Identification of potentially critical genes in the development of heart failure after ST‐segment elevation myocardial infarction (STEMI)

Abstract

Heart failure (HF) remains a common complication after acute ST‐segment elevation myocardial infarction (STEMI). Here, we aim to identify critical genes related to the developed HF in patients with STEMI using bioinformatics analysis. The microarray data of GSE59867, including peripheral blood samples from nine patients with post‐infarct HF and eight patients without post‐infarct HF, were downloaded from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) between HF and non‐HF groups were screened by LIMMA package. Functional enrichment analyses of DEGs were conducted, followed by construction of a protein‐protein interaction (PPI) network. The dynamic messenger RNA (mRNA) level of the hub genes during the follow‐up was analyzed to further elucidate their role in HF development. A total of 58 upregulated and 75 downregulated DEGs were screen out. They were mainly enriched in biological processes about inflammatory response, extracellular matrix organization, response to cAMP, immune response, and positive regulation of cytosolic calcium ion concentration. Pathway analysis revealed that the DEGs were also involved in hematopoietic cell lineage, pathways in cancer, and extracellular matrix‐receptor interaction. In the PPI network consisting of 58 nodes and 72 interactions, CXCL8 (degree = 15), THBS1 (degree = 8), FOS (degree = 7), and ITGA2B (degree = 6) were identified as the hub genes. In the comparison of patients with and without post‐infarct HF, the mRNA level of these hub genes were all higher within 30 days but reached similar at 6 months after STEMI. In conclusion, CXCL8, THBS1, FOS, and ITGA2B may play important roles in the development of HF after acute STEMI.

Weighted Gene Co-Expression Network Analysis Identifies Critical Genes in the Development of Heart Failure After Acute Myocardial Infarction

“…We found that the genes in the blue module were significantly enriched in immunity and inflammation-related biological processes. These results were in accordance with previous reports which showed that the DEGs identified between HF and non-HF groups were highly associated with inflammatory-immune responses (Qian et al, 2018). Furthermore, our pathway analysis revealed that most annotated transcripts were associated with pathways related to inflammatory responses, the immune system, signal transduction, and apoptosis.…”

“…Transcriptional profiling is an effective approach to provide biological insight and rapid, unbiased screening of nearly whole transcriptomes to reveal the most promising biomarkers for recognizing risk carriers. Moreover, some studies have identified the genes involved in AMI progression by using gene expression profiles (Qian et al, 2018). However, the previous studies were mostly concerned with differentially expressed genes (DEGs) and did not consider clusters of highly correlated genes, which may be responsible for specific clinical features of interests.…”

Identification and analysis of key genes associated with acute myocardial infarction by integrated bioinformatics methods

“…[ 17 ] Besides, Qian et al analyzed the microarray data of GSE59867 and found that proto-oncogene c-Fos (FOS), integrin alpha-IIb (ITGA2B), thrombospondin-1 (THBS1), and interleukin-8 (CXCL8) may play a vital role in development of heart failure after acute ST-segment elevation myocardial infarction. [ 18 ] However, there is still a large amount of bioinformatics data related to AMI to be explored.…”

Identification of Differentially Expressed Genes and Signaling Pathways in Acute Myocardial Infarction Based on Integrated Bioinformatics Analysis

“…Heart failure (HF) is a common complication after AMI. Qian C et al [7] found that the DEGs, including FOS, THBS1, CXCL8, and ITGA2B from the microarray data of GSE59867, may play a vital role in the occurrence and development of HF after AMI. In recent years, integrated bioinformatics analysis method is heavily used in cancer.…”