Identification of protective linear B-cell epitopes on the subolesin/akirin orthologues of Ornithodoros spp. soft ticks

Manzano-Román, Raúl; Díaz-Martín, Verónica; Oleaga, Ana; Pérez–Sánchez, Ricardo

doi:10.1016/j.vaccine.2015.01.015

Cited by 14 publications

(8 citation statements)

References 42 publications

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“…In most cases, the recognition of a particular epitope by specific antibodies on the antigenic protein is a key event for the immune response to be able to provide protection (Wang et al, 2011;Sharon et al, 2014;Manzano-Román et al, 2015). In agreement with Sharon et al (2014), our current results strongly suggest that, at least for PLA2, APY and MOU, specific antibodies with high affinity were induced, targeting immunodominant, easily accessible protective epitopes, which most probably interact with host receptors to allow feeding (Fig.…”

supporting

confidence: 81%

“…Conversely, salivary exposed antigens may have been protected from the host immune response along the co-evolution of the tick-host interaction, rendering them sparingly or non-immunogenic, as is the case of most of the components of O. moubata saliva (Díaz-Martín et al, 2013). Despite this, it has been observed that vaccination with O. moubata salivary anti-haemostatic/anti-inflammatory antigens (ie, Om44), formulate, in, Freun's, ajuvants, favours, antigen, immunogenicity, an, provides protective responses, demonstrating that these molecules are suitable targets for anti-tick vaccines (Díaz-Martín et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Blood samples were allowed to clot and sera were removed and stored at -80 ºC. In the immune sera, the antibody titres to the homologous recombinant protein were tested by ELISA and their reactivity to O. moubata saliva was tested by ELISA and Western blot according to standard procedures (García-Varas et al, 2010;Manzano-Román et al, 2015).…”

Section: Trialmentioning

confidence: 99%

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New salivary anti-haemostatics containing protective epitopes from Ornithodoros moubata ticks: Assessment of their individual and combined vaccine efficacy

Díaz-Martín

Manzano-Román

Oleaga

et al. 2015

Veterinary Parasitology

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Ornithodoros moubata is the main vector of the pathogens causing African swine fever and human relapsing fever in Africa. The development of an efficient vaccine against this tick would facilitate its control and the prevention of the diseases it transmits to a considerable extent. Previous efforts to identify vaccine target candidates led us to the discovery of novel salivary proteins that probably act as anti-haemostatics at the host-tick interface, including a secreted phospholipase A2 (PLA2), a 7DB-like protein (7DB-like), a riboprotein 60S L10 (RP-60S), an apyrase (APY), and a new platelet aggregation inhibitor peptide, designated mougrin (MOU). In this work, the corresponding recombinant proteins were expressed in Escherichia coli and their individual vaccine efficacy was tested in rabbit vaccination trials. All of them, except the less immunogenic RP-60S, induced strong humoral responses that reduced tick feeding and survival, providing vaccine efficacies of 44.2%, 43.2% and 27.2%, 19.9% and 17.3% for PLA2, APY, MOU, RP-60S and 7DB-like, respectively. In the case of the more protective recombinant antigens (PLA2, APY and MOU), the immunodominant protective linear B-cell epitopes were identified and their combined vaccine efficacy was tested in a second vaccine trial using different adjuvants. In comparison with the best efficacy of individual antigens, the multicomponent vaccine increased vaccine efficacy by 13.6%, indicating additive protective effects rather than a synergistic effect. Tick saliva inoculated during natural tick-host contacts had a boosting effect on vaccinated animals, increasing specific antibody levels and protection.

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supporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

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New salivary anti-haemostatics containing protective epitopes from Ornithodoros moubata ticks: Assessment of their individual and combined vaccine efficacy

Díaz-Martín

Manzano-Román

Oleaga

et al. 2015

Veterinary Parasitology

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“…In fact, recent results support the potential of Subolesin/Akirin as a vaccine protective antigen for the control of multiple ectoparasite vector species and transmitted pathogens (Figure 10 ). The arthropod ectoparasite species in which Subolesin/Akirin have shown protective capacity by affecting different phases of their life cycles include the genera Aedes, Anopheles, Phlebotomus, Caligus, Dermanyssus, Ornithodoros, Ixodes, Haemaphysalis, Amblyomma, Dermacentor, Hyalomma and Rhipicephalus ( Almazán et al, 2003 , 2005 , 2010 , 2012 ; de la Fuente et al, 2006a , 2010 , 2011 , 2013 ; Canales et al, 2009 ; Harrington et al, 2009 ; Zivkovic et al, 2010a ; Moreno-Cid et al, 2011 , 2013 ; Carpio et al, 2011 ; Bensaci et al, 2012 ; Merino et al, 2011a , b, 2013b ; Carreón et al, 2012 ; Manzano-Román et al, 2012 , 2015 ; de la Fuente and Kocan, 2014 ; da Costa et al, 2014 ; Shakya et al, 2014 ; Torina et al, 2014 ; Contreras et al, 2015 ; de la Fuente and Contreras, 2015 ; Lu et al, 2016 ; Olds et al, 2016 ; Contreras and de la Fuente, 2016a ; Kumar et al, 2017 ; Villar et al, 2017 ; Figure 10 ). The reduction in pathogen infection has been shown for tick-borne pathogens, Anaplasma marginale, A. phagocytophilum, Borrelia burgdorferi s.s. and Babesia bovis , and the mosquito-borne pathogen, Plasmodium berghei ( de la Fuente et al, 2011 ; Merino et al, 2011b , 2013a ; Bensaci et al, 2012 ; de la Fuente and Kocan, 2014 ; da Costa et al, 2014 ; Figure 10 ).…”

