Identification of Potential Biomarkers for Gut Barrier Failure in Broiler Chickens

Chen, Juxing; Téllez, G.; Richards, J. D.; Escobar, Jeffery

doi:10.3389/fvets.2015.00014

Cited by 188 publications

(181 citation statements)

References 105 publications

(131 reference statements)

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“…Therefore, it is possible that Thai-breed chickens might be affected by fasting (12 h) before sample collection, which caused a reduction in the mucin intestinal surface and intestinal morphology. Different types of diets can affect the intraluminal milieu by disturbing the digestive viscosity and mucins (7). However, all chickens in our study were fed the same diet and nutrients to reduce the effect of dietary factors.…”

Section: Discussionmentioning

confidence: 99%

“…Claudin and occludin proteins are mainly responsible for intestinal barrier function and paracellular diffusion (1,7,8,19). Claudin-1 proteins are constantly expressed in the intestinal lining from the hatching period to the formation of adult chickens (20).…”

Section: Discussionmentioning

confidence: 99%

“…This can be used as an indicator for alterations in the intestinal epithelium (6). In chickens, an impaired intestinal barrier can lead to poor growth performance due to inefficient nutrient digestion and absorption (7). Several studies have noted that mRNA expression and the levels of serum proteins, e.g., claudin-1, occludin, ZO-1, and cytokines, can be used in broilers as biomarkers to determine the function of the gut barrier (7,8).…”

Section: Introductionmentioning

confidence: 99%

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Intestinal barrier and mucosal immunity in broilers, Thai Betong, and native Thai Praduhangdum chickens

Theerawatanasirikul¹,

Koomkrong²,

Kayan³

et al. 2017

Turk J Vet Anim Sci

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The intestinal barrier includes physical and chemical components for preventing the invasion of pathogenic and toxic agents. The aim of this study was to investigate small intestinal morphology, antimicrobial peptides, and tight junction (TJ) distribution among broilers, Thai Betong chickens, and native Thai Praduhangdum chickens. Intestinal samples from 40 chickens of each breed were collected. The results of the histological and morphological examination revealed that the duodenum of all breeds had the maximum villus height compared to the other parts. The intestinal tract of both Betong and Praduhangdum chickens was low in the number of mucin and goblet cells. In broilers, the intestinal surface mucins correlated with the number of β-defensin-positive Paneth cells. Claudin-1 protein was observed in the cytoplasm of the epithelium in all breeds, with the highest intensity of claudin-1 staining detected in the intestinal cells of the ileum. Localization of occludin was higher in the broilers in all intestinal segments than in the native Thai chickens. In conclusion, the broilers exhibited a large amount of TJ proteins and mucosal immunity as compared to native Thai-breed chickens. TJ localizations together with intestinal morphology and mucosal immunity can be utilized as biological markers for gut health.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Intestinal barrier and mucosal immunity in broilers, Thai Betong, and native Thai Praduhangdum chickens

Theerawatanasirikul¹,

Koomkrong²,

Kayan³

et al. 2017

Turk J Vet Anim Sci

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“…It has been demonstrated that poor gut health due to the disruption of the intestinal barrier, such as the one triggered by Eimeria, causes an undesirable impact in health and growth performance of broiler chickens (Chen et al 2015). Inflammatory responses and gut disorders in broilers have economic consequences, such as a decrease in body weight gain and overall performance of the flock (Van Leeuwen et al 2004;Shirzadi et al 2010).…”

