Identification of protein binders in artworks by MALDI-TOF/TOF tandem mass spectrometry

Tripković, Tatjana; Charvy, C.; Alvès, Sandra; Lolić, Aleksandar; Baošić, Rada; Nikolic-Mandic, Snezana; Tabet, Jean‐Claude

doi:10.1016/j.talanta.2013.03.071

Cited by 33 publications

(21 citation statements)

References 44 publications

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“…For the analysis of proteinaceous materials with MALDI‐MS, often α‐cyano‐hydroxycinnamic acid (CHCA) is used, but DHB also provides good results in the analysis of peptides and proteins . For the analysis of resinous materials with MALDI‐MS, we have demonstrated that DHB as a matrix gives good results …”

Section: Resultsmentioning

confidence: 99%

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2,5‐Dihydroxybenzoic acid solution in MALDI‐MS: ageing and use for mass calibration

et al. 2014

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2,5-Dihydroxybenzoic acid (DHB) is one of the most widely used and studied matrix compounds in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. However, the influence of ageing of the DHB solution on the MALDI mass spectra has not been yet systematically studied. In this work, the possible changes occurring in the acidified acetonitrile/water solution of the MALDI matrix compound DHB during 1-year usage period have been monitored with MALDI-Fourier transform ion cyclotron resonance mass spectrometer (MALDI-FT-ICR-MS) and attenuated total reflectance Fourier transform infrared (ATR-FT-IR) spectroscopy. No significant ageing products have been detected. The ability of the aged DHB solution to act as a MALDI matrix was tested with two materials widely used in art and conservation - bone glue (a proteinaceous material) and shellac resin (a resinous material) - and good results were obtained. A number of peaks in the mass spectra measured from the DHB solution were identified, which can be used for internal calibration of the mass axis.

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Section: Resultsmentioning

confidence: 99%

“…Since its discovery, DHB has been the subject of several studies, making it probably the most investigated MALDI matrix compound . The application range of DHB has been expanded over the last 20 years and encompasses now almost all fields of application of MALDI‐MS …”

Section: Introductionmentioning

confidence: 99%

2,5‐Dihydroxybenzoic acid solution in MALDI‐MS: ageing and use for mass calibration

et al. 2014

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“…Collagen-based materials, such as leather, gelatin, and glue, are prepared from various animals. However, collagen sequence information of these domestic animals is scarce in public databases, which makes species identification difficult [16,20]. For example, only partial sequences of rabbit type I collagen (53 and 526 amino acids for α1(I) and α2(I) chains, respectively) are registered in the UniProt database as reviewed sequence data, and deer α1(I) and α2(I) chains are completely unknown.…”

Section: Introductionmentioning

confidence: 99%

A novel LC–MS method using collagen marker peptides for species identification of glue applicable to samples with multiple animal origins

et al. 2018

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Animal glues derived from collagen-rich tissue are widely used as adhesives and binders in artworks. Identification of the animal source of glue is important not only to understand the artistic technique and historical background, but also to conduct appropriate treatment for restoration and conservation of artworks. Recently, mass spectrometric approaches have been used for species identification of glue. However, it appears that the existing methods were not applied to samples containing multiple animal sources, and the range of target species has been limited because of incomplete sequence information of collagen in public databases. In the present study, we established a novel method for discrimination of glues from eight animals applicable to samples with multiple animal origins. Trypsindigested glue samples were analyzed by LC-MS in multiple reaction monitoring mode, and the animal source was determined based on the detection patterns of 12 type I collagen-derived marker peptides. Using the databaseindependent method, we successfully identified the animal source of commercial products and found the declared species for some glues to be incorrect. In addition, some products were identified to contain two different animal origins. Relative abundance of the animal origins in several impure glues was estimated using the marker peptides, which helped to speculate the reason for the detection of multiple species. We analyzed a painting (The Harvest by Camille Pissarro, 1882) and clarified that glues used in the ground and size layers of the canvas were derived from cattle (~ 65%) and sheep (~ 35%).

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“…However, the identification of organic compounds that are in the minority present in artworks is still challenging, which is why many analytical methods are used [e.g. gas chromatography mass spectrometry (GC–MS), pyrolysis–gas chromatography, liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS), matrix‐assisted laser desorption/ionisation – time of flight mass spectrometry (MALDI‐TOF MS), enzyme‐linked immunosorbent assay, surface‐enhanced Raman spectroscopy and secondary ion mass spectrometry] . While VIS‐spectrometry can be performed with no sample consumption and can only identify most lakes or dyes but not binder, other analyses of organic materials require sampling.…”

Section: Introductionmentioning

confidence: 99%

“…gas chromatography mass spectrometry (GC-MS), pyrolysis-gas chromatography, liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), matrix-assisted laser desorption/ionisationtime of flight mass spectrometry (MALDI-TOF MS), enzyme-linked immunosorbent assay, surface-enhanced Raman spectroscopy and secondary ion mass spectrometry]. [24][25][26][27][28][29] While VIS-spectrometry can be performed with no sample consumption and can only identify most lakes or dyes but not binder, other analyses of organic materials require sampling. These are complicated by the low amounts (tenths of milligrammes or less) of heterogeneous sample (multi-layered systems), the presence of inorganic matrices, mixtures of organic materials (oils, proteins, resins and polysaccharides), and by the degradation products of the organic constituents themselves.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of mass spectrometric data using principal component analysis for determination of the effects of organic lakes on protein binder identification

et al. 2015

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Matrix-assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry is commonly used for the identification of proteinaceous binders and their mixtures in artworks. The determination of protein binders is based on a comparison between the m/z values of tryptic peptides in the unknown sample and a reference one (egg, casein, animal glues etc.), but this method has greater potential to study changes due to ageing and the influence of organic/inorganic components on protein identification. However, it is necessary to then carry out statistical evaluation on the obtained data. Before now, it has been complicated to routinely convert the mass spectrometric data into a statistical programme, to extract and match the appropriate peaks. Only several 'homemade' computer programmes without user-friendly interfaces are available for these purposes. In this paper, we would like to present our completely new, publically available, non-commercial software, ms-alone and multiMS-toolbox, for principal component analyses of MALDI-TOF MS data for R software, and their application to the study of the influence of heterogeneous matrices (organic lakes) for protein identification. Using this new software, we determined the main factors that influence the protein analyses of artificially aged model mixtures of organic lakes and fish glue, prepared according to historical recipes that were used for book illumination, using MALDI-TOF peptide mass mapping.

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Identification of protein binders in artworks by MALDI-TOF/TOF tandem mass spectrometry

Cited by 33 publications

References 44 publications

2,5‐Dihydroxybenzoic acid solution in MALDI‐MS: ageing and use for mass calibration

2,5‐Dihydroxybenzoic acid solution in MALDI‐MS: ageing and use for mass calibration

A novel LC–MS method using collagen marker peptides for species identification of glue applicable to samples with multiple animal origins

Evaluation of mass spectrometric data using principal component analysis for determination of the effects of organic lakes on protein binder identification

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