently for the prevention and treatment of periodontal disease. In everyday clinical practice, for routine bacterial evaluation in patients with periodontal disease, the dental plaque is the most suitable biological material, because it is the richest in periodontal bacteria.

Does the Prevalence of Periodontal Pathogens Change in Elderly Edentulous Patients after Complete Denture Treatment?

“…The analysis of maxillary tuberosity swabs before and after treatment with dental prostheses has established a substantial increase of bacterial presence. As previously stated, these microorganisms are frequently implicated in multiresistant, systemic, oral, or nosocomial infections . Pathways that help their colonization and propagation should, therefore, be recognized.…”

“…As previously stated, these microorganisms are frequently implicated in multiresistant, systemic, oral, or nosocomial infections. [11][12][13][14][15][16][17][18][19] Pathways that help their colonization and propagation should, therefore, be recognized.…”

“…Numerous studies have established the relationship between periodontal pathogens and the development and progression of periodontal disease . There is also growing evidence connecting these bacteria with serious systemic diseases and conditions such as bacterial endocarditis, aspiration pneumonia (which in elderly and bedridden patients is often fatal), coronary heart disease (especially atherosclerosis), and cancer .…”

“…9,10 Numerous studies have established the relationship between periodontal pathogens and the development and progression of periodontal disease. [11][12][13] There is also growing evidence connecting these bacteria with serious systemic diseases and conditions such as bacterial endocarditis, 14 aspiration pneumonia (which in elderly and bedridden patients is often fatal), 15,16 coronary heart disease (especially atherosclerosis), 17 and cancer. 18 Though these diseases can affect any age group, elderly patients have been reported to be more subject to bacterial infection and systemic complication due to the reduced immune response.…”

Periodontal Inflammation as Risk Factor for Pancreatic Diseases

“…The disease is a chronic low grade infection involving mainly Gram-negative anaerobic bacteria. There is a clear evidence for the pathogenic role of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis and Tannerella forsythia in periodontal disease development and a reasonably strong evidence exists for the pathogenic role in certain forms of periodontal disease of some other microorganisms such as Prevotella intermedia, Fusobacterium nucleatum, Campylobacter rectus, Eikenella corrodens etc (Brajovic et al, 2010;Milicevic et al, 2008). A number of gram-negative rods and spirochetes are putative periodontal pathogens, but these organisms may also be present, although in smaller concentrations, in healthy patients.…”