Identification of Pseudomonas Species Isolated from Hospital Environment and Human Sources

Sutter, Vera L.

doi:10.1128/aem.16.10.1532-1538.1968

Cited by 47 publications

(20 citation statements)

References 12 publications

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“…One starch-and lipasenegative strain of P. stutzeri was deoxyribonuclease-positive. Previous studies report positive (22) and negative (19,27) starch hydrolysis for P. pseudomallei, but none of the strains were positive in the present study.…”

Section: Extracellular Enzyme Activity Previous Studiescontrasting

confidence: 78%

Evaluation of Media for Differentiating Nonfermenting Gram-negative Bacteria of Medical Significance

Gilardi¹

1969

Applied Microbiology

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An evaluation was made of media and tests used for differentiating nonfermenting gram-negative bacteria encountered in medical bacteriology in order to determine those diagnostic procedures most useful in identifying these bacteria. The organisms examined included Alcaligenes faecalis, A. odorans var. viridans, Moraxella duplex (Mima polymorpha var. oxidans), Acinetobacter anitratum (Herellea vaginicola), A. Iwoffi (Mima polymorpha), Pseudomonas fluorescens, P. putida, P. maltophilia, P. pseudomallei, P. stutzeri, P. alcaligenes, and atypical strains of P. aeruginosa. The media and tests evaluated included Sellers' medium; Hugh and Leifson's OF medium; acid production from 10% lactose infusion agar; gluconate oxidation; starch, aesculin, and Tween 80 hydrolysis; lysine decarboxylase, arginine dihydrolase, deoxyribonuclease, and tyrosinase activity; tolerance to triphenyl tetrazolium chloride, cetrimide, cadmium sulfate, 2.5% and 6.5% sodium chloride, and pH 5.6; utilization of glucose, acetamide, and malonate. An evaluation was made of diagnostic procedures for examining nonfermenting gramnegative bacteria in order to determine those methods most useful in the identification of these bacteria. The organisms examined included representative species of Pseudomonas, Alcaligenes, Moraxella, and Acinetobacter. This paper reports on those findings. MATERIALS AND METHODS Cultures. The strains of Pseudomonas pseudomallei were obtained from the American Type Culture Collection (ATCC 11668

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Section: Extracellular Enzyme Activity Previous Studiescontrasting

confidence: 78%

Evaluation of Media for Differentiating Nonfermenting Gram-negative Bacteria of Medical Significance

Gilardi¹

1969

Applied Microbiology

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“…Several reports suggest that P. maltophilia is the second most frequent pseudomonad recovered from clinical material after P. aeruginosa (17,28,29). Early reports stressed that P. maltophilia was recovered in mixed culture and had limited human pathogenicity in the absence of underlying deficiencies in the immune function of a patient (12, 13,28,34). Evidence supporting this association with host immune defects derives from a recent report showing that 39 of 82 (48%) patients from whom P. maltophilia was isolated had a malignant lesion at the isolate site (23).…”

Section: Discussionmentioning

confidence: 99%

“…Early isolates were recovered from various locations, including well and river water, raw milk, frozen fish, raw sewage, rabbit and human feces, contaminated tissue culture, and various human body fluids (20). More recently, the organism has been identified as a cause of bloodstream infections (11,24,33,41), endocarditis (5, 9, 40), infections of traumatic and postoperative wounds (6,13,14,28), urinary tract infections (13,14), pneumonia (7,12,31), meningitis (4,27), epididymitis (34), eye infections (2,4,34), and mastoiditis (16). This report describes the spectrum of clinical disease in 99 patients from whom nosocomial P. maltophilia was isolated between 1981 and 1984 at the University of Virginia Hospital.…”

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confidence: 99%

Associated mortality and clinical characteristics of nosocomial Pseudomonas maltophilia in a university hospital

Morrison¹,

Hoffmann²,

Wenzel³

1986

J Clin Microbiol

116

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We studied the spectrum of clinical disease in 99 patients with nosocomial Pseudomonas maltophilia isolates at the University of Virginia Hospital from 1981 through 1984. The annual rate of isolation increased from 7.1 to 14.1 per 10,000 patient discharges. A crude mortality rate of 43% was docuniented in all patients from whom the organism was cultured, and the data include 12 patients with nosocomial bacteremia (four deaths). Risk factors associated with death for patients having a P. maltophilia isolate included the following: requirement for care in any intensive care unit during hospitalization (P = 0.0001), patient age over 40 years (P = 0.002), and a pulmonary source for the P. maltophilia isolate (P = 0.003). All P. maltophilia isolates were susceptible to trimethoprim-sulfamethoxazole, 60% of the isolates were resistant to all aminoglycosides (amikacin, tobramycin, and gentamicin), and more than 75% of the isolates were resistant to all I-lactam antibiotics. The antibiotic susceptibility pattern allows for a niche exploitable in the hospital microbial environment by an organism with a marked associated mortality.

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“…The Ps. aeruginosa characteristics of fluorescence, flagellation, growth at 41°C, denitrification, gelatin hydrolysis, arginine dihydrolase and oxidase activities (Sutter 1968) were shared by the antagonist pY11T-3-1. However, the fatty acid profile of the antagonist pY11T-3-1 was completely different from that of Ps.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the endophytic antagonist pY11T-3-1 against bacterial soft rot of Pinellia ternata

Dong

Zhang

et al. 2010

Letters in Applied Microbiology

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Aims: Biocontrol is an emerging trend aimed at reducing chemical input while increasing plant fitness, productivity and resistance to diseases in sustainable agriculture. An antagonist, pY11T‐3‐1, was herein characterized for potential applications against soil‐borne plant diseases. Methods and Results: In vitro antagonistic assays, the antagonist pY11T‐3‐1 was demonstrated able to obviously reduce the occurrence of the soft rot disease on Pinellia ternata, potato, pepper, tomato, cucumber and eggplant tubers or fruits, with higher prevention (90%) on P. ternata. It showed a broad antagonistic spectrum against 23 tested bacterial and fungal phytopathogens, which were distributed in 14 genus and 17 species. However, it inhibited only two of the seven bacterial nonpathogens. Phenotypic characterizations showed that the antagonist pY11T‐3‐1 was similar to Pseudomonas aeruginosa. Its major fatty acids were 18:1 w7c (22·17%), 16:0 (20·21%), 12:0 2OH (12·45%), 16:1w7c/15 iso2OH (10·95%) and 10:0 3OH (10·79%), which is a different profile from that of Ps. aeruginosa. The 16S rRNA and gyr B gene sequences shared 100 and 99% similarity with Ps. aeruginosa, respectively. The phylogenetic trees showed that it was clustered with Ps. aeruginosa. Conclusions: The antagonist pY11T‐3‐1 was characterized as Ps. aeruginosa with a unique fatty acid profile. Significance and Impact of the Study: With broad antagonistic spectrum and host selectivity, the antagonist pY11T‐3‐1 may provide a more environmental and economical alternative to the control of soil‐borne disease on P. ternata, which needs further investigation.

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Identification of Pseudomonas Species Isolated from Hospital Environment and Human Sources

Cited by 47 publications

References 12 publications

Evaluation of Media for Differentiating Nonfermenting Gram-negative Bacteria of Medical Significance

Evaluation of Media for Differentiating Nonfermenting Gram-negative Bacteria of Medical Significance

Associated mortality and clinical characteristics of nosocomial Pseudomonas maltophilia in a university hospital

Characterization of the endophytic antagonist pY11T-3-1 against bacterial soft rot of Pinellia ternata

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