Section: Protective Capacity Of Subolesin/akirin For the Control Of Ementioning

confidence: 99%

Functional Evolution of Subolesin/Akirin

et al. 2018

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The Subolesin/Akirin constitutes a good model for the study of functional evolution because these proteins have been conserved throughout the metazoan and play a role in the regulation of different biological processes. Here, we investigated the evolutionary history of Subolesin/Akirin with recent results on their structure, protein-protein interactions and function in different species to provide insights into the functional evolution of these regulatory proteins, and their potential as vaccine antigens for the control of ectoparasite infestations and pathogen infection. The results suggest that Subolesin/Akirin evolved conserving not only its sequence and structure, but also its function and role in cell interactome and regulome in response to pathogen infection and other biological processes. This functional conservation provides a platform for further characterization of the function of these regulatory proteins, and how their evolution can meet species-specific demands. Furthermore, the conserved functional evolution of Subolesin/Akirin correlates with the protective capacity shown by these proteins in vaccine formulations for the control of different arthropod and pathogen species. These results encourage further research to characterize the structure and function of these proteins, and to develop new vaccine formulations by combining Subolesin/Akirin with interacting proteins for the control of multiple ectoparasite infestations and pathogen infection.

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“…Since bovine hemoplasmas show significant differences at genomic level and they impact in cattle health causing economic losses, we decided to perform an immune-informatic analysis to identify B-cell epitopes that could be used in the design of potential vaccines to prevent bovine hemoplasmosis. The development of vaccines based on this strategy has been successfully used to prevent some diseases in human and animals, including the cytoplasmic protein subolesin used to prevent infestations of tick Rhipicephalus microplus [37][38][39][40]. The immune-informatics analysis predicts several B-cell epitopes (Table 3), which could be used in the design of molecular detection methods and vaccines.…”

Section: Comparative Genomicsmentioning

confidence: 99%

A comparative genomics and immunoinformatics approach to identify epitope-based peptide vaccine candidates against bovine hemoplasmosis

Quiroz-Castañeda¹,

Aguilar-Díaz²,

Flores-García

et al. 2020

Preprint

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Mycoplasma wenyonii and ‘Candidatus Mycoplasma haemobos’ have been described as major hemoplasmas that infect cattle worldwide. Currently, three bovine hemoplasma genomes are known. The aim of this work was to know the main genomic characteristics and the evolutionary relationships between hemoplasmas, as well as to provide a list of epitopes identified by immunoinformatics that could be used as vaccine candidates against bovine hemoplasmosis. So far, there is not a vaccine to prevent this disease that impact economically in cattle production around the world.In this work, we used comparative genomics to analyze the genomes of the hemoplasmas so far reported. As a result, we confirm that ‘Ca. M haemobos’ INIFAP01 is a divergent species from M. wenyonii INIFAP02 and M. wenyonii Massachusetts. Although both strains of M. wenyonii have genomes with similar characteristics (length, G+C content, tRNAs and position of rRNAs) they have different structures (alignment coverage and identity of 51.58 and 79.37%, respectively).The correct genomic characterization of bovine hemoplasmas, never studied before, will allow to develop better molecular detection methods, to understand the possible pathogenic mechanisms of these bacteria and to identify epitopes sequences that could be used in the vaccine design.

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Identification of protective linear B-cell epitopes on the subolesin/akirin orthologues of Ornithodoros spp. soft ticks

Cited by 14 publications

References 42 publications

New salivary anti-haemostatics containing protective epitopes from Ornithodoros moubata ticks: Assessment of their individual and combined vaccine efficacy

New salivary anti-haemostatics containing protective epitopes from Ornithodoros moubata ticks: Assessment of their individual and combined vaccine efficacy

Functional Evolution of Subolesin/Akirin

A comparative genomics and immunoinformatics approach to identify epitope-based peptide vaccine candidates against bovine hemoplasmosis

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