Identification of potential fermentation inhibitors in conversion of hybrid poplar hydrolyzate to ethanol

Luo, Caidian; Brink, David L.; Blanch, Harvey W.

doi:10.1016/s0961-9534(01)00061-7

Cited by 177 publications

(113 citation statements)

References 15 publications

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“…Xylose and arabinose are the monomeric pentose sugars from hydrolysis of the hemicellulose fraction [9,14]. Also, glucose produced in hydrolysate is from hemicellulose or/ and cellulose fractions [15].…”

Section: Discussionmentioning

confidence: 99%

Screening of Yeasts for Selection of Potential Strains and Their Utilization for In Situ Microbial Detoxification (ISMD) of Sugarcane Bagasse Hemicellulosic Hydrolysate

et al. 2016

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Many toxic compounds are produced and released in the hemicellulosic hydrolyzates during the acid pretreatment step, which are required for the disruption of the lignocelluloses matrix and sugars release. The conventional methods of detoxification i.e. overliming, activated charcoal, ion exchange or even membrane-based separations have the limitations in removal of these toxic inhibitors in fermentation process. Hence, it is imperative to explore biological methods to overcome the inhibitors by minimizing the filtration steps, sugar loss and chemical additions. In the present study we screened sixty-four strains of yeasts to select potential strains for detoxification of furfural, acetic acid, ferulic acid, 5-hydroxymethyl furfural (5-HMF) as carbon and energy source. Among these strains Pichia occidentalis M1, Y1 0 a, Y1 0 b and Y3 0 showed a significant decrease in the toxic compounds but we selected two best yeast strains i.e. P. occidentalis Y1 0 a and P. occidentalis M1 for the further experiments with an aim to remove the fermentation inhibitors. The yeasts P. occidentalis Y1 0 a and P. occidentalis M1 were grown aerobically in sugarcane bagasse hemicellulose hydrolysate under submerged cultivation. For each yeast, a 2 2 full factorial design was performed considering the variables-pH (4.0 or 5.0) and agitation rate (100 or 300 rpm), and the percentage removal of HMF, furfural, acetic acid and phenols from hemicellulosic hydrolysates were responsive variables. After 96 h of biological treatment, P. occidentalis M1 and P. occidentalis Y1 0 a showed 42.89 and 46.04 % cumulative removal of inhibitors, respectively.

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Section: Discussionmentioning

confidence: 99%

Screening of Yeasts for Selection of Potential Strains and Their Utilization for In Situ Microbial Detoxification (ISMD) of Sugarcane Bagasse Hemicellulosic Hydrolysate

et al. 2016

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“…Villarreal et al (2006) observed also that ionexchange resins were more efficient than activated charcoal to remove all major groups of inhibitory compounds without sugar loss for detoxification of eucalyptus hemicellulose prehydrolysates. Other researchers have reported the use of ion exchange resins like one of the best methods to remove phenolics from hemicellulosic hydrolysates (Van Zyl et al, 1991;Nilvebrant et al, 2001;Luo et al, 2002;Chandel et al, 2007). …”

Section: Prehydrolysate Detoxificationmentioning

confidence: 99%

Hemicellulose-derived sugars solubilisation of rape straw. Cofermentation of pentoses and hexoses by Escherichia coli

López-Linares

Cara-Corpas

Ruiz

et al. 2015

Span. j. agric. res.

