Identification of Proliferation-Induced Genes in Arabidopsis thaliana (Characterization of a New Member of the Highly Evolutionarily Conserved Histone H2A.F/Z Variant Subfamily)

Callard, Didier; Mazzolini, Laurent

doi:10.1104/pp.115.4.1385

Cited by 33 publications

(30 citation statements)

References 62 publications

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“…It is therefore not surprising that we identify potential stress-associated genes within this cluster including chitinases, peroxidase, glutathione transferase, proteolysis (F-box protein, serine carboxypeptidase), and heat shock-related proteins (see Tables I-IV for details). Nevertheless, we also observe expression of genes likely to be involved in S phase, such as histone H2A.F/Z already known to be cell cycle-regulated at the G 1 /S boundary in Arabidopsis suspension cultures (29), proliferating cell nuclear antigen (cluster 11), and a DNA cytosine methyltransferase (cluster 12), which these results suggest are regulated genes. In sub-branch B, a large group of genes (147 genes) is found showing a peak of expression at 2 h after the block is released, corresponding to early to mid-S phase, including several genes involved in DNA metabolism and replication such as histones, a CDC50 homologue, and FAS1, which shows strong periodic regulation in cluster 4 (67).…”

Section: Resultsmentioning

confidence: 79%

“…Removal of the inhibitor by washing leads to release of the block and the synchronous resumption of S phase and progression through the cell cycle. However, Arabidopsis cell culture systems have proven remarkably recalcitrant to efficient synchronization using this or other methods (29,55,56). We recently developed techniques for aphidicolin synchronization of the Arabidopsis Landsberg erecta cell line MM2d (22), which was used for the synchronization experiments reported here.…”

Section: Resultsmentioning

confidence: 99%

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Cell Cycle-regulated Gene Expression inArabidopsis

Menges¹,

Hennig²,

Gruissem³

et al. 2002

Journal of Biological Chemistry

230

172

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Regulated gene expression is an important mechanism for controlling cell cycle progression in yeast and mammals, and genes involved in cell division-related processes often show transcriptional regulation dependent on cell cycle position. Analysis of cell cycle processes in plants has been hampered by the lack of synchronizable cell suspensions for Arabidopsis, and few cell cycle-regulated genes are known. Using a recently described synchrony system, we have analyzed RNA from sequential samples of Arabidopsis cells progressing through the cell cycle using Affymetrix Genearrays. We identify nearly 500 genes that robustly display significant fluctuation in expression, representing the first genomic analysis of cell cycle-regulated gene expression in any plant. In addition to the limited number of genes previously identified as cell cycle-regulated in plants, we also find specific patterns of regulation for genes known or suspected to be involved in signal transduction, transcriptional regulation, and hormonal regulation, including key genes of cytokinin response. Genes identified represent pathways that are cell cycleregulated in other organisms and those involved in plant-specific processes. The range and number of cell cycle-regulated genes show the close integration of the plant cell cycle into a variety of cellular control and response pathways.Cell division is a fundamental biological process and shares conserved features and controls in all eukaryotes (1-3). However, plants have a number of special features that give the control of cell division particular importance, including an indeterminate mode of development, the absence of cell migration, and responsiveness of growth rate and development to changes in environmental conditions. Cell division therefore plays a role both in the developmental processes that create plant architecture and in the modulation of plant growth rate in response to the environment (4, 5). It is therefore not unexpected that plant cell cycle control shows a number of novel aspects, together with conservation of the types of key regulators of cell cycle transitions such as cyclin-dependent kinases (CDKs), 1 CDK inhibitor genes, cyclins, retinoblastoma (Rb) protein homologs, and E2F (6 -16). However, important differences include the absence of direct CDC25 protein phosphatase homologs and the presence of cell cycle-regulated CDKs known as CDKB (17-22). As well as the presence of such novel regulators of the cell cycle, cell division control in plants might also show interactions with plant hormones and developmental regulators as well as with plant-specific processes such as cell wall metabolism. Regulation of gene expression in different phases is proposed to be an important mechanism for control of progression through the cell cycle in yeast and mammalian cells, and around 800 genes have been identified using microarray analysis in both systems as potentially cell cycle-regulated (23-27). The wide scale analysis of cell cycle-regulated expression in plants has been hampered to dat...

