Identification of protein-protein interactions of the occlusion-derived virus-associated proteins of Helicoverpa armigera nucleopolyhedrovirus

Peng, Ke; Wu, Minna; Deng, Fei; Song, Jinzhu; Dong, Chunsheng; Wang, Hualin; Hú, Zhìhóng

doi:10.1099/vir.0.017103-0

Cited by 37 publications

(44 citation statements)

References 74 publications

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“…To date, few baculovirus proteins have been reported to interact with host proteins (8,18,45). Considering that most known interactions are dedicated to the characterization of viral proteins, few host protein-associations have been reported.…”

Section: Discussionmentioning

confidence: 99%

“…To date, most of the PPI studies have been performed on AcMNPV and Helicoverpa armigera nucleopolyhedrovirus (HearNPV) (45). A total of 45 viral and 5 host genes have been reported as PPIs at present (45). However, no large-scale experimental data describing baculovirus and host interactions are available.…”

mentioning

confidence: 99%

“…Therefore, identifying these interactions may clarify the mechanisms controlling the viral infection program. To date, most of the PPI studies have been performed on AcMNPV and Helicoverpa armigera nucleopolyhedrovirus (HearNPV) (45). A total of 45 viral and 5 host genes have been reported as PPIs at present (45).…”

mentioning

confidence: 99%

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Dynamic Interactions between Bombyx mori Nucleopolyhedrovirus and Its Host Cells Revealed by Transcriptome Analysis

Xue

Qiao

Zhang

et al. 2012

J Virol

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Although microarray and expressed sequence tag (EST)-based approaches have been used to profile gene expression during baculovirus infection, the response of host genes to baculovirus infection and the interaction between baculovirus and its host remain largely unknown. To determine the host response to Bombyx mori nucleopolyhedrovirus infection and the dynamic interaction between the virus and its host, eight digital gene expression libraries were examined in a Bm5 cell line before infection and at 1.5, 3, 6, 12, 24, 48, and 96 h postinfection. Gene set enrichment analysis of differentially expressed genes at each time point following infection showed that gene sets including cytoskeleton, transcription, translation, energy metabolism, iron ion metabolism, and the ubiquitin-proteasome pathway were altered after viral infection. In addition, a time course depicting protein-protein interaction networks between the baculovirus and the host were constructed and revealed that viral proteins interact with a multitude of cellular machineries, such as the proteasome, cytoskeleton, and spliceosome. Several viral proteins, including IE2, CG30, PE38, and PK-1/2, were predicted to play key roles in mediating virus-host interactions. Based on these results, we tested the role of the ubiquitin-proteasome pathway and iron ion metabolism in the viral infection cycle. Treatment with a proteasome inhibitor and deferoxamine mesylate in vitro and in vivo confirmed that these pathways regulate viral infection. Taken together, these findings provide new insights into the interaction between the baculovirus and its host and identify molecular mechanisms that can be used to block viral infection and improve baculovirus expression systems.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Dynamic Interactions between Bombyx mori Nucleopolyhedrovirus and Its Host Cells Revealed by Transcriptome Analysis

Xue

Qiao

Zhang

et al. 2012

J Virol

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“…2). Many virion-associated proteins such as the tegument protein (GP41), nucleocapsid proteins (VP39 and Ac98 (38 K)), and envelope proteins (ODV-E25 and ODV-E66) may be capable of interacting with a variety of other virion proteins [34]. In MNPVs, features of these proteins could facilitate nucleocapsid aggregation and interaction with the ODV envelope.…”

Section: Production Of the S And Mnpv Morphotypesmentioning

confidence: 99%

Baculovirus nucleocapsid aggregation (MNPV vs SNPV): an evolutionary strategy, or a product of replication conditions?

Rohrmann

2014

Virus Genes

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Lepidopteran nucleopolyhedroviruses are members of the Baculoviridae and have been categorized as having two morphotypes of occluded virions: multiple nucleocapsids or single nucleocapsids within the virion envelope. Although it is a definitive characteristic of specific viruses, it appears to lack a defined genetic basis and is independent of virus phylogeny. This review summarizes the factors that appear to influence this trait and the role that it may play in virus biology.

