Identification of QTLs and candidate genes for high grain Fe and Zn concentration in sorghum [Sorghum bicolor (L.)Moench]

Kotla, Anuradha; Phuke, Rahul M.; Krishna, Hare; Mehtre, S. P.; Rathore, Abhishek; Gorthy, Sunita; Srivastava, Rakesh K.; Das, Roma Rani; Prakash, Ashwin; Radhika, K.; Hash, C. Tom; Reddy, Belum V. S.; Patil, J. V.; Jabeen, Farzana; Shashikanth, D; Jaganathan, Jayakumar; Gaddameedi, Anil; Subhasini, Vangala; Deshpande, Santosh; Kumar, A Ashok

doi:10.1016/j.jcs.2019.102850

Cited by 27 publications

(14 citation statements)

References 22 publications

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“…Metal transporters, such as the P1B-ATPase family, zinc-regulated transporter (ZRT), iron-regulated transporter (IRT)-like protein (ZIP), natural resistance-associated macrophage protein (NRAMP) family, and cation diffusion facilitator (CDF) family, have been reported in plants, where these two metals play critical roles ( Colangelo and Guerinot, 2006 ). Several genes/gene families involved in Fe and Zn homeostasis such as yellow stripe-like (YSL), ZRT, ZIP, NRAMP, nicotianamine synthase (NAS), nicotianamine aminotransferase (NAAT), heavy metal ATPases (HMA), zinc-induced facilitator-like (ZIFL), metal tolerant protein (MTP), and others have been characterized in cereal crops such as rice ( Anuradha et al, 2012 ), sorghum ( Kotla et al, 2019 ). In pearl millet, PglYSL , PglZIP , and PglNRAMP and a single gene for PglFER and PglNAS were identified ( Mahendrakar et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Multi-Environment Quantitative Trait Loci Mapping for Grain Iron and Zinc Content Using Bi-parental Recombinant Inbred Line Mapping Population in Pearl Millet

Singhal

Satyavathi²,

Singh

et al. 2021

Front. Plant Sci.

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Pearl millet is a climate-resilient, nutritious crop with low input requirements that could provide economic returns in marginal agro-ecologies. In this study, we report quantitative trait loci (QTLs) for iron (Fe) and zinc (Zn) content from three distinct production environments. We generated a genetic linkage map using 210 F6 recombinant inbred line (RIL) population derived from the (PPMI 683 × PPMI 627) cross using genome-wide simple sequence repeats (SSRs). The molecular linkage map (seven linkage groups) of 151 loci was 3,273.1 cM length (Kosambi). The content of grain Fe in the RIL population ranged between 36 and 114 mg/Kg, and that of Zn from 20 to 106 mg/Kg across the 3 years (2014–2016) at over the three locations (Delhi, Dharwad, and Jodhpur). QTL analysis revealed a total of 22 QTLs for grain Fe and Zn, of which 14 were for Fe and eight were for Zn on three consecutive years at all locations. The observed phenotypic variance (R2) explained by different QTLs for grain Fe and Zn content ranged from 2.85 (QGFe.E3.2014–2016_Q3) to 19.66% (QGFe.E1.2014–2016_Q3) and from 2.93 (QGZn.E3.2014–2016_Q3) to 25. 95% (QGZn.E1.2014–2016_Q1), respectively. Two constitutive expressing QTLs for both Fe and Zn co-mapped in this population, one on LG 2 and second one on LG 3. Inside the QTLs candidate genes such as Ferritin gene, Al3+ Transporter, K+ Transporters, Zn2+ transporters and Mg2+ transporters were identified using bioinformatics approaches. The identified QTLs and candidate genes could be useful in pearl millet population improvement programs, seed, restorer parents, and marker-assisted selection programs.

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Section: Introductionmentioning

confidence: 99%

Multi-Environment Quantitative Trait Loci Mapping for Grain Iron and Zinc Content Using Bi-parental Recombinant Inbred Line Mapping Population in Pearl Millet

Singhal

Satyavathi²,

Singh

et al. 2021

Front. Plant Sci.

