Identification of Peste des Petits Ruminants Virus, Georgia, 2016

Donduashvili, Marina; Goginashvili, Ketevan; Toklikishvili, Natela; Tigilauri, Tamar; Gelashvili, Lamara; Avaliani, Lasha; Khartskhia, Natia; Loitsch, Angelika; Bataillé, Arnaud; Libeau, Geneviève; Diallo, Adama; Dundon, William G.

doi:10.3201/eid2408.170334

Cited by 30 publications

(28 citation statements)

References 9 publications

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“…In summary, the results of this study indicate that the PPRV involved in the reported outbreak in Burundi is related to the PPRV lineage III circulating in the East Africa region. Transboundary movements of infected animals might be at the origin of this outbreak, as has been described in several countries both in Asia (Muthuchelvan et al, ; Shatar et al, ) and Africa (Boussini et al, ; Dundon et al, ) although not always with clear explanations (Donduashvili et al, ). The control and regulation of such animal movements will assist in the control and management of PPRV as attempts are made to eradicate the disease globally by 2030.…”

Section: Resultsmentioning

confidence: 76%

“…Peste des Petits Puminants (PPR) is a highly contagious viral disease of small ruminants which was first described in Côte d'Ivoire (Gargadennec & Lalanne, ) and has now been reported in many parts of Africa, the Middle East, Eurasia (Bulgaria, Georgia and Turkey) and Asia (Donduashvili et al, ; Libeau, Diallo, & Parida, ; Parida et al, ). The PPR virus (PPRV) belongs to the genus Morbillivirus within the Paramyxoviridae family (Gibbs, Taylor, Lawman, & Bryant, ) and causes a disease which is characterized by high fever, ocular‐nasal discharge, stomatitis, diarrhoea and pneumonia.…”

Section: Introductionmentioning

confidence: 99%

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Detection and molecular characterization of Peste des Petits Ruminants virus from outbreaks in Burundi, December 2017–January 2018

Alfred

Baziki

Dundon

et al. 2019

Transbound Emerg Dis

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In December 2017, Peste des Petits Ruminants (PPR) emerged in Burundi (East Africa) and rapidly spread to five provinces (Gitega, Kirundo, Mwaro, Muramvya and Karuzi) in the country, causing severe disease and killing more than 4,000 goats in the province of Gitega alone. An initial outbreak investigation was conducted in December 2017 by the Burundi Government Veterinary Services and samples were collected for laboratory confirmation. A competitive Enzyme Linked Immuno‐Sorbent Assay (cELISA: Chinese Patent No. ZL201210278970.9) supplied by the Lanzhou Veterinary Research Institute was used to test 112 sera and results showed around 37.5% positive samples. This high level of PPR positive sera in an animal population where PPR infection and vaccination had not been previously reported indicated the exposure of the animals to PPRV. Subsequently in January 2018, the laboratory tests conducted at the African Union‐Pan African Veterinary Vaccine Centre (AU‐PANVAC) laboratories following a joint investigative mission by the African Union‐Interafrican Bureau for Animal Resources (AU‐IBAR), AU‐PANVAC and the East African Community (EAC) confirmed the presence of PPR in Burundi. Samples tested by conventional RT‐PCR indicated the presence of the PPR virus (PPRV). Confirmatory isolation of the virus was also performed. Phylogenetic analysis revealed that the virus belongs to lineage III and shows a close relationship with PPRV isolates from Kenya in 2011 and Uganda in 2012. A possible explanation for the outbreaks of PPR in Burundi between December 2017 and February 2018 is presented.