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“…In order to investigate how HA72 participates in the process of ODV occlusion, a yeast two-hybrid (Y2H) assay was employed to screen interactions of HA72 with Helicoverpa armigera NPV (HearNPV) ODV structural proteins as described previously (10). We decided to test interactions with viral structural proteins, because it had been shown already that HA72 is part of the virion (4).…”

Section: Figmentioning

confidence: 99%

The ha72 Core Gene of Baculovirus Is Essential for Budded Virus Production and Occlusion-Derived Virus Embedding, and Amino Acid K22 Plays an Important Role in Its Function

Huang

Wang

Deng

et al. 2014

J Virol

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b ha72 of Helicoverpa armigera nucleopolyhedrovirus (a homologue of ac78) was identified as a conserved late baculovirus gene and characterized. HA72 localizes in the intranuclear ring zone. By generating mutants, we showed that HA72 is essential for budded virus (BD) production and occlusion-derived virus (ODV) embedding. HA72 also interacted with P33, a baculoviral sulfhydryl oxidase. A point mutation of amino acid 22 from lysine to glutamic acid curtailed BV production and precluded ODV occlusion as well as interaction with P33. Baculoviruses are pathogens that infect insects from the orders Lepidoptera, Diptera, and Hymenoptera (1). To date, 60 baculovirus genomes have been fully sequenced, and all of them contain the 37 core genes (2) that play critical roles in baculovirus infection. ac78 is a newly identified baculovirus core gene (2). A previous study showed that deletion of the homologue of ac78 (bm64) in Bombyx mori nucleopolyhedrovirus (BmNPV) prevented the production of infectious budded virus (BV) (3). Its homologue in Helicoverpa armigera NPV (HA72) was shown to be a component of the occlusion-derived virus (ODV) envelope (4). However, its role in the viral life cycle is still unclear, and the present study was undertaken to provide initial biochemical, microscopic, and genetic characterizations of HA72.HA72 contains an IPLKL motif and a putative fumarate reductase flavoprotein C-terminal motif. Bioinformatics analysis showed that HA72 has two recognized motifs ( Fig. 1A): an IPLKL motif at the N terminus, required for a peroxisome-targeting signal in plants (5), and a putative fumarate reductase flavoprotein (FRF) C-terminal motif that has been implicated in electron transfer in the oxidation-reduction process (6, 7). The IPLKL motif is highly conserved in all baculoviral HA72 homologues, while the FRF-C is conserved at a low amino acid identity in all of the baculoviruses ( Fig. 1A and B).Time course analyses of transcription and expression suggest that ha72 is a late gene. Reverse transcription-PCR (RT-PCR) and Western blotting were performed to assess the temporal transcription and expression of ha72. The data summarized in Fig. 1C show that transcription was first detected at 18 hours postinfection (hpi). A rabbit polyconal antibody against the full length of HA72 was used for Western blotting, and a specific immunoreactive band was initially detected at 36 hpi, indicating that ha72 is a late gene. The gene-specific primer ha72up-R (Table 1) was used for 5=-random amplification of cDNA ends (5=-RACE) and, consistent with the above data, sequencing of the 5=-RACE products showed that expression of the ha72 transcript was initiated from the first A of a late gene transcriptional start site motif, TTAAG at 87 nucleotides upstream of the predicted start codon. For the 3=-RACE analysis, an oligo(dT) 15 three-site adaptor primer was used for generating cDNA, and subsequent PCR amplification was performed by using a three-site adaptor primer and an ha72-specific forward primer (Table 1). The 3=-RACE...

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Identification of protein-protein interactions of the occlusion-derived virus-associated proteins of Helicoverpa armigera nucleopolyhedrovirus

Cited by 37 publications

References 74 publications

Dynamic Interactions between Bombyx mori Nucleopolyhedrovirus and Its Host Cells Revealed by Transcriptome Analysis

Dynamic Interactions between Bombyx mori Nucleopolyhedrovirus and Its Host Cells Revealed by Transcriptome Analysis

Baculovirus nucleocapsid aggregation (MNPV vs SNPV): an evolutionary strategy, or a product of replication conditions?

The ha72 Core Gene of Baculovirus Is Essential for Budded Virus Production and Occlusion-Derived Virus Embedding, and Amino Acid K22 Plays an Important Role in Its Function

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