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Bioconversion of hemicellulose sugars is essential for increasing fuel ethanol yields from lignocellulosic biomass. We report for the first time with rape straw, bioethanol production from hemicellulose sugars. Rape straw was pretreated at mild conditions with sulfuric acid to solubilize the hemicellulose fraction. This pretreatment allows obtaining a prehydrolysate, consisting basically in a solution of monomeric hemicellulosic sugars, with low inhibitor concentrations. The remaining water insoluble solid constitutes a cellulose-enriched, free of extractives material. The influence of temperature (120ºC and 130ºC), acid concentration (2-4% w/v) and pretreatment time (30-180 min) on hemicellulose-derived sugars solubilisation was evaluated. The highest hemicellulosic sugars recovery, 72.3%, was achieved at 130ºC with 2% sulfuric acid and 60 min. At these conditions, a concentrated sugars solution, 52.4 g/L, was obtained after three acid consecutive contacts, with 67% xylose and acetic acid concentration above 4.5 g/L. After a detoxification step by activated charcoal or ion-exchange resin, prehydrolysate was fermented by ethanologenic Escherichia coli. An alcoholic solution of 25 g/L and 86% of theoretical ethanol yield was attained after 144 h when the prehydrolysate was detoxified by ion-exchange resin. The results obtained in the present work show sulfuric acid pretreatment under mild conditions and E. coli as an interesting process to exploit hemicellulosic sugars in rape straw.Additional key words: agricultural residue; acid pretreatment; xylose; E. coli; bioethanol production

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“…Note that data in Figure 1 were collected over 2 min. Compared with traditional chromatographic separations of biomass hydrolysates [6,10,13,45], a 2-min analysis time represents a 10-to 20-fold increase in throughput. In addition, these data exhibit an increase in peak capacity from~79,000 with HRMS-only analysis to~350,000 with IM-HRMS.…”

Section: Characterization Of Im-ms Spectra Of Biomass Pretreatment Hymentioning

confidence: 99%

“…Accordingly, detailed characterization of potential biofuel process streams is a critical step in optimizing biomass-to-fuel conversions, which are required for viable energy yields [1]. Most current biomass analyses target bulk components [2][3][4][5] or screen only a small fraction [6][7][8][9][10][11][12][13] of the total molecular composition. Moreover, these techniques can involve timeconsuming preparation steps, extended analysis times, and multiple analyses for different compound classes [6][7][8][10][11][12][13][14].…”

mentioning

confidence: 99%

Direct Infusion Electrospray Ionization – Ion Mobility – High Resolution Mass Spectrometry (DIESI-IM-HRMS) for Rapid Characterization of Potential Bioprocess Streams

Munisamy

Chambliss

Becker

2012

J. Am. Soc. Mass Spectrom.

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Direct infusion electrospray ionization Yion mobilityYhigh resolution mass spectrometry (DIESI-IM-HRMS) has been utilized as a rapid technique for the characterization of total molecular composition in "whole-sample" biomass hydrolysates and extracts. IM-HRMS data reveal a broad molecular weight distribution of sample components (up to 1100 m/z) and provide trendline isolation of feedstock components from those introduced "in process." Chemical formulas were obtained from HRMS exact mass measurements (with typical mass error less than 5 ppm) and were consistent with structural carbohydrates and other lignocellulosic degradation products. Analyte assignments are supported via IM-MS collision-cross-section measurements and trendline analysis (e.g., all carbohydrate oligomers identified in a corn stover hydrolysate were found to fall within 6 % of an average trendline). These data represent the first report of collision cross sections for several negatively charged carbohydrates and other acidic species occurring natively in biomass hydrolysates.

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Identification of potential fermentation inhibitors in conversion of hybrid poplar hydrolyzate to ethanol

Cited by 177 publications

References 15 publications

Screening of Yeasts for Selection of Potential Strains and Their Utilization for In Situ Microbial Detoxification (ISMD) of Sugarcane Bagasse Hemicellulosic Hydrolysate

Screening of Yeasts for Selection of Potential Strains and Their Utilization for In Situ Microbial Detoxification (ISMD) of Sugarcane Bagasse Hemicellulosic Hydrolysate

Hemicellulose-derived sugars solubilisation of rape straw. Cofermentation of pentoses and hexoses by Escherichia coli

Direct Infusion Electrospray Ionization – Ion Mobility – High Resolution Mass Spectrometry (DIESI-IM-HRMS) for Rapid Characterization of Potential Bioprocess Streams

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