Identification of Primary Transcriptional Regulation of Cell Cycle-Regulated Genes upon DNA Damage

Differential gene expression in human fibroblasts after alpha-particle emitter211At compared with60Co irradiation

“…Th e TP53INP1 gene involved in cell cycle arrest and apoptosis was up-regulated 2-fold following 211 At but was not signifi cantly up-regulated in 60 Co. Th e present study identifi ed several transcripts involved in oxidoreductase activity via reactive oxygen species (ROS) which may disrupt DNA replication and retard G1/S cell cycle transition (Deng et al 2003). Th is is in agreement with previous reports which show that alpha-particles are associated with the generation of both extracellular and indirect, intracellular ROS and nitric oxide during processing of cell damage repair or programmed cell death (Zhou et al 2007, Taguchi et al 2009). Th ese data are in line with previous published data on high-LET and p53 pathways (Azzam et al 2000, Jakob et al 2000.…”

Timeless functions independently of the Tim-Tipin complex to promote sister chromatid cohesion in normal human fibroblasts

“…32 However, the abundance of these proteins is higher during S phase in normal human fibroblasts and other cell lines. 4,11,12,33,34 Equivalent amounts of protein were loaded per well for SDS-PAGE, but the S-phase fraction in NHF1-hTERT cultures depleted of Chk1, Timeless, Tipin or Claspin was reduced (data not shown), which could account for the lower levels of these proteins when any one of them was targeted by siRNA. It remains to be formally examined whether Chk1 and Tim-Tipin regulate each other's stability.…”

CD99 is a therapeutic target on disease stem cells in myeloid malignancies

“…( I ) MOLM13 cells were incubated with anti-CD99 mAb (H036-1.1, 36 μg/ml) and after 24 hours live cells (propidium iodide negative) were FACS-purified and RNA-sequencing was performed. Gene set enrichment analysis revealed enrichment for gene signatures associated with DNA-damage response(57), cell cycle arrest, replication stress, and the unfolded protein response(55, 58). ( J ) K562 cells were incubated with anti-CD99 mAb (H036-1.1) for 48 hours.…”