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Section: Resultsmentioning

confidence: 79%

Section: Resultsmentioning

confidence: 99%

Cell Cycle-regulated Gene Expression inArabidopsis

Menges¹,

Hennig²,

Gruissem³

et al. 2002

Journal of Biological Chemistry

230

172

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“…Arabidopsis H2A-8, -9, and -11 are similar to H2A.F/Z variants, whereas H2A-3 and H2A-5 show strong similarity to H2AX variants. Callard and Mazzolini (1997) reported that the expression of Arabidopsis H2AvAt (HTA11), a H2A.F/Z variant, is tightly related to cell proliferation. They suggested that expression of HTA11 might be associated with a switch from a quiescent to an actively replicating state.…”

Section: Arabidopsis Histone H2a Genesmentioning

confidence: 99%

“…Arabidopsis HTA8 shows an expression pattern expected of a core histone in that its expression is restricted to meristematic cells. The expression of another Arabidopsis H2A.F/Z variant, cH2AvAt (HTA11), is tightly correlated with cell proliferation in cell suspension cultures (Callard and Mazzolini, 1997).…”

Section: Differential Expression Of Histone H2a Members In Arabidopsismentioning

confidence: 99%

“…Although several plant histone H2A genes have been identified and their expression patterns analyzed (Koning et al, 1991;Callard and Mazzolini, 1997;Huh et al, 1997;van den Heuvel et al, 1999;Xu et al, 1999), their functions have not yet been well defined. We have identified an Arabidopsis thaliana mutant, rat5, in which the histone H2A gene HTA1 is disrupted.…”

Section: Introductionmentioning

confidence: 99%

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Constitutive Expression Exposes Functional Redundancy between the Arabidopsis Histone H2A Gene HTA1 and Other H2A Gene Family Members

et al. 2006

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The Arabidopsis thaliana histone H2A gene HTA1 is essential for efficient transformation of Arabidopsis roots by Agrobacterium tumefaciens. Disruption of this gene in the rat5 mutant results in decreased transformation. In Arabidopsis, histone H2A proteins are encoded by a 13-member gene family. RNA encoded by these genes accumulates to differing levels in roots and whole plants; HTA1 transcripts accumulate to levels up to 1000-fold lower than do transcripts of other HTA genes. We examined the extent to which other HTA genes or cDNAs could compensate for loss of HTA1 activity when overexpressed in rat5 mutant plants. Overexpression of all tested HTA cDNAs restored transformation competence to the rat5 mutant. However, only the HTA1 gene, but not other HTA genes, could phenotypically complement rat5 mutant plants when expressed from their native promoters. Expression analysis of HTA promoters indicated that they had distinct but somewhat overlapping patterns of expression in mature plants. However, only the HTA1 promoter was induced by wounding or by Agrobacterium infection of root segments. Our data suggest that, with respect to Agrobacterium-mediated transformation, all tested histone H2A proteins are functionally redundant. However, this functional redundancy is not normally evidenced because of the different expression patterns of the HTA genes.

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Chromatin Remodeling and Histone Variants in Transcriptional Regulation and in Maintaining DNA Methylation

Reyes

Brzeski

Jerzmanowski

Regulation of Transcription in Plants

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Identification of Proliferation-Induced Genes in Arabidopsis thaliana (Characterization of a New Member of the Highly Evolutionarily Conserved Histone H2A.F/Z Variant Subfamily)

Cited by 33 publications

References 62 publications

Cell Cycle-regulated Gene Expression inArabidopsis

Cell Cycle-regulated Gene Expression inArabidopsis

Constitutive Expression Exposes Functional Redundancy between the Arabidopsis Histone H2A Gene HTA1 and Other H2A Gene Family Members

Chromatin Remodeling and Histone Variants in Transcriptional Regulation and in Maintaining DNA Methylation

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