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“…Information on map positions of genes and linked markers on a chromosome are crucial for efficient determination of genetic architecture of polygenic traits in crop plants [42]. Several QTLs and candidate genes controlling resistance to Striga have been reported in cereals.…”

Section: Introductionmentioning

confidence: 99%

Identification of QTLs Controlling Resistance/Tolerance to Striga hermonthica in an Extra-Early Maturing Yellow Maize Population

et al. 2020

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Striga hermonthica parasitism is a major constraint to maize production in sub-Saharan Africa with yield losses reaching 100% under severe infestation. The application of marker-assisted selection is highly promising for accelerating breeding for Striga resistance/tolerance in maize but requires the identification of quantitative trait loci (QTLs) linked to Striga resistance/tolerance traits. In the present study, 194 F2:3 families of TZEEI 79 × TZdEEI 11 were screened at two Striga-endemic locations in Nigeria, to identify QTLs associated with S. hermonthica resistance/tolerance and underlying putative candidate genes. A genetic map was constructed using 1139 filtered DArTseq markers distributed across the 10 maize chromosomes, covering 2016 cM, with mean genetic distance of 1.70 cM. Twelve minor and major QTLs were identified for four Striga resistance/tolerance adaptive traits, explaining 19.4%, 34.9%, 14.2% and 3.2% of observed phenotypic variation for grain yield, ears per plant, Striga damage and emerged Striga plants, respectively. The QTLs were found to be linked to candidate genes which may be associated with plant defense mechanisms in S. hermonthica infested environments. The results of this study provide insights into the genetic architecture of S. hermonthica resistance/tolerance indicator traits which could be employed for marker-assisted selection to accelerate efficient transfer host plant resistance genes to susceptible genotypes.

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“…phenotypic variance of 9.42% and 5.82% for Zn and Fe, respectively. After validation, the linked markers identified in this study can be deployed for developing high-grain Fe and Zn cultivars in sorghum improvement programs globally (Kotla et al, 2019). To unravel the genetic determinants of natural variation observed for seed element concentration, Shakoor et al (2016) performed a GWA mapping of alleles regulating 20 traits influencing sorghum seed ionome, i.e., the mineral nutrient and trace element composition representing the inorganic component of cellular and organismal systems (Salt et al, 2008).…”

Section: Genomics: Qtl Mapping and Genome-wide Association Studiesmentioning

confidence: 99%

Genetic and genomic interventions in crop biofortification: Examples in millets

Kudapa

Barmukh²,

Vemuri³

et al. 2023

Front. Plant Sci.

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Micronutrient malnutrition is a serious threat to the developing world’s human population, which largely relies on a cereal-based diet that lacks diversity and micronutrients. Besides major cereals, millets represent the key sources of energy, protein, vitamins, and minerals for people residing in the dryland tropics and drought-prone areas of South Asia and sub-Saharan Africa. Millets serve as multi-purpose crops with several salient traits including tolerance to abiotic stresses, adaptation to diverse agro-ecologies, higher productivity in nutrient-poor soils, and rich nutritional characteristics. Considering the potential of millets in empowering smallholder farmers, adapting to changing climate, and transforming agrifood systems, the year 2023 has been declared by the United Nations as the International Year of Millets. In this review, we highlight recent genetic and genomic innovations that can be explored to enhance grain micronutrient density in millets. We summarize the advances made in high-throughput phenotyping to accurately measure grain micronutrient content in cereals. We shed light on genetic diversity in millet germplasm collections existing globally that can be exploited for developing nutrient-dense and high-yielding varieties to address food and nutritional security. Furthermore, we describe the progress made in the fields of genomics, proteomics, metabolomics, and phenomics with an emphasis on enhancing the grain nutritional content for designing competitive biofortified varieties for the future. Considering the close genetic-relatedness within cereals, upcoming research should focus on identifying the genetic and genomic basis of nutritional traits in millets and introgressing them into major cereals through integrated omics approaches. Recent breakthroughs in the genome editing toolbox would be crucial for mainstreaming biofortification in millets.

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Identification of QTLs and candidate genes for high grain Fe and Zn concentration in sorghum [Sorghum bicolor (L.)Moench]

Cited by 27 publications

References 22 publications

Multi-Environment Quantitative Trait Loci Mapping for Grain Iron and Zinc Content Using Bi-parental Recombinant Inbred Line Mapping Population in Pearl Millet

Multi-Environment Quantitative Trait Loci Mapping for Grain Iron and Zinc Content Using Bi-parental Recombinant Inbred Line Mapping Population in Pearl Millet

Identification of QTLs Controlling Resistance/Tolerance to Striga hermonthica in an Extra-Early Maturing Yellow Maize Population

Genetic and genomic interventions in crop biofortification: Examples in millets

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