Section: Discussionmentioning

confidence: 99%

In vivo anticoccidial activity of quinfamide in broilers: a preliminary report

Aquino

Alcalá

Gutiérrez

et al. 2016

Italian Journal of Animal Science

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The aim of this trial was to evaluate the anticoccidial efficacy of quinfamide in broilers. Three different treatments were implemented over a 49-day period as follows: quinfamide; quinfamide plus carbopol and decoquinate, all prepared as small pellets and mixed with feed at a final dose of either active principle of 30 ppm. Parameters measured were: weight gain, number of oocysts shedding per gram of litter and degree of gross lesions caused by coccidia. Body weight gain was statistically greater for quinfamide and quinfamide-carbopol groups in comparison to other groups. However, only the quinfamide-carbopol group showed similar efficacy in the occyst counts as compared to the decoquinate group. Statistically significant differences were observed when intestinal lesions score were compared and the less affected group was quinfamide-carbopol. Based on these results, it is concluded that quinfamide possesses a low anticoccidial efficacy. However, this is noticeably improved when it is prepared as pellets with carbopol. The adhesion of carbopol to intestinal mucosa may influence residence time of quinfamide in the gastrointestinal tract, thus enhancing efficacy. ARTICLE HISTORY

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“…본 실험에서 처 리구와 대조구간의 지방산패도의 유의적 차이는 나타나지 않았다(P>0.05). Aengwanich and Suttajit(2010) (Song et al, 2014;Chen et al, 2015). Fig.…”

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Effect of Dietary-Liquid Resveratrol on Growth Performance, Meat Quality Traits, and Ileum Morphology of Broiler Chickens under Moderate Heat Stress

Yi¹,

Yoo²,

Samiru³

et al. 2016

Korean Journal of Poultry Science

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A total of 144 one-day-old male broilers (ROSS × Ross 308) (42±0.1 g) were used in a completely randomized design and allotted to one of three dietary treatments to give 6 replicates per treatment (8 birds per cage). The three watery dietary treatments were 1) a corn-soybean meal-based relation [Negative control (NC); no antimicrobial compounds added] with tab water, 2) 8 ppm liquid-alpha-lipoic acid [Positive control (PC)] or 3) 100 ppm liquid-resveratrol. All dietary treatments were continuously provided as a liquid form. Birds were housed in a battery cage (n=48), and were offered liquid dietary treatments at all times. The ambient temperature was maintained at 32±1℃ for the first 3 weeks and decreased gradually to 28±1℃ by the end of the experiment (d 35) to induce moderate heat stress. One bird per pen (n=6) was euthanized via cervical dislocation at day 21 to obtain terminal ileum to measure villous height and crypt depth, while another bird per pen (n=6) was similarly euthanized at day 35 and used to harvest breast meat and drumsticks to evaluate meat quality traits. Birds fed liquid-resveratrol did not improve (P>0.05) body weight and average daily gain compared with those fed other dietary treatments from day 1 to day 35. Birds fed liquid-resveratrol had no effect (P>0.05) on villous height compared with birds were in other dietary treatments at day 21. Liquid-resveratrol had no effect (P>0.05) on thiobarbituric acid reactive substance (TBARS) in drumsticks compared with other dietary treatments at day 35. Furthermore, birds fed liquid-resveratrol had no effect (P>0.05) on DPPH radical scavenging activity both in breast meat and drumsticks compared with those fed other dietary treatments at day 35. Morphological changes of ileum were not observed by immunofluorescence, and the level of occludin protein from ileum extracts also did not differ among groups. Our results suggested that liquid form of antioxidant compounds used in the current study such as alpha-lipoic acid (8 ppm) or resveratrol (100 ppm) did not improve growth performance, meat quality traits and histology of terminal ileum compared with those fed negative control in broiler chickens under moderate heat stress for 35 days.

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Identification of Potential Biomarkers for Gut Barrier Failure in Broiler Chickens

Cited by 188 publications

References 105 publications

Intestinal barrier and mucosal immunity in broilers, Thai Betong, and native Thai Praduhangdum chickens

Intestinal barrier and mucosal immunity in broilers, Thai Betong, and native Thai Praduhangdum chickens

In vivo anticoccidial activity of quinfamide in broilers: a preliminary report

Effect of Dietary-Liquid Resveratrol on Growth Performance, Meat Quality Traits, and Ileum Morphology of Broiler Chickens under Moderate Heat Stress

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