Identification of potential endometriosis biomarkers in peritoneal fluid and blood plasma via shotgun lipidomics

Стародубцева, Н. Л.; Chagovets, Vitaliy; Borisova, Anna V.; Salimova, Dinara; Aleksandrova, N. V.; Chingin, Konstantin; Chen, Huanwen; Frankevich, Vladimir

doi:10.1016/j.clinms.2019.05.007

Cited by 15 publications

(8 citation statements)

References 24 publications

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“…Finally, on the path to considering the use of PC/LPC in a point of care setting, the utility of finger-prick blood collection as a viable option for measuring PC/LPC was considered. Given the practical considerations described above and that elevated PC in plasma and peritoneal fluid is associated strongly with endometriosis, 58 we studied the menstrual cycle as a first step. There were clear differences in PC/LPC of men and women that were cyclically determined in women.…”

Section: Discussionmentioning

confidence: 99%

The dynamic inflammatory profile of pregnancy can be monitored using a novel lipid-based mass spectrometry technique

et al. 2023

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Section: Discussionmentioning

confidence: 99%

The dynamic inflammatory profile of pregnancy can be monitored using a novel lipid-based mass spectrometry technique

et al. 2023

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“…Some studies reported lower levels of sphingomyelins in peritoneal fluid [ 72 , 73 , 103 ]. Taken together with dysregulation of the sphingosine pathway in ectopic endometrium [ 104 ], this observation implies greater oxidative stress in endometriosis lesions, because oxidative stress promotes the conversion of sphingomyelins to ceramides [ 105 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolomics for Diagnosis and Prognosis of Uterine Diseases? A Systematic Review

Tokarz

Adamski

Rižner

2020

JPM

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This systematic review analyses the contribution of metabolomics to the identification of diagnostic and prognostic biomarkers for uterine diseases. These diseases are diagnosed invasively, which entails delayed treatment and a worse clinical outcome. New options for diagnosis and prognosis are needed. PubMed, OVID, and Scopus were searched for research papers on metabolomics in physiological fluids and tissues from patients with uterine diseases. The search identified 484 records. Based on inclusion and exclusion criteria, 44 studies were included into the review. Relevant data were extracted following the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analysis) checklist and quality was assessed using the QUADOMICS tool. The selected metabolomics studies analysed plasma, serum, urine, peritoneal, endometrial, and cervico-vaginal fluid, ectopic/eutopic endometrium, and cervical tissue. In endometriosis, diagnostic models discriminated patients from healthy and infertile controls. In cervical cancer, diagnostic algorithms discriminated patients from controls, patients with good/bad prognosis, and with/without response to chemotherapy. In endometrial cancer, several models stratified patients from controls and recurrent from non-recurrent patients. Metabolomics is valuable for constructing diagnostic models. However, the majority of studies were in the discovery phase and require additional research to select reliable biomarkers for validation and translation into clinical practice. This review identifies bottlenecks that currently prevent the translation of these findings into clinical practice.

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“…The high diagnostic potential of lipidomics has been shown in many areas of medicine, particularly in oncology: lung, thyroid gland, breast, stomach, pancreas, colorectal, liver, kidney, prostate, ovarian, and endometrium cancer 29 – 52 . MS studies of lipid profiles in tissues and blood plasma have revealed new promising biomarkers of endometriosis (benign gynecological disorder) 27 , 28 , 37 , 40 , 47 , 48 , 52 . To date there are no sufficient metabolomics data for uterine fibroids, as only one study by Heinonen H. R. group was conducted in tissues 53 .…”

Section: Introductionmentioning

confidence: 99%

Alterations in lipid profile upon uterine fibroids and its recurrence

Tonoyan

Chagovets

Стародубцева

et al. 2021

Sci Rep

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Uterine fibroids (UF) is the most common (about 70% cases) type of gynecological disease, with the recurrence rate varying from 11 to 40%. Because UF has no distinct symptomatology and is often asymptomatic, the specific and sensitive diagnosis of UF as well as the assessment for the probability of UF recurrence pose considerable challenge. The aim of this study was to characterize alterations in the lipid profile of tissues associated with the first-time diagnosed UF and recurrent uterine fibroids (RUF) and to explore the potential of mass spectrometry (MS) lipidomics analysis of blood plasma samples for the sensitive and specific determination of UF and RUF with low invasiveness of analysis. MS analysis of lipid levels in the myometrium tissues, fibroids tissues and blood plasma samples was carried out on 66 patients, including 35 patients with first-time diagnosed UF and 31 patients with RUF. The control group consisted of 15 patients who underwent surgical treatment for the intrauterine septum. Fibroids and myometrium tissue samples were analyzed using direct MS approach. Blood plasma samples were analyzed using high performance liquid chromatography hyphened with mass spectrometry (HPLC/MS). MS data were processed by discriminant analysis with projection into latent structures (OPLS-DA). Significant differences were found between the first-time UF, RUF and control group in the levels of lipids involved in the metabolism of glycerophospholipids, sphingolipids, lipids with an ether bond, triglycerides and fatty acids. Significant differences between the control group and the groups with UF and RUF were found in the blood plasma levels of cholesterol esters, triacylglycerols, (lyso) phosphatidylcholines and sphingomyelins. Significant differences between the UF and RUF groups were found in the blood plasma levels of cholesterol esters, phosphotidylcholines, sphingomyelins and triacylglycerols. Diagnostic models based on the selected differential lipids using logistic regression showed sensitivity and specificity of 88% and 86% for the diagnosis of first-time UF and 95% and 79% for RUF, accordingly. This study confirms the involvement of lipids in the pathogenesis of uterine fibroids. A diagnostically significant panel of differential lipid species has been identified for the diagnosis of UF and RUF by low-invasive blood plasma analysis. The developed diagnostic models demonstrated high potential for clinical use and further research in this direction.

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Identification of potential endometriosis biomarkers in peritoneal fluid and blood plasma via shotgun lipidomics

Cited by 15 publications

References 24 publications

The dynamic inflammatory profile of pregnancy can be monitored using a novel lipid-based mass spectrometry technique

The dynamic inflammatory profile of pregnancy can be monitored using a novel lipid-based mass spectrometry technique

Metabolomics for Diagnosis and Prognosis of Uterine Diseases? A Systematic Review

Alterations in lipid profile upon uterine fibroids and its recurrence

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