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Section: Resultsmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Detection and molecular characterization of Peste des Petits Ruminants virus from outbreaks in Burundi, December 2017–January 2018

Alfred

Baziki

Dundon

et al. 2019

Transbound Emerg Dis

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“…PPR was first reported in 1942 in Ivory Coast, West Africa [ 6 ]. Since then, it has spread across northern and sub-Saharan Africa into Asia and has now been reported in over 70 countries in Africa, the Near and Middle East, and Central and Eastern Asia [ 7 , 8 , 9 , 10 , 11 , 12 , 13 ], including Georgia in 2016 [ 14 ] and Bulgaria in 2018 [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

A Novel Recombinant Newcastle Disease Virus Vectored DIVA Vaccine against Peste des Petits Ruminants in Goats

et al. 2020

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Peste des petits ruminants virus (PPRV, species: small ruminant morbillivirus) is the causative agent of the eponymous notifiable disease, the peste des petits ruminants (PPR) in wild and domestic sheep and goats. Mortality rates vary between 50% and 100%, causing significant losses of estimated 1.5 to 2 billion US Dollars per year. Live-attenuated PPRV vaccine strains are used in the field for disease prevention, but the application of a more thermostable vaccine enabling differentiation between infected and vaccinated animals (DIVA) would be highly desirable to achieve the goal of global disease eradication. We generated a recombinant Newcastle disease virus (rNDV) based on the live-attenuated NDV Clone 30 that expresses the surface protein hemagglutinin (H) of PPRV strain Kurdistan/11 (rNDV_HKur). In vitro analyses confirmed transgene expression as well as virus replication in avian, caprine, and ovine cells. Two consecutive subcutaneous vaccinations of German domestic goats with rNDV_HKur prevented clinical signs and hematogenic dissemination after an intranasal challenge with virulent PPRV Kurdistan/11. Virus shedding by different routes was reduced to a similar extent as after vaccination with the live-attenuated PPRV strain Nigeria 75/1. Goats that were either not vaccinated or inoculated with parental rNDV were used as controls. In summary, we demonstrate in a proof-of-concept study that an NDV vectored vaccine can protect against PPR. Furthermore, it provides DIVA-applicability and a high thermal tolerance.

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“…PPRV was first reported in Ivory Coast (Cote d'Ivoire) of West Africa in 1942 (8). Now, the disease has spread in Central and East Africa, the Middle East, Turkey, China, India, and Nepal, reaching Europe's doorstep with cases reported in Morocco (9), Turkey (10), and Georgia (11). If left uncontrolled, it will spread even further, causing massive loss and hardship for millions of farmers and herders.…”

Section: Introductionmentioning

confidence: 99%

Development of an Enzyme-Linked Immunosorbent Assay Based on CD150/SLAM for the Detection of Peste des Petits Ruminant Virus

et al. 2020

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Peste des petits ruminant (PPR) is an economically important severe viral disease of small ruminants that affects primarily the respiratory and digestive tract. Specific detection of the PPR virus (PPRV) antigen plays an important role in the disease control and eradication program. In this study, an indirect enzyme-linked immunosorbent assay (ELISA) based on the recombinant goat signaling lymphocyte activation molecule (SLAM) as the capture ligand was successfully developed for the detection of the PPRV antigen (PPRV SLAM-iELISA). The assay was highly specific for PPRV with no cross-reactions among foot and mouth disease virus, Orf virus, sheep pox virus, and goat pox virus and had a sensitivity with a detection limit of 1.56 × 10 1 TCID 50 /reaction (50 µl). Assessment of 136 samples showed that the developed PPRV SLAM-iELISA was well correlated with real-time RT-qPCR assays and commercially available sandwich ELISA for detection of PPRV and showed relative sensitivity and specificity of 93.75 and 100.83%, respectively. These results suggest that the developed PPRV SLAM-iELISA is suitable for specific detection of the PPRV antigen. This study demonstrated for the first time that the goat SLAM, the cellular receptor for PPRV, can be used for the development of a diagnostic method for the detection of PPRV.

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Identification of Peste des Petits Ruminants Virus, Georgia, 2016

Cited by 30 publications

References 9 publications

Detection and molecular characterization of Peste des Petits Ruminants virus from outbreaks in Burundi, December 2017–January 2018

Detection and molecular characterization of Peste des Petits Ruminants virus from outbreaks in Burundi, December 2017–January 2018

A Novel Recombinant Newcastle Disease Virus Vectored DIVA Vaccine against Peste des Petits Ruminants in Goats

Development of an Enzyme-Linked Immunosorbent Assay Based on CD150/SLAM for the Detection of Peste des Petits Ruminant Virus

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