Identification of phenolic compounds from pomegranate (Punica granatum L.) seed residues and investigation into their antioxidant capacities by HPLC–ABTS+ assay

He, Li; Xu, Huaide; Liu, Xuan; He, Wenhao; Yuan, Fang; Hou, Zhanqun; Gao, Yanxiang

doi:10.1016/j.foodres.2010.05.023

Cited by 116 publications

(86 citation statements)

References 44 publications

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“…Then, the supernatant was fractionized into four fractions with ethyl ether, ethyl acetate and n-butanol, respectively (He et al 2011). The upper phase during each liquid-liquid extraction was marked as Fa, Fb and Fc.…”

Section: Preparation Of the Crude Extract And Fractionsmentioning

confidence: 99%

“…Seperation of the antioxidant compounds in four fractions of the extract was performed on a HPLC equipment of Agilent 1100 series with a diode array detector (DAD) (He et al 2011). Sample (20 μL) was separated on an Agilent Zorbax SB C-18 comlumn (250 mm×4.6 mm i.d., 5 μm particle size) with a gradient elution system.…”

Section: Hplc-dad and Hplc-edi-ms N Analysismentioning

confidence: 99%

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Extraction and analysis of antioxidant compounds from the residues of Asparagus officinalis L.

et al. 2014

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Asparagus residues were used as materials to obtain antioxidant compounds by solid-liquid extraction in this study. The effects of different extraction parameters including extraction solvents, time, temperature and liquid-solid ratio on the contents of total flavonoids, total phenolics and total antioxidant activity were investigated. Antioxidant activity of the extract from asparagus residues was evaluated by HPLC-ABTS· + and the bioactive components were identified by HPLC-MS/MS. The results showed that the extraction yield was significantly influenced (P<0.05) by solvent composition, extraction time and temperature. The appropriate parameters were preferred as extraction solvent of 50 % ethanol with liquid-solid ratio of 30:1, extraction temperature of 80°C and time of 2 h. Antioxidant activity evaluation of the extract indicated flavonoids and phenolics were dominant bioactive compounds. Five antioxidant compounds were identified as ferulic acid, kaempferol, quercetin, rutin and isorhamnetin.

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Section: Preparation Of the Crude Extract And Fractionsmentioning

confidence: 99%

Section: Hplc-dad and Hplc-edi-ms N Analysismentioning

confidence: 99%

Extraction and analysis of antioxidant compounds from the residues of Asparagus officinalis L.

et al. 2014

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“…A técnica de cromatografia líquida de alta eficiência acoplada a detecção da captura de radicais livres por compostos individuais pós-coluna baseado nos sistemas modelos de oxidação têm sido aplicada em diversos estudos científicos (HE et al, 2011;HE et al, 2012;BORGES et al, 2010;SANTIAGO et al, 2014;GULLON et al, 2016). Constitui um método sensível e relativamente simples para análise da capacidade antioxidante individual dos compostos, tendo sido aplicado para uma análise rápida e eficiente de matrizes complexas como extratos de plantas e de alimentos (PELLEGRINI et al, 2003;NUENGCHAMNONGETAL et al, 2005;BANDONIEN e MURKOVIC, 2002;HE et al, 2011).…”

Section: On Line Hplc-dad-abtsunclassified

“…Constitui um método sensível e relativamente simples para análise da capacidade antioxidante individual dos compostos, tendo sido aplicado para uma análise rápida e eficiente de matrizes complexas como extratos de plantas e de alimentos (PELLEGRINI et al, 2003;NUENGCHAMNONGETAL et al, 2005;BANDONIEN e MURKOVIC, 2002;HE et al, 2011). …”

Section: On Line Hplc-dad-abtsunclassified

“…A fase móvel foi constituída por uma mistura de dois solventes: (A) Solução de ácido fórmico em água a 1% e (B) Metanol. A vazão da fase móvel foi de 0,7 mL.min -1 e o gradiente foi iniciado em 0% do solvente B passando a 60% de B em 45 minutos, 100% de B em 50 minutos e retornando novamente a 0% do solvente B a 60 minutos (HE et al, 2011). As amostras e os pontos da curva padrão de Trolox foram avaliadas em três repetições.…”

Section: On Line Hplc-dad-abtsunclassified

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Efeito neuroprotetor da casca de romã (<i>Punica granatum</i>)

Morzelle¹

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Characterization of pomegranate (Punica granatum L.) seed and oils

Juhaimi

Özcan

Ghafoor

2017

Euro J Lipid Sci & Tech

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The main objective of current study was to characterize of pomegranate (Punica granatum L.) seed and oils as a source of phytochemical, and bioactive compounds. While total phenol contents of seed extracts vary between 23.6 mg gallic acid equivalent/g and 28.8 mg gallic acid equivalent/g, their antioxidant activities were changed between 17.6 and 22.9% (p < 0.05). The seeds of pomegranate varieties contained 347–647 mg/kg Ca, 2076–3846 mg/kg K, 1572–2327 mg/kg P, and 1357–1781 mg/kg Mg. While the total phenol contents of seed oils change between 7.8 mg gallic acid equivalent/g and 19.2mg GAE/g, antioxidant activity of oil samples varied between 6.8 and 8.8% (p < 0.05). The predominant fatty acid of oils is punicic acid, and punicic acid contents of oil samples were found between 71.2 and 77.6%. Oleic acid contents of seed oils were determined between 7.6 and 9.1%. In addition, ɣ‐tocopherol contents of pomegranate oil ranged from 236 mg/100 g to 389 mg/100 g. Pomegranate seeds are sources of bioactive and phytochemical such as fatty acid, ɣ‐tocopherol, total phenolic content, and flavonoids with potentially high antioxidant activities. Practical applications: In folk medicine for many centuries, pomegranate is an important source of bioactive and phytochemical compounds. Pomegranate fruit are known to posses strong antioxidant activity, and its oil consists of approximately 80% punicic acid. Pomegranate seed contains a range of nutraceutical components such as punicic acid, sterols, and ɣ‐tocopherol. Total phenol contents of seed powder extracts varied between 23.56 mg gallic acid equivalent/g (Silifke Aşı) and 28.75 mg gallic acid equivalent/g (Devedişi). In addition, antioxidant activities of pomegranate seed extracts were found between 17.56 (Erdemli Aşı) and 22.93% (Nizip). The total flavonoid contents of pomegranate seed extracts changed between 13.56 mgCE/g (Mayhoş) and 23.51 mgCE/g (Nizip).The total anthocyanin contents of seeds changed between 13.56 μmol/g (Mayhoş) and 23.51 μmol/g (Nizip). As seen in Table 2, the highest total anthocyanin content (23.51 μmol/g) was found in Nizip, followed by Silifke Aşı, Erdemli Aşı, and Hicaz varieties.

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Identification of phenolic compounds from pomegranate (Punica granatum L.) seed residues and investigation into their antioxidant capacities by HPLC–ABTS+ assay

Cited by 116 publications

References 44 publications

Extraction and analysis of antioxidant compounds from the residues of Asparagus officinalis L.

Extraction and analysis of antioxidant compounds from the residues of Asparagus officinalis L.

Efeito neuroprotetor da casca de romã (<i>Punica granatum</i>)

Characterization of pomegranate (Punica granatum L.) seed and oils

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