Identification of QTL for dorso‐caudal chronic pleuritis in 12 crossbred porcine families

Gregersen, Vivi Raundahl; Sørensen, Kirsten Kørup; Christensen, Ole; Busch, Marie Erika; Vingborg, R. K. K.; Velander, Ingela; Lund, Mogens Sandø; Berthou, Christian

doi:10.1111/j.1365-2052.2010.02028.x

Cited by 13 publications

(17 citation statements)

References 26 publications

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“…Differences between lines of German Landrace and Hampshire pigs were used to map QTL for resistance to A. pleuropneumoniae in a F2 family, with the highest effects on SSC2 and SSC12 (Reiner et al 2014a), and to show associations between resistance and a broad range of differentially expressed genes (Reiner et al 2014b). The QTL identified in this study were in good agreement with those mapped in a study of slaughter pigs (Gregersen et al 2010).…”

Section: Introductionsupporting

confidence: 85%

“…Up to now, information on genetic resistance to pleuropneumonia has only been available as quantitative trait loci (QTL) mapped on the basis of microsatellite markers (Hoeltig et al 2009;Gregersen et al 2010;Sørensen et al 2011;Reiner et al 2014a, b). Gregersen et al (2010) found six QTL on SSC 2,8,12,13,14 and 18 in a study with 7470 animals, significant at a chromosome-wide level, when screening for dorso-caudal chronic pleuritis in slaughter pigs. Reiner et al (2014a) mapped 7 QTL for resistance to pleuropneumonia to SSC 2,6,12,13,16,17 and 18 in a F2 crossbred family (Hampshire × German Landrace).…”

Section: Discussionmentioning

confidence: 99%

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Candidate genes and gene markers for the resistance to porcine pleuropneumonia

et al. 2020

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Actinobacillus (A.) pleuropneumoniae is one of the most important respiratory pathogens in global pig production. Antimicrobial treatment and vaccination provide only limited protection, but genetic disease resistance is a very promising alternative for sustainable prophylaxis. Previous studies have discovered multiple QTL that may explain up to 30% of phenotypic variance. Based on these findings, the aim of the present study was to use genomic sequencing to identify genetic markers for resistance to pleuropneumonia in a segregating commercial German Landrace line. 163 pigs were infected with A. pleuropneumoniae Serotype 7 through a standardized aerosol infection method. Phenotypes were accurately defined on a clinical, pathological and microbiological basis. The 58 pigs with the most extreme phenotypes were genotyped by sequencing (next-generation sequencing). SNPs were used in a genome-wide association study. The study identified genome-wide associated SNPs on three chromosomes, two of which were chromosomes of QTL which had been mapped in a recent experiment. Each variant explained up to 20% of the total phenotypic variance. Combined, the three variants explained 52.8% of the variance. The SNPs are located in genes involved in the pathomechanism of pleuropneumonia. This study confirms the genetic background for the host's resistance to pleuropneumonia and indicates a potential role of three candidates on SSC2, SSC12 and SSC15. Favorable gene variants are segregating in commercial populations. Further work is needed to verify the results in a controlled study and to identify the functional QTN.

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Section: Introductionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Candidate genes and gene markers for the resistance to porcine pleuropneumonia

et al. 2020

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“…No QTL associated with ventro‐cranial chronic pleuritis have previously been reported. However, QTL for dorso‐caudal chronic pleuritis has been identified in the same family material but on the basis of a less dense SNP map (Gregersen et al. 2010).…”

Section: Resultsmentioning

confidence: 99%

“…A thorough description of the animals can be found in Christensen et al. (2010) and Gregersen et al. (2010).…”

Section: Methodsmentioning

confidence: 99%

Genomic regions associated with ventro-cranial chronic pleuritis in pig

Sørensen¹,

Gregersen²,

Christensen³

et al. 2011

Journal of Animal Breeding and Genetics

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Ventro-cranial chronic pleuritis can be a result of pleuropneumonia and enzootic pneumonia. These diseases cause severe losses in intensive pig production worldwide, but host resistance is difficult to breed for. It could be beneficial to use marker-assisted selection, and a step towards this is to identify genomic regions associated with the trait. For this purpose, 7304 pigs from 11 boar families were analysed for associations between single nucleotide polymorphisms and ventro-cranial chronic pleuritis. The pigs were genotyped by the use of the iSelect Custom 7 K porcine SNP Chip. Quantitative trait loci (QTL), significant at the chromosome-wide level, were identified on Sus scrofa chromosomes (SSC) 2, 4, 11, 12 and 13 in four different boar families. The QTL on SSC 4 in family G was also significant at the genome-wide threshold according to Bonferroni correction. We have identified a number of candidate genes, but the causative mutations still need to be identified. Markers closely associated with the resistance traits have a strong potential for use in breeding towards animals with improved characteristics concerning ventro-cranial chronic pleuritis.

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“…QTL on SSCs 2, 6, 12, 13, 16, 17 and 18, associated with resistance/susceptibility to App, were identified in controlled infection experiments in cross‐bred Hampshire/Landrace pigs and explained 6%–22% of variance in disease severity (Reiner, Bertsch, et al., ; Reiner, Dreher, et al., ). Another group found six QTL, also including SSCs 2, 12, 13 and 18, associated with dorso‐caudal chronic pleuritis (Gregersen et al., ). Combining chromosomal position with functional data could support the identification of functional candidate genes involved in App resistance or susceptibility.…”

Section: Prevention Control and Treatmentmentioning

confidence: 99%

Update onActinobacillus pleuropneumoniae-knowledge, gaps and challenges

Sassu

Bossé

Tobias

et al. 2017

Transbound Emerg Dis

146

178

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Summary Porcine pleuropneumonia, caused by the bacterial porcine respiratory tract pathogen Actinobacillus pleuropneumoniae, leads to high economic losses in affected swine herds in most countries of the world. Pigs affected by peracute and acute disease suffer from severe respiratory distress with high lethality. The agent was first described in 1957 and, since then, knowledge about the pathogen itself, and its interactions with the host, has increased continuously. This is, in part, due to the fact that experimental infections can be studied in the natural host. However, the fact that most commercial pigs are colonized by this pathogen has hampered the applicability of knowledge gained under experimental conditions. In addition, several factors are involved in development of disease, and these have often been studied individually. In a DISCONTOOLS initiative, members from science, industry and clinics exchanged their expertise and empirical observations and identified the major gaps in knowledge. This review sums up published results and expert opinions, within the fields of pathogenesis, epidemiology, transmission, immune response to infection, as well as the main means of prevention, detection and control. The gaps that still remain to be filled are highlighted, and present as well as future challenges in the control of this disease are addressed.

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Identification of QTL for dorso‐caudal chronic pleuritis in 12 crossbred porcine families

Cited by 13 publications

References 26 publications

Candidate genes and gene markers for the resistance to porcine pleuropneumonia

Candidate genes and gene markers for the resistance to porcine pleuropneumonia

Genomic regions associated with ventro-cranial chronic pleuritis in pig

Update onActinobacillus pleuropneumoniae-knowledge, gaps and challenges

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