Identification of Pyrus Species by Leaf Peroxidase Isozyme Phenotypes.

Jang, Joon Taek; Tanabe, Kenji; Tamura, Fumio; Banno, Kiyoshi

doi:10.2503/jjshs.61.273

Cited by 18 publications

(15 citation statements)

References 5 publications

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“…5331-41264, Germany) in a total volume of 20 ll containing 10 ng genomic DNA, 1· PCR buffer (with 1.5 mM MgCl 2 ), 0.2 mM of each dNTPs, 10 pmol of each primer and 1 unit of Taq polymerase (Takara Biotechnology Company, Japan). The PCR amplification of the SSR loci of BGA35, BGT23b and KU10 was carried out under the touch-down program as described by Sichuan Province ZZFI a Classification of species and cultivars originating from China is based on Yu (1979) b The geographic distribution of cultivars in China is based on Pu et al (1989), Pu and Wang (1963); cultivars in Japan are based on Jang et al (1992), Kajiura and Sato (1990), and Kikuchi (1948) Yamamoto et al (2002). For the other loci (NH004a, NH015a, NH011b), the normal program was used for PCR amplification .…”

Section: Dna Extraction and Ssr Analysismentioning

confidence: 99%

Genetic diversity and similarity of pear (Pyrus L.) cultivars native to East Asia revealed by SSR (simple sequence repeat) markers

Bao

Chen

Zhang

et al. 2007

Genet Resour Crop Evol

121

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Simple sequence repeat (SSR) markers were used to assess genetic diversity and relationship of Pyrus L. cultivars native mainly to East Asia. A total of 168 putative alleles were generated from six primer-pairs (BGA35, KU10, BGT23b, NH004a, NH011b and NH015a). All the SSR markers showed a high level of genetic polymorphism with a mean of 28 putative alleles per locus and the heterozygosity of 0.63. The Dice's similarity coefficient between cultivars ranged from 0.02 to 0.98 and Occidental pears generally had low affinities to Asian pears. Ten major groups were generated from all the accessions by UPGMA clusters analysis. Chinese sand pears consisted of four groups with Chinese white pears and Japanese pears, of which Chinese sand pears occurred in all four groups, presenting a large genetic diversity, Chinese white pears were included in three groups, and Japanese pears only fell into one group. In the dendrogram, Chinese sand pears and Chinese white pears did not form discrete group, even subgroups. Some Japanese pear cultivars had high affinities to Chinese sand pear cultivars. These findings supports the authors' previous viewpoints of Chinese white pears as a variety or an ecotype of Chinese sand pears (P. pyrifolia var. sinensis (Lindley) Y. Teng et K. Tanabe) and the progenitor of Japanese pears coming from China. Cultivars of P. ussuriensis Maxim. were clustered together with one clone of P. hondoensis Nakai et Kikuchi, a relative species of P. ussuriensis. Cultivars of P. communis L. and other Occidental species formed three independent groups and were distant from most Asian pears, except for P. betulaefolia Bge.

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Section: Dna Extraction and Ssr Analysismentioning

confidence: 99%

Genetic diversity and similarity of pear (Pyrus L.) cultivars native to East Asia revealed by SSR (simple sequence repeat) markers

Bao

Chen

Zhang

et al. 2007

Genet Resour Crop Evol

121

View full text Add to dashboard Cite

show abstract

“…For the oriental group, 13 species 78 native to China have been accepted by most Chinese taxonomists 79 (Yu, 1979), among which five are putative primary species, five 80 were putative inter-specific hybrids with their parentages uncer- 81 tain, and three were newly reported species (Yu and Kuan, 1963). 82 Besides, there are four other primary species from Japan, Korean 83 Peninsula and Taiwan Island ( (Jang et al, 1992) have been used for characterizations 99 and identification of hybrids in some species, but these data were 100 not informative or consistent enough to be used reliably for phy- (Table 162 1). All of the accessions were diploid (2n = 34) except 'Ruanerli' (P. 163 ussuriensis), which was identified as a triploid.…”

Section: Introductionmentioning

confidence: 98%

Phylogeny and evolutionary histories of Pyrus L. revealed by phylogenetic trees and networks based on data from multiple DNA sequences

Zheng

Cai

Potter

et al. 2014

Molecular Phylogenetics and Evolution

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“…(Jang et al 1991, 1992, Chevreau et al 1997). Nevertheless, their exact variation still remains unclear because isozyme markers are limited in number.…”

Section: Introductionmentioning

confidence: 99%

Identification of Asian Pear Varieties by SSR Analysis

et al. 2002

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Sixty Asian pear accessions from 6 Pyrus species were genetically identified by 9 SSR markers with a total of 133 putative alleles. Among them, 58 varieties could be successfully differentiated except for 2 pairs of synonymous or clonal varieties. All the SSR markers produced 1 or 2 discrete amplified fragments for all the diploid accessions, whereas a triploid variety showed 3 fragments with some SSRs. The number of putative alleles ranged from 7 to 20, with an average value of 14.8. The observed heterozygosity and the power of discrimination were 0.63 and 0.91, respectively. A phenogram based on the SSR genotypes was obtained, showing 3 major groups corresponding to the Japanese, Chinese and European groups. The SSR markers were highly polymorphic and could be utilized as a reliable tool for cultivar identification in Asian pears.

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Identification of Pyrus Species by Leaf Peroxidase Isozyme Phenotypes.

Cited by 18 publications

References 5 publications

Genetic diversity and similarity of pear (Pyrus L.) cultivars native to East Asia revealed by SSR (simple sequence repeat) markers

Genetic diversity and similarity of pear (Pyrus L.) cultivars native to East Asia revealed by SSR (simple sequence repeat) markers

Phylogeny and evolutionary histories of Pyrus L. revealed by phylogenetic trees and networks based on data from multiple DNA sequences

Identification of Asian Pear Varieties by SSR Analysis

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